Properties of phytochrome, Biology

Assignment Help:

Properties of Phytochrome

Phytochrome is a chromoprotein: this means it is composed of a protein and a chromophore. The chromophore is actually attached to the protein and is responsible for its-colour and light absorption. It is possible to isolate phytochrome in absolutely pure form by following the procedures of protein purification. The phytochrome in test-tubes also shows similar absorption characteristics. It absorbs red light (maximum absorption occurs at 660 nm wavelength) and on conversion, far-red light (maximum absorption occurs in far-red region at 730 nm). A typical absorption spectrum of phytochrome is shown-in figure. It is a soluble protein and is present in the cytoplasm. It is believed that Pfr form may be associated with the membranes. The intact phytochrome protein has a molecular weight of 124,000 daltons. .The total amino acid sequence of the protein is also known and, using recombinant DNA technology, the gene coding for phytochrome has been isolated.

1295_Properties of Phytochrome.png

Figure:  Absorption spectrum of phytochrome in vitro. Phytochrome was purified add its Absorption spectrum was measured by a spectrophotometer. When Pr was irradiated with red light, the absorption spectrum (dotted line) was obtained. This shows the conversion of Pr to Pfr.


Related Discussions:- Properties of phytochrome

Amphibians - regeneration in vertebrates, Amphibians - Regeneration in Vert...

Amphibians - Regeneration in Vertebrates Newts and salamander show remarkable regenerative ability in larval as well as adult stages. In larval stages except for limbs and tai

Define disadvantages of using yeast as a source of protein, Define Disadvan...

Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma

Explain marine oils, Marine oils These oils typically contain large amo...

Marine oils These oils typically contain large amounts of long - chain omega-3-polyunsaturated fatty acids, with up to six double bonds and they are usually rich in vitamins A

How to check quality of grains, Q. How to check Quality of grains? Food...

Q. How to check Quality of grains? Food grains for human consumption are whole or broken kernels of cereals, millets and pulses. According to PFA, they shall be free from added

The seven levels of taxonomy, how are the listed from largest group to smal...

how are the listed from largest group to smallest group

Explain the factors affecting the process of deep fat frying, Factors affec...

Factors affecting the process of deep fat frying. The common factors influencing the process of deep frying include: 1.  Heat- Frying temperatures ranging from 150 -190°C

Metamorphosis in amphibians, Metamorphosis in Amphibians Metamorphosi...

Metamorphosis in Amphibians Metamorphosis is radical in anurans, slight or not exists in urodeles. In anuran amphibians like toads and most frogs, metamorphosis is generally

What is vena cava, What is vena cava? Which type of blood circulates within...

What is vena cava? Which type of blood circulates within the vena cava? The vena cava are either of two large veins that debouch into the right atrium. The superior vena cava d

What is available soil nutrient, Availability of nutrients in soils  Th...

Availability of nutrients in soils  The productivity of a crop depends largely on the nutrients discussed in the previous section. It is important to find out methods which wil

Risk analysis - in-vitro studies, In-vitro studies Mechanistic  data mi...

In-vitro studies Mechanistic  data might be  supplemented by  data  from in-vitro  studies, like  as information on genotoxicity derived from reversion assays or other same ass

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd