Properties of phytochrome, Biology

Assignment Help:

Properties of Phytochrome

Phytochrome is a chromoprotein: this means it is composed of a protein and a chromophore. The chromophore is actually attached to the protein and is responsible for its-colour and light absorption. It is possible to isolate phytochrome in absolutely pure form by following the procedures of protein purification. The phytochrome in test-tubes also shows similar absorption characteristics. It absorbs red light (maximum absorption occurs at 660 nm wavelength) and on conversion, far-red light (maximum absorption occurs in far-red region at 730 nm). A typical absorption spectrum of phytochrome is shown-in figure. It is a soluble protein and is present in the cytoplasm. It is believed that Pfr form may be associated with the membranes. The intact phytochrome protein has a molecular weight of 124,000 daltons. .The total amino acid sequence of the protein is also known and, using recombinant DNA technology, the gene coding for phytochrome has been isolated.

1295_Properties of Phytochrome.png

Figure:  Absorption spectrum of phytochrome in vitro. Phytochrome was purified add its Absorption spectrum was measured by a spectrophotometer. When Pr was irradiated with red light, the absorption spectrum (dotted line) was obtained. This shows the conversion of Pr to Pfr.


Related Discussions:- Properties of phytochrome

Use pcr to sequence the particular gene, You wish to use PCR to sequence a ...

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu

Atp is directly requiredin which processes in muscle, ATP is DIRECTLY requi...

ATP is DIRECTLY required in which of the following processes in muscle? A. Net flux of sodium ions from intracellular space to extracellular space. B. Detachment of myosin h

Compute the osmolarity of a solution, Calculate the osmolarity of a solutio...

Calculate the osmolarity of a solution of 5% dextrose in water (dextrose = glucose, molec. Wt. = 180).

Etiological factors contributing to lactose intolerance, Q. Etiological fac...

Q. Etiological factors contributing to lactose intolerance? The etiological factors contributing to lactose intolerance include: • Genetic factor • Reduction in jejunal

What is the connection between tissue fluid and plasma, What is the connect...

What is the connection between tissue fluid, plasma and lymph? Tissue fluid is plasma (minus its proteins) which has leaked out of the capillaries. Lymph is tissue fluid which

Why binding motifs regulate gene expression, How do leucine zipper binding ...

How do leucine zipper binding motifs regulate gene expression?

Carbomedics valve-types of valves, Carbomedics Valve :  Almost simila...

Carbomedics Valve :  Almost similar to StJude Medical valve, his is a low profile bileaflet valve made of pyrolitic carbon. On echo cardiography four small jets of regurg

How are solutions classified, Q. How are solutions classified as per to the...

Q. How are solutions classified as per to their comparative tonicity? Comparative to another, a solution can be isotonic or isosmotic, hypotonic or hyposmotic or hypertonic or

What is the heterophagic intracellular digestion, Q. What is the heterophag...

Q. What is the heterophagic intracellular digestion and How is this process accomplished? Heterophagic intracellular digestion is the breaking into smaller substances of extern

What are typical fauna of the tropical forests, What are the typical vegeta...

What are the typical vegetation and the typical fauna of the tropical forests? In the vegetation of a tropical forest broadleaf evergreen trees predominate. On the top of the t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd