Properties of phytochrome, Biology

Assignment Help:

Properties of Phytochrome

Phytochrome is a chromoprotein: this means it is composed of a protein and a chromophore. The chromophore is actually attached to the protein and is responsible for its-colour and light absorption. It is possible to isolate phytochrome in absolutely pure form by following the procedures of protein purification. The phytochrome in test-tubes also shows similar absorption characteristics. It absorbs red light (maximum absorption occurs at 660 nm wavelength) and on conversion, far-red light (maximum absorption occurs in far-red region at 730 nm). A typical absorption spectrum of phytochrome is shown-in figure. It is a soluble protein and is present in the cytoplasm. It is believed that Pfr form may be associated with the membranes. The intact phytochrome protein has a molecular weight of 124,000 daltons. .The total amino acid sequence of the protein is also known and, using recombinant DNA technology, the gene coding for phytochrome has been isolated.

1295_Properties of Phytochrome.png

Figure:  Absorption spectrum of phytochrome in vitro. Phytochrome was purified add its Absorption spectrum was measured by a spectrophotometer. When Pr was irradiated with red light, the absorption spectrum (dotted line) was obtained. This shows the conversion of Pr to Pfr.


Related Discussions:- Properties of phytochrome

Explain the evaluation of the perimplant marginal tissues, Explain the Eval...

Explain the Evaluation of the Perimplant Marginal Tissues This is to be followed by Evaluation of the Perimplant Marginal Tissues: This includes the following parameters: 1)

Polar bear is adapted to its environment, Give three ways in which a polar ...

Give three ways in which a polar bear is adapted to its environment. A polar bear is adapted to its environment by its high ratio of bulk to surface area, a thick water-repelle

Ammonia assimilation, Ammonia Assimilation - Inorganic Nitrogen and Sulphur...

Ammonia Assimilation - Inorganic Nitrogen and Sulphur Metabolism Nitrogen (N 2 ) gas and NO 3 are the most common available forms of inorganic nitrogen. Both are enzymaticall

Nervous tissue, This constitutes about 2.4% of the body weight of man viz. ...

This constitutes about 2.4% of the body weight of man viz. Brain-1400 gram Spinal nerve-150 gram Spinal cord-30 gram Cranial nerve-10 gram C.S.F. is altogether not present in

Unified cell theory, Unified Cell Theory The present unified cell theo...

Unified Cell Theory The present unified cell theory states that All living things are composed of either a single Cell or of the cells and cell products. The cell is a m

Define mechanism for copper absorption, Define Mechanism for Copper Absorpt...

Define Mechanism for Copper Absorption? As in the case of other minerals, copper absorption appears to occur by two mechanisms: i) Saturable active mechanism, which operates

Build a genomic dna library, Construct a genomic DNA library with the help...

Construct a genomic DNA library with the help of some methylaee and finall do viral packaging.

West nile virus infection, West Nile virus infection West Nile virus (W...

West Nile virus infection West Nile virus (WNV) is a type strain of flaviviruses and is related to Japanese encephalitis group. The virus was first isolated from a woman in the

Orientation to the paediatric unit - child care, Orientation to the Paediat...

Orientation to the Paediatric Unit   When the family arrives at the pediatric unit, they should be shown the room where their child will stay and the toilet facilities, the fan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd