Properties of phytochrome, Biology

Assignment Help:

Properties of Phytochrome

Phytochrome is a chromoprotein: this means it is composed of a protein and a chromophore. The chromophore is actually attached to the protein and is responsible for its-colour and light absorption. It is possible to isolate phytochrome in absolutely pure form by following the procedures of protein purification. The phytochrome in test-tubes also shows similar absorption characteristics. It absorbs red light (maximum absorption occurs at 660 nm wavelength) and on conversion, far-red light (maximum absorption occurs in far-red region at 730 nm). A typical absorption spectrum of phytochrome is shown-in figure. It is a soluble protein and is present in the cytoplasm. It is believed that Pfr form may be associated with the membranes. The intact phytochrome protein has a molecular weight of 124,000 daltons. .The total amino acid sequence of the protein is also known and, using recombinant DNA technology, the gene coding for phytochrome has been isolated.

1295_Properties of Phytochrome.png

Figure:  Absorption spectrum of phytochrome in vitro. Phytochrome was purified add its Absorption spectrum was measured by a spectrophotometer. When Pr was irradiated with red light, the absorption spectrum (dotted line) was obtained. This shows the conversion of Pr to Pfr.


Related Discussions:- Properties of phytochrome

Chromosomes and molecular homology, CHROMOSOME S AND MOLECULAR HOMOLOGY - ...

CHROMOSOME S AND MOLECULAR HOMOLOGY - The DNA content of diploid cells is similar. Hoyer et al in 1965 showed that DNA of humans was 100% similar with chimpanzee and 94%

Tissue culture, Tissue culture The ability of a plant cell to give r...

Tissue culture The ability of a plant cell to give rise to a whole plant is called totipotency. The culture of a cell or a tissue in a suitable medium to produce new plan

Explain evolutionary relationship among animal groups, Evolutionary relatio...

Evolutionary relationship among various animal groups is depicted in the cladogram given below. P, Q and R respectively shown: a.  Vertebrae, hair and four limbs. b.  B

Why low ca++ in the body fluid cause in body, Low Ca ++ in the body fluid ...

Low Ca ++ in the body fluid may be the cause of: 1.  Tetany 2. Anaemia 3. Angina pectoris 4. Gout  Tetany cause low ca ++

Hydrogen bonds important for making water cohesive liquid, Hydrogen bonds a...

Hydrogen bonds are important for all of the following except:: a) Allowing carbohydrates to dissolve in water b) Stabilizing the three-dimensional shape of proteins. c) Ma

Elaborate immune response system and their categories, Elaborate Immune Res...

Elaborate Immune Response system and their categories? The immune response is a group of defenses that the body has developed to combat disease-producing agents, or pathogens.

Radiation sterilization, introduction of an assignment about radiation ster...

introduction of an assignment about radiation sterilization

Explain the balance of the ecosystem, Some people believe that being a vega...

Some people believe that being a vegan is the most responsible way to get nutrients while still maintaining the balance of the ecosystem. Explain this rationale, giving solid infor

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd