Process of determination of vitamin B12, Biology

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Determination of vitamin B12

Spectrophotometric determination of the characteristic ultraviolet absorption is suitable for the determination of pure vitamin B12 or of pure aqueous solution in the absence of interfering impurities. Microbiological assay is performed by any of the following:

  • Determination of the diffusion in the cup-plate test using a mutant of Escherichia coli ATCC 10799 as the test organism
  • Titrimetric determination of the acid produced in the tube test using Lactobacillus lecihmannii ATCC 7830.

 


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