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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
Under which forms is the Trypanosoma cruzi found in its hosts? In the definitive hosts as well as in triatomine bugs (middle hosts) the protozoan that causes Chagas' disease ch
classification of virus
Crustacea - Feeding and Digestion in Arthropods Branchiopods (Anacostraca) give a good instance for filter feeding mechanism. The appendages are of foliaceous type (leaf-like)
Define Criteria for Assessment of Vitamin B 12 Status? Low serum or plasma levels of vitamin B 12 should be the first indication of poor status and this could be confirmed
List the five major phases of the cell cycle, and briefly explain what happens during each phase. G1 phase: the cell grows. S phase: DNA is copied. G2 phase: the cell prepares
Define Comprehensive Dietary Management Regime of Patient? A comprehensive dietary management regime of the patient should be based on the patient profile as gathered pre and p
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How different are reptiles and birds concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic, i.e., they do not control thei
phylogenetic consideration
How can we control the disease Cholera Control: Individuals infected with cholera require oral rehydration therapy with NaCl plus sucrose, sodium bicarbonate and potassium ch
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