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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
poor metabolism phenotype will have
Viscosity The viscosity at temperatures above its gelation point is relatively constant at pH values of 4.5 to 9.0 and is not greatly affected by age or ionic strength within
What is the meaning of Body composition? It refers primarily to the distribution of muscle, fat, bone and other tissues in the body, and its measurement is often considered as
MYCOPLASMOSIS Mycoplasma organisms are the smallest free living, lack of cell-wall and bounded by a triple layer of plasma membrane. The organisms are pleuomorphic in shape, viz.
Define salient modifiable factors which affect birth weight? The following are the salient modifiable factors which affect birth weight and gestation adversely: Iron deficiency
Briefly describe fluid feeders
Q. Causes of Non-Ketotic Hyperosmolar Diabetic Coma? The causes of NKHDC are given below: 1) Infections 2) Trauma 3) Burns 4) Myocardial Infarctions (heart attack)
in what part of the human body aschelminthes found?
You observe the following numbers of individuals of each genotype in a population: AA 700 Aa 200 aa 100 a.) what are the observed genotype frequencies in this populaiton?
What is cellular regeneration? How is mitosis related to this process? Some tissues are able to regenerate when injured. The liver, for instance, regenerates when small pieces
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