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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
Use of biotechnology in characterization of animal biodiversity. Genetic uniqueness of populations is measured by the relative genetic distances of such populations from each o
Aeolian Wind transported materials constitute this category. This can be further divided into dunes or loess. Dunes are found in thr& types of situations. Fitly, these may occu
Let us now understand the definition, causes, signs and symptoms, diagnosis and treatment of hypoglycaemia. It is very important for you to learn about it because patient will be h
what are the excretory organs of birds
Secondary Production - Ecosystem The net primary production is the only energy available to consumers or heterotrophs including man. Herbivores such as cow or deer gaze upon
Feeding mechanism in holozoic organisms
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Explain the Predator-Prey Relationships ? The feeding by a population of one species upon members of another species is referred to as predation. Many scientists also consider
What are histogenesis and organogenesis? Histogenesis is the procedure of tissue formation in the embryonic development. Organogenesis is the process of organ formation. Before
Explain Itraconazole It has a broader spectrum of activity than fluconazole. It is active against a wide variety of fungi including Cryptococcus neoformans, Aspergillus spp., B
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