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You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mutations, that lead to differential expression of the mutated gene in different tissues. For a variety of reasons, you cannot, or do not wish to use micro-array technology. So, you choose to start by measuring by some other means the mRNA levels in a variety of tissues.
What is a good combination of techniques that will allow you to rapidly quantify the amounts of multiple RNA species and to give some indication of the amounts of specific messages (transcripts of genes)? You do have the ability to do many types of PCRs and also have an assortment of DNA probes that are known to effectively hybridize to regions of mRNAs that may contain the mutated regions of interest.
What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted mostly of molecular nitrogen (N2) and molecular oxygen
What are the four characteristics that all chordates have at some stage of their lifecycle ?
These are found in about 3-6 per cent of cases of ARF. These nodules are typically subcutaneous, firm, painless, freely movable (0.5-2 cm size) and their presence indicates that t
Q Annelid Identity card. How are they characterized according to examples of representing beings, type of symmetry, basic morphology, germ layers and coelom, respiratory system, di
Permanent Partial Disability - Injury from an Accident Permanent partial disability restrains the worker by doing the job he has been doing. He recovers from the injury in th
Explain the term- Horizons These processes take a long time- may be a few thousand years to create a soil. As the time passes, the soil matures and generally becomes deeper and
porifera
Trypsin Trypsin is secreted by the pancreas in an inactive form trypsinogen. It is activated by enterokinase secreted by the glands in the intestinal wall. As trypsin is forme
Insect killing jar Secure a wide-mouth glass jar with a screw top or single which closes very tightly. Place a wad of cotton in the bottom and cover it with a round piece of ca
What is predatism? Predatism is the ecological interaction in which one individual mutilates or kills another to get food. Predatism is an inharmonious (negative) ecological i
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