Membrane protein purification and reconstitution, Biology

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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


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