Membrane protein purification and reconstitution, Biology

Assignment Help:

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


Related Discussions:- Membrane protein purification and reconstitution

What are mutagenic agents, What are mutagenic agents? The Mutagenic age...

What are mutagenic agents? The Mutagenic agents, or mutagens, are physical, chemical or biological factors that can cause alteration in DNA molecules. Instances of believed

Kangroo rat, Kangroo Rat Kangroo rat Dipodornys merriami, a native of ...

Kangroo Rat Kangroo rat Dipodornys merriami, a native of South-West America is a classical example of how small mammals survive in desert. It exhibits all the osmoregulatoryad

Explain surface properties of proteins, Explain Surface properties of prote...

Explain Surface properties of proteins The surface properties relates primarily to surface tension, a) Emulsification  and b) Foaming characteristics of proteins,

Explain about the invert sugar, Explain about the Invert sugar? Invert ...

Explain about the Invert sugar? Invert sugar is sucrose, which can be hydrolysed to split the disaccharide into its component sugars, fructose and glucose. It is known as inver

Define erythropoietin, Erythropoietin (EPO) A. acts by increasing the p...

Erythropoietin (EPO) A. acts by increasing the production of red blood cells by cells in the bone marrow. B. is secreted by peritubular interstitial cells of the kidney cort

What are the functional properties of gums, What are the functional propert...

What are the functional properties of gums that are considered to be important in food industries? The functional properties of gums that  are considered to be important in foo

Five kingdom classification, discuss the merits nd demerits of five kingdom...

discuss the merits nd demerits of five kingdom classification.

Describe the genetic material of a virus, Q What is the genetic material of...

Q What is the genetic material of a virus? How does that material act in viral reproduction? There is DNA viruses double strand or single strand DNA and RNA viruses double stra

Describe the monomer unit of a protein, Describe the monomer unit of a prot...

Describe the monomer unit of a protein and how monomer units are assembled into peptides.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd