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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
What are cilia and flagella? Cilia and flagella are structures found in prokaryotes as well in eukaryotic cells. They play defense, nutrition and movement roles for the cell. I
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The name Joseph Merrick, subject of "The Elephant Man", is generally associated with one disorder, though it now seems likely he actually suffered from a different disorder. Name e
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