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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Oseltamivir (Tamiflu) This oral neuraminidase inhibitor, started within 36 hours of symptom onset, can decrease the severity and duration of symptoms caused by either influ
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The reported occurrence of ICMAs is 1.2 per cent to 5 per cent of cases. Streptococci and S.aureus account for 50 per cent and 10 per cent of cases, respectively, and are seen with
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Microbodies Peroxisomes and glyoxysomes are microbodies formed from ER which superficially resemble the lysosomes. Peroxisomes are spherical bodies limited by a single membrane
What does ecological niche of an organism represent? Mention the scientific term used for modified form of reproduction in which seeds are produced without fusion of gametes.
What are enzyme cofactors? Some enzymes require other associated molecules to work. These molecules are known as enzyme cofactors and they can be, for example, organic ions lik
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