Membrane protein purification and reconstitution, Biology

Assignment Help:

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


Related Discussions:- Membrane protein purification and reconstitution

Explain factors influencing lipid oxidation, Factors Influencing Lipid Oxid...

Factors Influencing Lipid Oxidation  Food lipids contain a variety of fatty acids that differ in chemical and physical properties and also in their susceptibility to oxidation.

Define arterial vessels - arterioles and arteries, Q. What are arterial ves...

Q. What are arterial vessels, arterioles and arteries? Arterial vessels are every blood vessel that carries blood from the heart to the tissues, arterioles and Arteries are art

Explain cori cycle , Under the limiting oxygen conditions experienced in ...

Under the limiting oxygen conditions experienced in by vigorous exercise, the configuration of NADH by glycolysis exceeds the ability of the respiratory chain to oxidize it back to

Why ascending method of paper chromatography is preferred, Why Ascending Me...

Why Ascending Method of Paper Chromatography is Preferred? The ascending method is preferred because of the simplicity of the set up. In actual method the substance to be separ

Determine the toe corticospinal interneuron, Which of the following is true...

Which of the following is true for a toe corticospinal interneuron that produces action potentials during movements of the big toe of the left foot? A. A portion of its axon is

Define changes in gluten proteins during dough formation, Define Changes in...

Define Changes in Gluten Proteins during Dough Formation? Initially, gluten is formed when flour and water are mixed together. The proteins in the flour, glutenin and gliadin c

What are social dangers, Members belonging to the scientific community fear...

Members belonging to the scientific community fear the misuse of this therapy leading to dangerous consequences.  People may try to insert the desired gene, for example, the gene f

The hydrogen ion concentration of stomach, What is the hydrogen ion concent...

What is the hydrogen ion concentration of stomach acid pH=1.6?

Nutrition of embryo sac, Nutrition of Embryo Sac The morphology of th...

Nutrition of Embryo Sac The morphology of the ovule suggests that the chalazal end is the main route for the entry of nutrients. The funicular vascular supply ends at the bas

What are analogies for a nucleolus, What are analogies for a nucleolus? ...

What are analogies for a nucleolus? If the nucleolus is the president of a factory then the nucleolus is the manager

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd