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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
57. While planning for an artificial hybridisation programme involving dioecious plants, which of the following steps will be irrelevant A usting of pollen on stigma B Collection
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Honey comes under the purview of Prevention of Food Adulteration Act (PFA). Due to its limited production and high cost, honey is prone to adulteration by cane sugar, invert sy
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