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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Explain about the Cyanocobalamin (vitamin B 12 )? Vitamin B 12 (cobalamin, cbl) is a unique vitamin in human nutrition, since its malabsorption leads to the fatal syndrome of
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If a lipid bilayer does not form in a test tube of phospholipids in a liquid solvent and instead the heads join together in a circular pattern, the most likely explanation is: a
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