Membrane protein purification and reconstitution, Biology

Assignment Help:

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


Related Discussions:- Membrane protein purification and reconstitution

What are the parasympathetic neurons, What are the parasympathetic neurons ...

What are the parasympathetic neurons Patient A has a disease that has destroyed half of the alpha-adrenergic receptors on each of the smooth muscles that control the diameter o

Pressure gradient, Pressure Gradient You know that air pressure is the ...

Pressure Gradient You know that air pressure is the weight of the atmosphere over a unit area of the earth's surface. The average air pressure at sea level is approximately 1 k

Aim of diabetes counselling, Aim of Diabetes Counselling Diabetes is a...

Aim of Diabetes Counselling Diabetes is a life-long illness. Diagnosis of diabetes has psychological and physical implications. Preventive counselling and life style change ca

Explain in brief about cytokines, Cytokines activate inflammatory cells lik...

Cytokines activate inflammatory cells like neutrophils and eosnophils.IL-5 is important in activation of eosnophils, IL-4 enhances IgE production by B-cells. IL-4, Il-5, IL-6, TNF-

Natural control of population growth, N A TURA L CONTROL OF POPULATION G...

N A TURA L CONTROL OF POPULATION GROWTH - 1 .       Biotic potential - It is capacity of population to increase its number by reproduction. For all animals, it is v

Describe integrative systems of the body, Q. Why is the endocrine system co...

Q. Why is the endocrine system considered one of the integrative systems of the body? What is the other physiological system that also has this function? The endocrine system i

Dehydration reaction between both molecules, Draw decanol, plamitic acid (C...

Draw decanol, plamitic acid (C16:0 fatty acid) and the resulting wax ester generated by a dehydration reaction between both molecules

State the term autopsy data - brain patient, State the term Autopsy data - ...

State the term Autopsy data - Brain patient Autopsy data are not always entirely usable for validation purposes, in that numerous changes may have taken place in the patient's

Describe the physiology of atrial septal defect, Describe the Physiology of...

Describe the Physiology of atrial septal defect? In ASD the magnitude of left to right shunt is determined by the size of the defect and the relative compliance of the right ve

What are passive and active immunization, What are passive and active immun...

What are passive and active immunization? According to the duration of the protection how do these types of immunization differ? Active immunization is that in which an antigen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd