Membrane protein purification and reconstitution, Biology

Assignment Help:

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


Related Discussions:- Membrane protein purification and reconstitution

Zoogly, are protozoans primitive to life .

are protozoans primitive to life .

Explain glucocorticoids, Explain Glucocorticoids The adrenal cortex se...

Explain Glucocorticoids The adrenal cortex secretes the glucocorticoids which  increase gluconeogenesis by increasing hepatic intake of amino acids accompanied by increased ac

What do you mean by tuberculosis, Q. What is tuberculosis? How is the disea...

Q. What is tuberculosis? How is the disease transmitted? Is there treatment for tuberculosis? The Tuberculosis is a disease caused by the Mycobacterium tuberculosis bacteria wh

Determine what is dermal branchiae, Determine what is dermal branchiae? ...

Determine what is dermal branchiae? External extensions of outer epidermis and peritoneum of the echinoderm body cavity. Both outer epidermis and inner peritoneum are lined wit

Deficiency diseases-selenium and vitamin e deficiency, Selenium and vitamin...

Selenium and vitamin E deficiency Selenium is an essential micro-mineral required for various physiological functions and for immunity against a variety of diseases. It is an i

Obelia, why is it consideredto have an intermideate grade oforganisation

why is it consideredto have an intermideate grade oforganisation

Define absorption, Define Absorption, Storage and Elimination of ascorbic a...

Define Absorption, Storage and Elimination of ascorbic acid? Ascorbic acid is rapidly absorbed from the intestines primarily by active transport.  Simple diffusion or carrier-m

Explain about the food product development, Explain about the Food product ...

Explain about the Food product development? Food product development is often commodity related. This type of research needs to be carried out in the pilot plants with the equi

Functions of cell wall, FUNCTIONS OF CELL WALL 1.       Provides shape ...

FUNCTIONS OF CELL WALL 1.       Provides shape to plant cell rigidity to cells. 2.       Functions as a barrier to entry of pathogens into the cells. 3.       Provides pr

Explain control of oedema in nutritional care, Explain Control of Oedema in...

Explain Control of Oedema in Nutritional Care? Low levels of circulating proteins lead to oedema due to loss of colloidal osmotic pressure to maintain the normal fluid shift me

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd