Membrane protein purification and reconstitution, Biology

Assignment Help:

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly achieved by adding a detergent that solubilizes the membrane.  In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS.  they readily  intercalate  into  the  lipid  disrupt & bilayer  the  hydrophobic  interactions as  the  detergent  molecules  are  themselves  amphipathic  .

 

1140_22.png

figure:  Structures of (a) Triton X-100 and (b) octyl glucoside.

 

924_22.png

figure:  (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.


Related Discussions:- Membrane protein purification and reconstitution

Explain the toxicity of nicotinic acid, Explain the Toxicity of nicotinic a...

Explain the Toxicity of nicotinic acid? Although therapeutically useful in lowering serum cholesterol, administration of chronic high oral doses of nicotinic acid can lead to h

Determine the exercises require to do diabetic patient, Determine the Exerc...

Determine the Exercises require to do diabetic patient Exercises are any bodily activity that enhances or maintain physical fitness and overall health. It is performed for stre

Egestion of food, EGESTION To give out undigested food from alimenta...

EGESTION To give out undigested food from alimentary canal. Faeces can remain in colon for 36 hrs. then moving into rectum by gastro-colic reflex. Faeces consists of 3

Male reproductive disorders-cryptorchidism, Cryptorchidism Cryptorchid...

Cryptorchidism Cryptorchidism or incomplete descent of testis or retention of testis in the abdominal cavity occurs in all domestic species but is seen most commonly in stalli

Why is blood significant for larger animals, What is the alternative means ...

What is the alternative means for transport of substances in animals without a circulatory system? Why is blood significant for larger animals? In animals that do not present t

Tanco beans plant, what are the origin, morphology, active constituents, us...

what are the origin, morphology, active constituents, uses and market preparation of tanco beans

Physical layout - neonatal care unit, PHYSICAL LAYOUT In this section ...

PHYSICAL LAYOUT In this section we shall focus on location floor plan, ventilation, lighting environmental temperature and humidity, acoustic characteristic, communication sy

Dfine integrated child development services (icds), Dfine Integrated Child ...

Dfine Integrated Child Development Services (ICDS)? The adolescent girl scheme under ICDS intends to cover school dropout girls, 11-18 years in age with a view to meet their ne

Do you know about lateral lines of fishes, Q. What are the lateral lines of...

Q. What are the lateral lines of fishes? The lateral lines of bony fishes are sense organs that extend along both sides of the animal body they make contact with the environmen

Transport of substances done across the bryophyte tissue, How is the transp...

How is the transport of substances done across the bryophyte tissues? How is this feature related to the general size of these plants? In bryophytes there are no water-conduct

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd