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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
What are biogeochemical cycles? Biogeochemical cycles are representations of the circulation and recycling of matter in nature. The major biogeochemical cycles studied in Ec
Define about the Geriatric Nutrition? Aging has been defined as "a series of time related process that ultimately brings life to a close." Persons of 60 years of age and older
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Soaking Soaking is an another inexpensive processing method, which does not require much technical know how, is commonly practiced for improving the palatability of chaffed cro
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Explain Technical factors influencing food production? Improvement in technology has a significant impact on productivity. Improvements may occur in seed production, fertilizer
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