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Which of the following statements regarding the separation of DNA fragments using gel electrophoresis is false?
A. DNA fragments will migrate towards the positively charged electrode (anode) and are divided by relative size.
B. Smaller DNA fragments migrate faster through the agarose gel while larger fragments migrate more slowly therefore fragments at the bottom of the gel are smaller than those at the top of the gel.
C. A pH gradient is formed within the agarose gel allowing for the separation of DNA fragments based on their isoelectric point.
D. Ethidium bromide (added to the DNA sample, gel and buffer) can intercalate within the DNA double helix and can fluoresce when exposed to UV light therefore allowing for the DNA fragments to be visualized.
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