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Explain Simple Staining Technique?
Here single staining agent is used to determine the size, shape and arrangement of bacterial cells. It is simple and easy to perform. Dried suspension of microorganisms on slide is flooded with a dilute solution of a dye e.g. carbol fuchsin or crystal violet for 1-2 minutes. It is then rinsed several times in water to remove excess stain and is blotted dry.
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Describe three mechanisms cells use to produce diversity of ion channel structure and function.
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