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There are three steps of PCR
a) Denaturation. The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands which can act as templates for DNA synthesis.
b) Primer annealing. The combination is rapidly cooled to a described temperature that allows the two primers to bind to the sequences on each of the two strands flanking the goal DNA. This annealing temperature is measured carefully to ensure in which the primers bind only to the desired DNA sequences. Every primer binds to each strand. Two parental strands do not reanneal with each other because the primers are in huge excess over parental DNA.
c) Elongation. The temperature of the mixture is raised to 72°C (commonly) and kept at this temperature for a preset period of time to permits DNA polymerase to elongate each primer by copying the single-stranded templates. At the last of this incubation both single-stranded template strands have been made partially double stranded. The new strand of each double-stranded DNA extends for a variable distance downstream
The various organs involved in the digestion process are: Alimentary tract which includes mouth, pharynx, esophagus stomach, small intestine, large intestine, appendix, rec
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Define the Poverty and hunger - mutually causes, devastating effects? Measures of food deprivation, nutrition and poverty are strongly correlated. Countries with a high prevale
Stability studies are req. for the product stable at their desire time as per specification , which give the expiry date and quality as per the manner.
WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL
Explain the Techniques of Numerical Taxonomy Many other techniques of numerical taxonomy are available now, some with special objectives. Classification based on the methods
Explain the Spectrophotometer or Colorimete? These can be used to measure the microbial growth or can be used in various microbial and biochemical assays. Figure illustrates t
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