How do you verify that a certain gene encodes a certain prot, Biology

Assignment Help:
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains the gene encoding the protein. The last part of the question is where I am stuck which says: how would you verify that one of the genes you isolated does indeed encode a protein with the catalytic properties of the protein being studied?

Can someone please give me a good, detailed explanation as to an experiment I could do to verify that the gene I cloned encodes the protein I am interested in?

Related Discussions:- How do you verify that a certain gene encodes a certain prot

Define about the paper electrophoresis, Define about the Paper electrophore...

Define about the Paper electrophoresis? In paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate moistened with buffer solution

Chronic complications of diabetes, Q. Chronic complications of diabetes? ...

Q. Chronic complications of diabetes? These occur gradually when the diabetics do not monitor the blood sugar and they are careless about eating, exercise and the medications a

History - assessment of patents with cardiovascular probems, History P...

History Past and present history of cardiovascular problems of patient & family. History of chest pain, shortness of breath, fatigue, abnormal skin colour, dizziness, vertigo

What is available soil nutrient, Availability of nutrients in soils  Th...

Availability of nutrients in soils  The productivity of a crop depends largely on the nutrients discussed in the previous section. It is important to find out methods which wil

How many types of gametes can be formed, How many different types of gamete...

How many different types of gametes can be formed by individuals of the following genotypes? What are they in each case a) AaBb b) AaBB c) AaBbCc d) AaBBCc e) AaBbcc f) AaBbCcDdEe

Antibody structure, Antibody Structure: An antibody molecule consist of...

Antibody Structure: An antibody molecule consist of two alike light chains ( 220  amino acids each) ad two similar heavy chains (about  440-450  amino acids each) held together

Culture negative endocarditis, In patients who have not received prior anti...

In patients who have not received prior antibiotics and who will ultimately have blood culture positive endocarditis, it is likely that 95 - 100 per cent of all cultures obtained w

Carrier protein in a plasma membrane, What is a characteristic feature of a...

What is a characteristic feature of a carrier protein in a plasma membrane? Carrier proteins are globular proteins which are particular it their action and therefore regulate t

What are antifoaming agents, What are Antifoaming Agents In food indust...

What are Antifoaming Agents In food industries, undesirable foams may be  formed such as in concentration of fruit juices, coffee extracts, vegetable oils and syrups or in ferm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd