How do you verify that a certain gene encodes a certain prot, Biology

Assignment Help:
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains the gene encoding the protein. The last part of the question is where I am stuck which says: how would you verify that one of the genes you isolated does indeed encode a protein with the catalytic properties of the protein being studied?

Can someone please give me a good, detailed explanation as to an experiment I could do to verify that the gene I cloned encodes the protein I am interested in?

Related Discussions:- How do you verify that a certain gene encodes a certain prot

Bacteria entering the blood system, Briefly describe the principal lines of...

Briefly describe the principal lines of defence against bacteria entering the blood system. A blood clot forms a barrier to entry by bacteria. White cells ingest and kill bacte

Captive breeding - measures for species conservation, Captive Breeding - Me...

Captive Breeding - Measures for Species Conservation Species which are reduced to dangerous levels need more intensive management, and one strategy is their captive breeding.

Mode of nutrition , What is the mode of nutrition in fish,human,amoeba,scor...

What is the mode of nutrition in fish,human,amoeba,scorpian & toad

Transcription of a eukaryotic protein-coding gene, 1. Describe how the vari...

1. Describe how the various molecular mechanisms act to regulate transcription of the genes within the lactose operon of E. coli . 2. Transcription of a eukaryotic protein-codi

Oestrous cycle, OESTROU S CYCLE - Also known as heat period. Common in...

OESTROU S CYCLE - Also known as heat period. Common in rabbit, cat, dog, sheeps. In this period desire for copulation occur. Possibility of fertilization is 100%.

Factors affecting normal blood sugar levels, Q. Factors Affecting Normal Bl...

Q. Factors Affecting Normal Blood Sugar Levels? Earlier in our discussion, we learnt that insulin is the most important factor responsible for normal blood sugar levels. Now we

Define liposuction - surgical management for obesity, Define Liposuction - ...

Define Liposuction - surgical management for obesity? Liposuction is a cosmetic surgical procedure different from bariatric surgery. It involves aspiration of subcutaneous fat

Explain adverse effects of ritonavir, Explain Adverse effects of ritonavir ...

Explain Adverse effects of ritonavir - Adverse reactions are common to full doses of ritonavir, but less common with the low doses used in PI combinations. Ritonavir can cause

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd