How do you verify that a certain gene encodes a certain prot, Biology

Assignment Help:
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains the gene encoding the protein. The last part of the question is where I am stuck which says: how would you verify that one of the genes you isolated does indeed encode a protein with the catalytic properties of the protein being studied?

Can someone please give me a good, detailed explanation as to an experiment I could do to verify that the gene I cloned encodes the protein I am interested in?

Related Discussions:- How do you verify that a certain gene encodes a certain prot

Oxidation of enzymes action, Oxidation Some enzymes which have the sulf...

Oxidation Some enzymes which have the sulfhydryl (-SH)  group in  the catalytic site are very sensitive to oxidation. Due  to oxidation of  the  -SH  group  by  aerial  oxygen

Describe anthracycline cardiomyopathy, Q. Describe Anthracycline Cardiomyop...

Q. Describe Anthracycline Cardiomyopathy? It is due to anticancer agent doxorubion (Adriamycine), when the cumulative dose exceeds 450 mg/m 2 in subjects with normal hearts. I

What are functions of the epithelium, Q. What are some functions of the epi...

Q. What are some functions of the epithelium? The epithelial tissues can perform protection, covering and impermeability against the environment, for instance, in the skin, res

Chemical energy to be used by the living beings on earth, Q. How is the lig...

Q. How is the light from the sun transformed into chemical energy to be used by the living beings on earth? Light from the sun is converting into chemical energy contained in o

Linkage of health with development, Linkage of Health With Development ...

Linkage of Health With Development Health, in its comprehensive or holistic sense, refers to the state of completephysical, mental, social and spiritual well being of a person.

Deficiency diseases-zinc deficiency, Zinc deficiency Zinc is essential ...

Zinc deficiency Zinc is essential for several physiological functions in the body such as protein synthesis, carbohydrate and nucleic acid metabolism, and foetal growth. The de

Biology, a_molecule can be dissolved in water solution but a _molecule does...

a_molecule can be dissolved in water solution but a _molecule does not mix with water

Secondary and tertiary consumers - biotic components, Secondary and Tertiar...

Secondary and Tertiary Consumers - Biotic Components Besides there are animals which depend on herbivores for food and are called secondary consumers. Since secondary consumer

Difference between "a" and "i" bands, DIFFERENCE S BETWEEN "A" AND "I" BAN...

DIFFERENCE S BETWEEN "A" AND "I" BANDS     A-Ban d (Anisotropic band)   I-Ban d (Isotropic band) 1. It

Explain cardiac prophylactic antibiotics, Cardiac Prophylactic antibiot...

Cardiac Prophylactic antibiotics can decrease the incidence of infection after cardiac surgery, and intraoperative redosing has been associated with adecreased risk of postoper

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd