How do you verify that a certain gene encodes a certain prot, Biology

Assignment Help:
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains the gene encoding the protein. The last part of the question is where I am stuck which says: how would you verify that one of the genes you isolated does indeed encode a protein with the catalytic properties of the protein being studied?

Can someone please give me a good, detailed explanation as to an experiment I could do to verify that the gene I cloned encodes the protein I am interested in?

Related Discussions:- How do you verify that a certain gene encodes a certain prot

Types of stem cells-pulp tissue revascularization, Embryonic stem cells: c...

Embryonic stem cells: can differentiate into all cell types of the specialized embryonic tissues when given sufficient and necessary stimulation for a specific cell type, e.g. Plu

Zoology, Give the salient features of phylum protozoa

Give the salient features of phylum protozoa

Two-kingdom classification, Two-Kingdom Classification The history of ...

Two-Kingdom Classification The history of taxonomy is as old as man himself. It may be traced back to prehistoric times because man had learnt to designate living organisms by

How does immune process that causes the hemolytic disease, How does the imm...

How does the immune process that causes the hemolytic disease of the newborn take place? In a hemolytic disease of the newborn the mother has Rh- blood and this mother when gen

Explain chiasms of homologous chromosomes, Q. What are the "chiasms" of hom...

Q. What are the "chiasms" of homologous chromosomes observe in prophase I? Chiasms are intersections of two tracts in the form of X, The chiasms seen in prophase I are chromoso

Whta is the effect of processing on mineral content of foods, Effect of pro...

Effect of processing on mineral content of foods Minerals are comparatively stable under processing conditions such as heat, light, use of oxidizing agents and extremes in pH.

Explain the micronutrients requirements during sepsis, Explain the Micronut...

Explain the Micronutrients Requirements during Sepsis Micronutrients: The requirement of almost all vitamins and certain mineral increases due to infection and inflammation. In

Digestive system - oesophagus, OESOPHAGU S - Gullet opens in oesoph...

OESOPHAGU S - Gullet opens in oesophagus. First organ of alimentary canal. After piercing diaphragm, it form stomach in abdominal cavity. No digestion in it. Food mov

When fecundation to be effective, Q. How long after ovulation must fecundat...

Q. How long after ovulation must fecundation occur to be effective? If fecundation doesn't occur around 24 hours after ovulation the released ovum often dies.

Explain precautions for gram staining of bacterial cultures, Explain Precau...

Explain Precautions for Gram Staining of Bacterial Cultures? 1. The dilutions should be chosen on the basis of expected counts in the food sample. 2. Food should be kept in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd