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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Q. What is a mixed gland? Why the pancreas is considered a mixed gland? Mixed gland is a gland that produces exocrine and endocrine secretions. The pancreas is an example of
Q. What are the parathyroids? Where are they located and what are the hormones secreted by these glands? The parathyroids are four small glands embedded two in each posterior f
Q. Guidelines for Diabetic Patients for Healthy Coping? Generally, a diabetic patient adapts to day to day stress effectively, as they accept their disease condition with the s
Q. Monounsaturated fatty acids required for dyslipidemia? Polyunsaturated fatty acids also have a cholesterol decreasing effect. Omega 3 fatty acids are excellent for the heart
Q. How many ATP molecules are made after the aerobic respiration and what is the net energetic gain of the process? After aerobic respiration 38 ATP molecules are made with the
Temperature - Seed Dormancy Low temperature treatment is an essential prelude to germination in many seeds, and high temperature may be inhibitory at the time of germination.
What is the fundamental difference between protozoans and algae? The basic difference among protozoans and algae is the fact that protozoans are heterotrophs whereas algae are p
Do we know what causes mental illnesses, or how best to treat them? Mental illnesses take various forms: debilitating sadness in depression; uncontrollable repetitive actions i
Unsaturated fatty acids require some additional processing before they can be degraded completely by β-oxidation. The Unsaturated fatty acyl CoAs with double bonds at odd-numbered
1. In chickens rose comb ( R ) is dominant over single comb ( r ). A rose-combed male is mated to a rose-combed female. Eighteen chicks are produced, ten of which are rose-combed,
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