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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
You have two enzymes, 1 and 2. Both convert substance A into substance B. 1 is inhibited by B, because B partially blocks the active site and will not allow more A to enter. 2 is i
PERSONNEL It is important that while allocating nursing, medical and paramedical staffs, the needs of the neonatal unit should not be ignored. The highest priority in the orga
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What factors lead to spoilage of fish flesh? A difference in the composition of tissues among different species, climate, procurement and holding practices are amongst few of
What is the Determination of vitamin B 1 Vitamin B 1 is determined by chemical method and microbiological assay. In the chemical method, vitamin B 1 is oxidized in alkaline
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When William was helping victims following a devastating earthquake in a region that was not prepared to swiftly set up adequate temporary shelter, he developed severe diarrhea. He
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