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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Q. How do biodiversity, the total number of living beings and the biomass respectively vary during the ecological succession? The Biodiversity, the biomass of an ecosystem and
Q. Is the esophagus a muscular organ? Why even in a patient lying totally flat on a hospital bed can the swallowed food reach the stomach? The esophagus is a predominantly musc
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Coding sequence is the portion of the gene or the mRNA which actually codes for a protein. Introns are not coding for the sequences; nor are the 5' or 3' untranslated regions (or
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Examination is begun by placing the transducer in left parasternal region, usually in the third or fourth left intercostal space. From this position, a sector image of the heart
characteristics of leucosolenia?
Dracunculiasis (guineaworm infestation) Dracunculiasis, a disease of man, which has been known since antiquity, is caused by the nematode parasite Dracunculus medinesis. The p
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