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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Transgenic Animal Over the centuries, animals with new combinations of genes have been produced using conventional breeding methods by means of careful selection of particular
iwant to know all about phylem protozoa?
Degree of dispersion of protein solution is decreased. It is decreased because of certain reasons:- Association: refers to changes occuring at subunit or molecular leve
This constitutes about 2.4% of the body weight of man viz. Brain-1400 gram Spinal nerve-150 gram Spinal cord-30 gram Cranial nerve-10 gram C.S.F. is altogether not present in
Define classification of carbohydrates - Disaccharides? Disaccharides are condensation products of two monosaccharide units joined together by a linkage called glycosidic linka
Define drug effects on food intake - cause dry mouth? cause dry mouth (xerostomia) : Lack of saliva makes it difficult to masticate and swallow foods, especially those of a dry
Explain the senses taste and smell ? The pigmented cone cells of the retina transmit information to bipolar nerve cells, then to ganglion cells, and on to the optic nerve and t
What is eutrophication? Eutrophication is the process of excessive enhancing of nutrients, like phosphate and nitrate, in water because of direct deposit of non treated sewage.
In 1798, British economist and demographer Thomas Robert Malthus, published his' essay on population' in which he stated that: 1. Population increases in a geometric rat
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