Freeze fracture electron microscopy, Biology

Assignment Help:

Freeze Fracture Electron Microscopy

Freeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).

1017_freeze microscopy.jpg


The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.


Related Discussions:- Freeze fracture electron microscopy

Impulse in the sensory neurone, Explain briefly how the stimulus at the fin...

Explain briefly how the stimulus at the finger produces an impulse in the sensory neurone using the following key words. 1. Energy conversion (linked to receptor) 2. Na+ in 3. Depo

Define field test used to assess aerobic capacity, Define Field Test used t...

Define Field Test used to Assess Aerobic Capacity? A field test that has been used extensively in nutrition studies to assess aerobic capacity measures Heart Rate responses to

Cerebrum, what term is used to indicate its grooves?

what term is used to indicate its grooves?

One benefit of including vegetable fibre in the diet, State one benefit of ...

State one benefit of including vegetable fibre (roughage) in the diet   Vegetable fibre retains water (keeping the faeces soft and bulky), stops constipation, decreases the

Modes of respiration, Modes of Respiration Many small organisms obtain...

Modes of Respiration Many small organisms obtain oxygen by diffusion through their body surfaces. They do not have any specialised respiratory organs nor do they have blood ci

Animal tissue culture , Animal Tissue Culture: The term tissue culture ...

Animal Tissue Culture: The term tissue culture refers to the culture of all organs, tissue fragments and dispersed cells on a suitable nutrient medium. It may be divided into

Explain glucose molecule runs the krebs cycle twice, Why can it be said tha...

Why can it be said that each glucose molecule runs the Krebs cycle twice? Every glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has g

Major objectives of diarrhoea, Q. Major objectives of diarrhoea? The ma...

Q. Major objectives of diarrhoea? The major objectives in the management of diarrhoea include: 1. Fluid and electrolyte replacement 2. Removal of cause (especially if inf

Explain materials for root end filling- amalgam, Explain Materials for Root...

Explain Materials for Root End Filling- Amalgam a) Its radiopacity is the better than any other REF materials b) Greatest amount of leakage! c) Corrosion, and significant

Liver function test, Liver function test should be done for all diabetic pa...

Liver function test should be done for all diabetic patients. This will help the doctor in the choice of drugs for the treatment of diabetes since liver abnormalities influence the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd