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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Q. What is a mixed gland? Why the pancreas is considered a mixed gland? Mixed gland is a gland that produces exocrine and endocrine secretions. The pancreas is an example of
what are genetics
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Dicots are one of the two main types of flowering plants; characterized by having two cotyledons, floral organs arranged in the cycles of four or five, and leaves with the reticul
What is Muscular dystrophy Muscular dystrophy :- Defects in the muscle due to faulty nutrition.
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What is the Vacuum Puff Drying? The vacuum puff process has been developed for drying liquids under vacuum. The development of processes for drying liquids under vacuum came fr
Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s
What should be the minimal distance between the implants or an implant and a natural tooth. The minimal distance between the implant and the adjacent tooth should be 1.5 -2 mm
Name the one autosomal dominant and autosomal recessive Mendel an disorder in Humans. a) How is the action of exonucluease dissimilar from that of end nuclease? b) India has
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