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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Elderly Is the treatment of hypertension different in elderly? Generally, the target for treatment should be at lower levels in the elderly. This is because, the elderly have
The NYQUIST LIIMIT-It is a sampling phenomenon, which limits the maximum fiequemcy shift measurement to one half of the sampling frequency (PRF). By its nature, pulse wave
Ask question importance of genetics in human life #Minimum 100 words accepted#
How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?
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Explain the Direct Surface Agar Plating Method? In the direct surface agar plating method, molten agar is placed on to the surface to be assessed. Upon hardening, it is incubat
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It is found in around 20 per cent cases of ARF and it is a late manifestation occurring even 3 months after GAS pharyngeal infection. Chorea is triggered by emotional disturbances
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