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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
An insect collecting net A useful insect net can be made from a round stick such as a broom or mop handle, some heavy wire and mosquito netting or cheese cloth. Bend a heavy pi
Red-green colour blindness is X-linked recessive. A woman with normal colour vision has a father who is red-green colour-blind. The woman has four sons, none of whom are colour-bli
Q. What is the notochord? How is this structure formed? The notochord is a rodlike structure that outlines the supporting axis of the embryo and gives birth to the vertebral co
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Sub Xyphoid Pericardial Window : It could be done under local or general anaesthesia. A small vertical midline incision is made over the xyphoid process on to the linea alba
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Name the various suturing techniques There are a various suturing techniques each suited for a particular situation. Few of the common suturing techniques are mentioned here
Q. Signs and Symptoms diabetic ketoacidosis? The following are the signs and symptoms of DKA: 1) Increased heart rate (tachycardia) 2) Low blood pressure 3) Dehydratio
Vasoactive substances such as Endothelium Derived Relaxing Factors (EDRF). Prostacyclin and Endothelin can be formed in the vascular endothelium. Endothelial dysfunction can lead t
Explain Positive Staining Technique? Here, a stain has a positively charged chromophore that gets attached to the negatively charged outer surface of the microbial cell and thu
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