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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Medical Nutrition Therapy Medical Nutrition Therapy (MNT) is defined as the assessment of the nutritional status of a client followed by nutrition therapy ranging Pon
Explain Intensity of heart sound? Intensity of S 1, particularly M 1 depends on the position of the bellies of mitral leafets when the LV begins to contract since S 1 is loudes
Brooke's Formula: a) Fluid requirement b) Estimate the accurate/approximate weight of the patient c) First 24 hours Colloids (blood, plasma, dextran) 0.5 ml
Syngamy - Patterns of Sexual Reproduction Sperm fuses with the egg. This results in both the union of the paternal nucleus with the maternal one (karyogamy), as well as the fu
Q. What is mitral stenosis? Most common cause of mitral stenosis is rheumatic fever. Nearly 30 per cent of patients with rheumatic fever may go on to develop pure mitral valve
Define Nutritional Management of Food Allergies and Food intolerance? In this unit we will be discussing the adverse reactions associated with food. You may have read or heard
type study of polystomella
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What are the hormones secreted by the neurohypophysis? What are their respective functions? The neurohypophysis secretes oxytocin and the antidiuretic hormone (ADH). Oxytoci
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