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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Aplasia of epidydimis This condition is seen in only 1 or 2 % of young bulls and is marked by absence of one (more common) or both epididymis. Bulls having a single epidydimis
Why do cells of the nephron tubules present a great amount of mitochondria? The cells of the tubule wall have high number of mitochondria because lots of substances are resorbe
Define Interactions between Volatile Substances and Proteins? Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffusion
The mirrows reach or grayling zone This zone has a less steep gradient, than the trout beck. The river here still flows swiftly, though the current is not fast and conditions
discuss chromosomal theory of heredity.
Explain Properties of Natural fats and oils Natural fats and oils vary widely in their physical properties even though they are composed of the same or similar fatty acids. Th
EXAMPLES OF NATURAL SELECTION - 1 . INDUSTRIAL MELANISM - First studied by Fischer & Ford (1845) on pepperd moth (Biston betularia) of two types : grey moth (B.
what is life process
Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea
Organisms are made to compete for their needs from the environment. The competition as we pointed earlier could be for the food and territory, to overcome the adverse climatic cond
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