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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Vesicular stomatitis Vesicular stomatitis (VS) is an infectious viral disease of horses, cattle and pigs caused by Vesiculovirus of the family Rhabdoviridae and characterized by f
#questionThree actographs (look closely to see the labels A, B, and C). Assume that the abscissa is either 24 or 48 hours for each actograph. You are asked to write two sentences f
Give an example of each of the following types of proteins: a. Enzyme b. Structural protein c. Motor protein
coxa of paleomon contain specialised organ called
Explain Pennate diatomsrole in diatoms? Pennate diatoms have bilateral symmetry, which presents side-by-side mirror images if divided down the middle or centerline, as seen
Why is it important that a cell membrane does not allow all dissolved substances to diffuse freely through it? If the cell membrane were freely permeable, harmful substances co
Aortic Valve Replacement with Replacement of Aortic Root : When there is aneurysm of ascending aorta with aortic regurgitation, aortic root replacement is done. This
Medication The patient is advised to continue antibiotics for a week more after discharge. Other medications prescribed are analgesics (acetaminopen), cough syrup, diuretics
Determine Causes of Malnutrition? You may recall studying about the causes of malnutrition earlier. The causes of malnutrition are classified as immediate (individual level), u
Techniques : Depending on the type of surgery planned, the heart may be approached through median sternotomy, left or right thoracotomy. The problems encountered could be due to
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