Freeze fracture electron microscopy, Biology

Assignment Help:

Freeze Fracture Electron Microscopy

Freeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).

1017_freeze microscopy.jpg


The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.


Related Discussions:- Freeze fracture electron microscopy

Write short note on cholesterol, Cholesterol, from stereos (solid) and the...

Cholesterol, from stereos (solid) and the Greek chole- (bile) followed by the chemical suffix -ol for an alcohol is an organic chemical substance classified as a waxy steroid of fa

Reproduction, write about asexual reproduction in mucor?

write about asexual reproduction in mucor?

Determine the wechsler intelligence scales, Determine the Wechsler Intellig...

Determine the Wechsler Intelligence Scales This test is given according to manual instructions and is not modified in any way. Most clinicians use the most current revision of

Define single beam and double beam optical path, Define Single Beam and Dou...

Define Single Beam and Double Beam Optical Path? In simpler instruments, a single beam of light passes through the cuvettes and reaches the detector whereas in the double beam

Describe the stages of atherosclerosis, Describe the Stages of Atherosclero...

Describe the Stages of Atherosclerosis ? The process of atherosclerosis which gives rise to CAD marches in stages. The earliest recognizable pathologic lesions are the fatty st

What do you understand by arthropodization, What do you understand by Arthr...

What do you understand by Arthropodization? Many of the traits that we consider unique to the Arthropoda may not be independent traits. Instead, they may be the consequence of

Explain the complexity level of the nervous system, Q Is the tubular-dorsal...

Q Is the tubular-dorsal nervous system of chordates associated to radial or lateral symmetry? How does that explain the complexity level of the nervous system reached by the verteb

Define extra enterocyte transfer - non haem iron absorption, Define Extra e...

Define Extra enterocyte transfer - non haem iron absorption? Extra enterocyte transfer: Little is known about iron transport across the baso- lateral membrane. After crossing t

What are the inheritance and dominance patterns of rh blood, What are the i...

What are the inheritance and dominance patterns of the Rh blood system? An inheritance pattern of the Rh blood system is autosomal dominant that is the heterozygous manifests a

Explain the generalities, Explain the Generalities? A binary system has...

Explain the Generalities? A binary system has two components; C equals 2, and the number of degrees of freedom is F = 4 - P. There must be at least one phase, so the maximum po

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd