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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Epimerism:- Another type of stereo isomerism known as epimerism occurs with respect to a single asymmetric carbon atom of a monosaccharide possessing more than one asymmetric
Q. Explain about Long acting insulin? Long acting: Long acting insulin does not work until 4 Lo 8 hours after injecting. Its peak activity occurs 18 to 24 hours after injectio
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The following histograms were produced using flow cytometry after labelling B-cell lymphocytes with propidium iodide. Histogram A is from a healthy individual while Histogram B is
Injection Route An injection is an unpleasant experience for a person at any age and is particularly traumatic to the small child who often lives in fear to the next "need
Define Procedure for Detection of Number of Bacteria in Milk? 1. Place a clean, non-greasy slide over the graph paper. 2. Spread 0.01 ml of the milk over an area of 1 cm2 as
Interactions between minerals Copper deficiency has been identified as a serious problem for grazing ruminants. A deficiency may be due to low concentrations of Cu in forage a
The main objectives of study of ecology are: (i) To study the distribution of biota local and geographical level. (ii) To study the interrelationship
Explain Relative Quantitative difference of Protein Taxonomy This analysis involves the study between the amounts of different proteins and more significantly, of different con
DIFFERENCE BETWEEN VEGETATIVE AND GENERATIVE CELL
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