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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Food applications of CMC In common with other linear water-soluble polymers, cellulose gum solutions are pseudoplastic that is the viscosity decreases as the rate of shear inc
Q. Dietary management during atherosclerosis? Dietary management and the nutrient requirements during atherosclerosis remain the same as for the management of dyslipidemia. Hen
Properties of Stem Cells Stem cells are defined by having 3 major properties: 1) They are capable of self-renewal. 2) They divide, some daughter cells give rise to cells
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Explain the steps of converting food into energy. There are four steps to convert food into energy: a) Ingestion, mastication and swallowing : Ingestion or taking in of food
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Can mitosis occur in haploid (n) cells? And in triploid cells? The mitotic cell division can happen in haploid (n) cells, diploid (2n) cells, triploid (3n) cells, etc. Mitosis
Q. Do plants present only sexual reproduction? There are asexual forms of reproduction in plants few naturally detached pieces of root, leaves or limbs develop into another com
Osmoregulation in Protozoans Osmoregulation or water balance in protozoa is accomplished by contractile vacuoles. One to several contractile vacuoles may be present within the
Evolution of migration Eg Benefits associated with migratory flights must be greater than those associated with staying in Alaska and having to tolerate seasonal changes
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