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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Q. How different are gymnosperms from pteridophytes and bryophytes? Gymnosperms are not cryptogamic as pteridophytes and bryophytes are. They are phanerogamic and so they form
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Domestication - Wildlife It means that man has taken under his direct care the living beings which are useful to him. Through extensive breeding programmes, he has modified th
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Epidemiological Theory - Accident Causation Epidemiological theory of accident causation considers industrial hygiene that concerns environmental issues and may result in sick
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Q. Define characteristic of Doppler Echocardiographic? Ans. Doppler findings of cardiac tarnponade are based on the following characteristic respiratory variations in intr
Protozoa general characters and classification
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