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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
What is the most accepted hypothesis about the origin of life on earth? How does it compare to the other main hypotheses? The heterotrophic hypothesis is the strongest and most
Grasping instruments -Plier or clamp forceps -put piece of rubber in the plier -applied on the buccal and lingual surface -make pressure to
What is Large Intestine in human biology? Food undigested in the small intestine is passed on by peristalsis into the large intestine, or colon. The intestine is composed of ci
In some protozoans, food is always ingested at the same place on cell surface, cytostome (cell mouth). Undigested food is removed at a cytoproct, or cell anus. All of this is simil
Which of the following is true in a skeletal muscle? A. Movement of the cross-bridge occurs only when the myosin head is detached from the actin molecule. B. The binding of
Q. What are pioneer species? What is the role of the pioneer species? The Pioneer species are those first species that colonize places where previously there were no living bei
Dystocia Dystocia or difficult calving is a condition where help is required as providing traction, repositioning of fetus, foetotomy or caesarotomy. Generally, ease of calvin
Define measuring body composition- Skin fold measurement? The clinical use, skin fold measurement can be taken with the help of calipers. The calipers are applied at constant p
Biochemistry : The chemistry of living organisms that covers all the chemical reactions occurring in our body.
Many of the free energy free during the oxidation of glucose to CO 2 is retained in the decreasing coenzymes NADH and FADH 2 producing while glycolysis and the citric acid cycle.
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