Formation of  lactate and  its consumption

Formation of  lactate and  its consumption: If anaerobic conctitions prevail, the reoxidation  of NADH  through the respiratory chain  is  prevented. Then the regeneration  of NAD'  from NADH  is  carried out by  the  action  of  lactate dehydrogenase  on pyruvclte resulting  in lactate  and NAD' thereby allowing the glycolytic cycle to continue even in the absence of oxygen. Thus tissues that function under hypoxic conditions  tend to produce lactate. The advantage of using pyruvate  for  reoxidation ofNADH  lies  in the fact that pyruvate iis  the end product of glycnlysis  and would readily be available in the cells.