Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Define tests for the presence of exoenzymatic activity, Define Tests for th...

Define Tests for the Presence of Exoenzymatic Activity? Microorganisms require various micro- and macro-nutrients for energy production and growth. These are obtained from the

Why is quarrantine introduction of a plant species, Why is quarrantine a mu...

Why is quarrantine a must before introduction of a plant species from a dissimilar country? a) What is meant by apoplast pathway? Why does it occur in cortex and not in endoder

Explain pro-oxidants - lipid oxidation, Explain Pro-Oxidants Transitio...

Explain Pro-Oxidants Transition metals, particularly those possessing two or more valency states and a suitable oxidation - reduction potential between them (e.g., cobalt, cop

Is it necessary for blood to have less or more hemoglobin, Q. In high altit...

Q. In high altitudes is it necessary for the blood to have less or more hemoglobin? In high altitudes the oxygen concentration and air is rarefied is lower than in low altitude

Define preoperative care prior to endodontic microsurgery, Define Preoperat...

Define Preoperative Care Prior to Endodontic Microsurgery a.Thorough medical history "consultation and permission from the physician is the patient has systemic disease(s) and

Coelomoducts in polyplacophora, Coelomoducts in Polyplacophora In Poly...

Coelomoducts in Polyplacophora In Polyplacophora the coelomoducts divide in the region of coelomostome and the gonadal cavities become closed off from pericardial coelom. The

Explain how a cell produces and releases proteins, Explain how a cell produ...

Explain how a cell produces and releases proteins. Proteins are made on ribosomes and packaged into vesicles by the Golgi apparatus. The vesicles move to the cell membrane and

Excretion in amoeba, EXCRETIO N IN AMOEBA - NH 3  is excreted out t...

EXCRETIO N IN AMOEBA - NH 3  is excreted out through plasmalemma. Osmoregulation takes place by contractile vacoule, generally one, towards posterior end, contractile in

Difference between dominant epistasis and recessive epistasi, What is epist...

What is epistasis? What is the difference between dominant epistasis and recessive epistasis? The Epistasis is the gene interaction in which a gene (the epistatic gene) able to

Explain the glass test tubes - food microbiology, Explain the Glass Test Tu...

Explain the Glass Test Tubes - Food Microbiology Sterile glass tubes are used for culturing microorganisms in liquid or solid media. Sterility in tubes is maintained by using c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd