Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Phylum coelenterata, #questioen..what is the example of outline og phylum c...

#questioen..what is the example of outline og phylum coelenterata

Is there a respiratory pigment in the annelid blood, Is there a respiratory...

Is there a respiratory pigment in the annelid blood? The blood in beings of the phylum Annelida have the respiratory pigment hemoglobin (the similar found in chordates) and oth

Xerarch - kinds of succession, Xerarch - Kinds of Succession When the ...

Xerarch - Kinds of Succession When the succession takes place in drier area, i.e., the succession progresses from xeric to mesic conditions. It is further subdivided as:

Determine the architecture of cell, Determine the architecture of cell ...

Determine the architecture of cell There are over 200 types of cells in the human body, which are assembled into a variety of tissues, such as, the epithelia, connective tissue

Write the chain structure of d galactose and d mannose, Write the chain str...

Write the chain structure of D galactose and D mannose? The ring form of ribose is a component of ribonucleic acid (RNA). Deoxyribose, which is missing an oxygen at positi

Explain the small intestine, Explain the Small Intestine? The small int...

Explain the Small Intestine? The small intestine is made up of three sections, the duodenum, the jejunum, and the ileum. Bile from the liver and pancreatic enzymes are released

Neurochemistry of attention, Neurochemistry of Attention Different neur...

Neurochemistry of Attention Different neurotransmitters systems are implicated in attention. Cholinergic, dopaminergic and serotonergic systems play major roles in neurochemica

Precautions for determination of iodine number of lipids, Precautions for d...

Precautions for determination of iodine number of lipids? 1. If using the pipette for transferring the oil, allow the pipette to drain slowly and completely, as oil takes time

Define low water permeability across the luminal membranes, A healthy perso...

A healthy person is on a diet that having of very small amounts of water. A. This person will make a large volume of dilute urine with low amounts of dissolved solutes. B. T

Deer, How does a deer feed

How does a deer feed

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd