Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Totipotency and pluripotency, Totipotency and Pluripotency In the sta...

Totipotency and Pluripotency In the starting we said that the fertilized egg cell (zygote) has the capacity or potentiality to give rise to all kinds of cell types, like a bl

Understanding life, UNDERS T ANDIN G LIF E - Presence of proto...

UNDERS T ANDIN G LIF E - Presence of protoplasm is the important feature of life which acts the site of metabolism. Maintenance of life by protoplasm requires conti

Illustrate the major events of the final mitotic period, Q. What are the ma...

Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea

State three structural features of prokaryotic cells, Explain Following Tre...

Explain Following Trems (a) State three structural features of prokaryotic cells. (b) Describe the functions in eukaryotic cells of lysosomes, ribosomes, and centrioles.

Factors related to causing malnutrition among chd patients, Define Factors ...

Define Factors related to Causing Malnutrition among CHD Patients? The various factors related to causing malnutrition among CHD patients are hypoxia (reduction in oxygen suppl

Determine about the open head injuries, Open Head Injuries Open head in...

Open Head Injuries Open head injuries are TBIs in which the skull is penetrated, as in gun shot or missile wounds, or in which fragment of bone penetrate the brain substance. O

Define isomerases, Isomerases These  enzymes (code  EC 5)  catalyze geo...

Isomerases These  enzymes (code  EC 5)  catalyze geometric  or  structural  changes  within  a molecule. According  to the  type of  isomerism,  they  may be  called  racemases

Explain pancreatitis, Explain Pancreatitis Pancreatitis :- Inflammati...

Explain Pancreatitis Pancreatitis :- Inflammation of the pancreas.

What is the parasite that causes giardiasis, Q. What is the parasite that c...

Q. What is the parasite that causes giardiasis? How is it transmitted and what are the typical manifestations of the disease? The Giardiasis is a protozoal infection caused by

Biochemistry pathway of normal and cancer cell, Biochemistry pathway of nor...

Biochemistry pathway of normal and cancer cell Normal cells will separate within the presence of a chemical signal like as "growth factor" (GF). Other molecular signals

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd