Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

What are the major terrestrial biomes, What are the major terrestrial biome...

What are the major terrestrial biomes? The main terrestrial biomes are: tundra, taigas (or boreal forest), tropical forests, temperate forests, grasslands and deserts.

General characteristics of protozoans, General Characteristics of Protozoan...

General Characteristics of Protozoans The protozoans, are eukaryotes that form a group of about 80,000 single-celled organisms under the kingdom Protista. The unicellular leve

Xerarch - ecology, Xerarch - Ecology Successions initiated on bare roc...

Xerarch - Ecology Successions initiated on bare rock, wind-blown sand, rocky talus slopes, or other situations where there is an extreme deficiency of water are termed xerarch

Explain the effect of deficiency of pyridoxine, Explain the effect of Defic...

Explain the effect of Deficiency of pyridoxine? A deficiency of vitamin B6 alone is uncommon because it usually occurs in association with a deficit in other B-complex vitamin

Amphibians, respiratory system in amphibains

respiratory system in amphibains

Cleavage, CLE A V AG E - Holoblastic & unequal. First plane is meri...

CLE A V AG E - Holoblastic & unequal. First plane is meridional. 2 blastomeres are formed. 1 megamere & another micromere. 2nd plane is also meridional but at 90° to fir

Regulation of egg, difference between mosaic and regulation egg

difference between mosaic and regulation egg

Why should an athletes take proteins, Why should an athletes take Proteins?...

Why should an athletes take Proteins? Proteins are basically taken for their ergogenic properties of enhancing endurance and increasing or maintaining muscle mass to improve st

Define major structures of chloroplasts, Q. What are the major structures o...

Q. What are the major structures of chloroplasts? Chloroplasts are involved by two membrane layers the inner and the outer membranes. Inside the organelle the formative unit is

What is phytates, Q. What is Phytates? Phytates are widely distributed...

Q. What is Phytates? Phytates are widely distributed in seeds of vegetables, fruits and grains. Unrefined cereals and millets are the richest sources of phytates. Refined or

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd