Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Define transverse section of body of male ascaris, Define Transverse sectio...

Define Transverse section of body of male Ascaris? 1. Body wall consists of the following: (a) Cuticle is the outermost layer. It is a thick, tough, elastic membrane

Bovine ephemeral fever, Bovine ephemeral fever Ephemeral fever is commonly...

Bovine ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquito-borne vir

Nutritional demands of sports and dietary recommendations, Define Nutrition...

Define Nutritional Demands of Sports and Dietary Recommendations? There is a strong relationship between nutritional status and physical training. Whether it is to maintain hea

Define cerebral hemisphere tissue, Q. Define cerebral hemisphere tissue? ...

Q. Define cerebral hemisphere tissue? CEREBRAL WHITE MATTER - The remaining cerebral hemisphere tissue is the deeper cerebral white matter (60% of cerebral volume) which includ

What is the difference between taeniasis and cysticercosis, What is the dif...

What is the difference between taeniasis and cysticercosis? Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. Cysticercos

Point out some guidelines for breast-feeding, Point out some Guidelines for...

Point out some Guidelines for breast-feeding? 1) Follow demand feeding of the baby instead of a fixed schedule. 2) Initiate breast-feeding within 2 h after a normal delivery

Drinking & driving, DRINKING & DRIVING - It should not be mixed because...

DRINKING & DRIVING - It should not be mixed because alcohol - 1.       Impairs ability to judge distances. 2.       Reduces capacity to coordinate limb muscles. 3.

Explain glomerular filtration in human nephrons, A horticulturist keeps Chr...

A horticulturist keeps Chrysanthemum plants in short-day conditions during long-day season. State its effect on flowering. Specify the role of phytochrome included. Explain glom

Differentiate between sterilization and disinfection, Q. Differentiate betw...

Q. Differentiate between sterilization and disinfection? Sterilization is defined as freeing the object or substance from all life of any kind. It is the process by which an ar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd