Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Show the types of oesophagitis conditions, Q. Show the types of oesophagiti...

Q. Show the types of oesophagitis conditions? The two types of oesophagitis conditions: 1. Acute Oesophagitis - It is characterized by substantial pain on swallowing. It is

Digestive system - salivary glands, SALI V A R Y GLAND - These are ...

SALI V A R Y GLAND - These are compound - Alveolar, tubular glands made up of columnar / cuboidal epithelium. Saliva is manufactured in them. In mammals these are pai

Causes of hypoglycaemia, Since hypoglycaemia can occur in any diabetic pati...

Since hypoglycaemia can occur in any diabetic patient at any time, it is important for you to learn the causes of hypoglycaemia. Such knowledge will help you to educate the patient

Poultry and duck diseases-hydropericardium syndrome, Hydropericardium syndr...

Hydropericardium syndrome Hydropericardium syndrome (HPS), known as Leechi disease, is caused by Avian Adenovirus 4. HPS has been responsible for high mortality ranging from 6

Weaning the infant from the incubator, Weaning the Infant from the Incuba...

Weaning the Infant from the Incubator When heater output reading is minimal or nil it suggest that infant is capable of generating enough metabolic heat to keep himself war

Draw a diagram that shows this arrangement, Please help with the following:...

Please help with the following: Using the letters S for ribose sugar and B for the nitrogeneus base, draw a diagram that shows the arrangement of these components into (a) a sin

Procedures for diagnosis - isolation of etiological agent, Isolation and id...

Isolation and identification of the etiological agent: Isolation of the etiological agent is attempted in chicken embryos, cell cultures, laboratory animals, culture medi

What is the procedure of uristix, What is the Procedure of Uristix 1. C...

What is the Procedure of Uristix 1. Collect the urine. 2. Remove one strip from bottle and replace cap. 3. Completely immerse reagent areas of the strip in urine and remo

Define food sources of calcium, Define Food Sources of Calcium? Dairy p...

Define Food Sources of Calcium? Dairy products are of course the primary source of calcium followed by grains and pulses. Among the millets, ragi contains substantial amount of

Complementation of genes, how would one determine that the wild type phenot...

how would one determine that the wild type phenotype of a certain trait is due to the action of one or two genes?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd