Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Pyruvate carboxylase activation, Oxaloacetate has two main roles. It is an ...

Oxaloacetate has two main roles. It is an intermediate which is consumed in gluconeogenesis and it is also a key intermediate in the citric acid cycle where it fuses with acetyl Co

What is the name of process of deposition, Thickness and toughness of plant...

Thickness and toughness of plant walls are increased by deposition within original cellulose wall, of a substance called as lignin. Process of deposition is called: a) Lignific

Plant classfication, why obelia is considered to be of special interest in ...

why obelia is considered to be of special interest in zoology as an animal showing an intermediate grade of organisation

Evaluation of nursing process, Evaluation This is the last step in nur...

Evaluation This is the last step in nursing process. Throughout care, you constantly evaluate the progress the client has made towards reaching the pre-established goals/ ob

Give the details of colour vision, Give the Details of Colour Vision Th...

Give the Details of Colour Vision The genes for colour sense are located on the X chromosome (recessive inheritance) and on chromosomes 3 and 7. Changes in the cone pigments as

Gel shift assay, Gel shift assay is a technique by which one can determine...

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiol

How is the body of gastropods divided, Q. How is the body of gastropods div...

Q. How is the body of gastropods divided? The body of gastropods is divided into three major portions: head, foot and the visceral mass. Q. What is the type of digestive sy

Determine majority of segregation errors in human patients, Which of the fo...

Which of the following defines why the vast majority of segregation errors in human patients include the two sex chromosomes (XXY, XO, XYY) or chromosome 21 (triple 21)? A. The

Viruses, what is the basic structure of a virus?

what is the basic structure of a virus?

What are phospholipids, What are phospholipids? Phospholipids are molec...

What are phospholipids? Phospholipids are molecules made of glycerol bound to two long molecules of fatty acids and to one phosphate group. Thus, phospholipids are amphipathic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd