Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Buffalo-pox, B u f fa l o- p o x The disease i...

B u f fa l o- p o x The disease is caused by an orthopox virus, closely related to the vaccinia virus. It is not clear whether it should be considered

Explain about the rna viruses - carcinogenic, Explain about the RNA Viruses...

Explain about the RNA Viruses - carcinogenic? All oncogenic RNA viruses are retroviruses. They are of 2 types. They are acute transforming retroviruses and slow transforming re

Modern biochemistry and cell physiology, This problem set is a little diffe...

This problem set is a little different, in that it is one large project/problem. To answer it, you must write a short single-spaced typed report of 4-5 pages MAXIMUM. Here is the p

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

Explain the class reptilia perform gas, Do beings of the class Reptilia per...

Do beings of the class Reptilia perform gas exchange in the same way amphibians do? These beings do not have permeable skin so they do not make cutaneous respiration such as a

Myocardial energetics, The oxidation of substrates like free fatty acids (F...

The oxidation of substrates like free fatty acids (FFA), glucose, lactate and ketone bodies results in the energy of Adenosine triphosphate (ATP) and Creatine phosphate (CP). The s

Medication therapy and nursing consideration, Mr. Smith is a 72 year male d...

Mr. Smith is a 72 year male diagnosed with hypertension.  Along with hypertension, Mr. Smith has been diagnosed with right-sided heart failure.  The following are his list of medic

Subdivisions of ecology, Subdivisions of Ecology Ecology was earlier di...

Subdivisions of Ecology Ecology was earlier divided into plant and animal ecology. However, modem ecology does not make any such distinction since plants and animals are intima

Explain noncompetitive inhibition, Noncompetitive inhibition In  this ...

Noncompetitive inhibition In  this  type  of  inhibition, the inhibitor binds at  a site  on  the  enzyme  other  than catalytic site. As there is no competition between the s

Gibberellins - plant growth substances, Gibberellins - Plant Growth Substan...

Gibberellins - Plant Growth Substances In late 1920's Kurosawa observed that the medium in which the fungus Gibberella had grown could cause the abnormal tallness in rice seed

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd