Sample titration - estimation of nitrogen & protein content, Biology

Assignment Help:

Define Sample Titration - Estimation of nitrogen and protein content?

Take the given ammonium sulfate solution into a 100 ml volumetric flask and dilute to the mark with distilled water. Mix well. Set up the steam distillation apparatus and pass steam through the apparatus for cleaning it. After the apparatus is cleaned, pipet 10 ml Tashiro's mixture into a 100 ml conical flask and place it at the delivery end of the apparatus making sure that the delivery tube is dipping in the solution. Add 10 ml of the dilute sample from the funnel in the apparatus and few drops of phenolphthalein.

After this wash the funnel with about 5 ml distilled water to ensure that all the ammonium sulphate has reached the distillation flask. Then add 10 ml of 40% NaOH and immediately close the stopper. Now heat the flask to pass the steam through this mixture. The liberated NH3 from ammonium sulphate is collected in the Tashiro's mixture in the receiving flask. Continue distillation until the level of the solution in the receiving flask becomes approximately double. Remove the receiving flask and rinse the tip of the delivery tube with distilled water. Titrate the solution in the flask against standard H2SO4 till the color changes from green to pink.


Related Discussions:- Sample titration - estimation of nitrogen & protein content

Therapeutic dose of pyridoxine, Q. Therapeutic dose of pyridoxine? Ther...

Q. Therapeutic dose of pyridoxine? Therapeutic dose of pyridoxine (lg/day) is usually tried in all patients with hypermethioninernia and homocystinemia. For patients who do not

Starch, S T ARC H - Polymer of a-D-glucose. Starch is glucosan...

S T ARC H - Polymer of a-D-glucose. Starch is glucosan homopolysaccharide which is the major reserve food of plants. Starch is formed as an end product of photosynt

What is genetic engineering, Q. What is genetic engineering? Genetic en...

Q. What is genetic engineering? Genetic engineering, It is also known as molecular cloning or gene cloning, is artificial recombination of nucleic acid molecules in a test tube

What are the joints, What are the joints Joints are the place of artic...

What are the joints Joints are the place of articulation between two or more bones, or between a bone and a cartilage. Due to the presence of a number of joints, the movement

How can we determine the status of brain function, How can we determine the...

How can we determine the status of brain function Neuropsychological assessment is the clinical practise of using tests and other behavioural evaluation instruments to determin

Which germ layer originate liver and the pancreas, Q. From which germ layer...

Q. From which germ layer do the liver and the pancreas originate? What are other organs and tissues made from that germ layer? The pancreas and the liver are originated from th

DEVELOPMENTAL BIOLOGY, DIFFERENCE BETWEEN VEGETATIVE AND GENERATIVE CELL

DIFFERENCE BETWEEN VEGETATIVE AND GENERATIVE CELL

How implant design and surface affect osseointegration, Q. How Implant desi...

Q. How Implant design and surface affect osseointegration? Implant design Most conductive design for osseointegration is root form. They can be threaded, hydroxyapatite co

#title.examples of classes of vertebrates, how to explain an example from e...

how to explain an example from each class of vertebrate

Results-types of surgery, Results: Early mortality for tricuspid valve re...

Results: Early mortality for tricuspid valve replacement is around six per cent. But depending on the patient's class of symptoms and the number of concomitant procedures, this c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd