Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Nuclear type - endosperm, Nuclear Type - Endosperm The primary endosp...

Nuclear Type - Endosperm The primary endosperm nucleus divides. The cell wall is not laid. These nuclei and their division products form a large number of free nuclei. The fi

State the branemark theory of osseointegration, Branemark's Theory of Osseo...

Branemark's Theory of Osseointegration In 1952, Swedish orthopedic surgeon, Branemark, conducted microscopic studies done in situ of the bone marrow in rabbit's fibula with in

How many weeks are required to recover after blood donation, When a human d...

When a human donor gives a pint of blood, it generally requires how many weeks for body RESERVE of red corpuscles to be replaced? Is it: a) 7 week b) 3 weeks c) 1 weeks

Determine the synaptic process, Since neurotransmitters are not consumed in...

Since neurotransmitters are not consumed in the synaptic process, what are the mechanisms to decrease their concentrations in the synaptic cleft after they have been used? As t

Vaccine against tuberculosis, Q. Is there vaccine against tuberculosis? ...

Q. Is there vaccine against tuberculosis? The vaccine against the tuberculosis is called as BCG (bacillus Calmette-Guérin). BCG is not used in a few countries where tuberculosi

Show characteristics of food products, Q. Show characteristics of food prod...

Q. Show characteristics of food products? These sensory characteristics of food products. Food quality, as such, can be divided into three main categories i.e. i. Appeara

Explain about the optic disc, Explain about the optic disc The optic di...

Explain about the optic disc The optic disc is also called a blind spot while the macula is referred to as the yellow spot. The ora serrata (the intersection between the retina

Circuitry and priming -procedures of oxygenators, Circuitry and Priming : ...

Circuitry and Priming : The cardio pulmonary bypass circuit consists of oxygenator, tubings, cannulae, cardiotomy reservoirs and cardioplegia attachments. The first step is to pri

What is isozymes, ISOZYMES : an enzyme having different molecular forms bu...

ISOZYMES : an enzyme having different molecular forms but catalyzing the same reaction.

Oral route - administration of drugs, Oral Route Paediatric Medication...

Oral Route Paediatric Medications are prepared as Tablets (which can be crushed), powders, granules, mixtures, emulsions, elixirs and syrups. It is easier to persuade olde

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd