Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Membrane protein purification and reconstitution, The first step of the pur...

The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane.  This is commonly ach

How hermaphrodite species present cross-fecundation, Is it possible for a h...

Is it possible for a hermaphrodite species to present cross-fecundation? There are hermaphrodite species of animals and plants that present cross-fecundation mainly because of

Excretory system, extensive note on the excretory system of amphibian

extensive note on the excretory system of amphibian

Explain the process of biosynthetic phase, Describe the process of biosynth...

Describe the process of biosynthetic phase of photosynthesis occurring in the chloroplast. Explain the process of development of root nodules in a leguminous plant. Name the ox

Respiration, how energy enters and then flows through a biological system

how energy enters and then flows through a biological system

Survival of the saddleback, The North Island saddleback or tieke, Philestur...

The North Island saddleback or tieke, Philesturnus rufusater, is an endemic New Zealand bird. Saddlebacks inhabit the middle or lower layers of the forest, and eat small invertebra

Psychoactive substance use disorders, PSYCHOACTIVE SUBSTANCE USE DISORDERS:...

PSYCHOACTIVE SUBSTANCE USE DISORDERS: Drug: It is derived from a French word 'drogue'. A medical substance used in the treatment of disease. (Taber's  Dictionary).  'Drug d

Define criteria for assessment of pyridoxine status, Define Criteria for As...

Define Criteria for Assessment of Pyridoxine Status? Vitamin B 6 status is most appropriately evaluated by using a combination of indicators,  namely plasma PLP concentration,

Constituents of cell walls in bacteria, Q. What are the main respective con...

Q. What are the main respective constituents of cell walls in bacteria, protists, fungi and plants? In bacteria the cell wall is made of peptidoglycans, amongst protists algae

Applications of genetic engineering, Q. Applications of genetic engineering...

Q. Applications of genetic engineering? Applications of genetic engineering in different fields are: • Agriculture: Crops having larger yields, drought -and disease -resis

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd