Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

What are the amylase inhibitors, What are the Amylase Inhibitors? Inhib...

What are the Amylase Inhibitors? Inhibitors of the enzyme a-amylase are found in wheat, rye, beans, mango, legumes, potatoes, sorghum (jowar) and oats. Most amylase inhibitors

Atp molecules produced for each glucose molecule, Q. How many ATP molecules...

Q. How many ATP molecules are produced for every glucose molecule used in fermentation? And How many ATP molecules are produced for each glucose molecule used in aerobic respiratio

Cell membrane does not allow all dissolved substances, Why is it important ...

Why is it important that a cell membrane does not allow all dissolved substances to diffuse freely through it? If the cell membrane were freely permeable, harmful substances co

Explain the type I diabetes mellitus, What the difference is between type I...

What the difference is between type I diabetes mellitus and type II diabetes mellitus? Type I diabetes, also called as juvenile diabetes, or insulin-dependent diabetes (this na

Energy budget - ecosystem, Energy Budget - Ecosystem All living things...

Energy Budget - Ecosystem All living things must take in and use energy to maintain their bodies, to grow, to obtain more energy and to reproduce. Each individual has an 'ener

What is a skiers launch speed, In the aerials competition in skiing, the co...

In the aerials competition in skiing, the competitors speed down a ramp that slopes sharply upward at the end. The sharp upward slope launches them into the air, where they perform

Define food availability and food access, Define Food availability and Food...

Define Food availability and Food Access? Food availability: This refers to availability of necessary types of food in sufficient quantity, to the individual. The sources may b

Do protozoans have asexual or sexual reproduction, Q. Do protozoans have as...

Q. Do protozoans have asexual or sexual reproduction ? In protozoans reproduction is asexual or sexual. The most frequent form of sexual reproduction is binary scissiparity or d

Animal diseases, Animal Diseases - Diseases are common in domestic anim...

Animal Diseases - Diseases are common in domestic animals. Common signs of illness in animals are - 1.      Cessation of rumination. 2.      Drop in milk yield. 3.

Define blood pressure, Blood Pressure Blood pressure may be defined as ...

Blood Pressure Blood pressure may be defined as the force or pressure which the blood exerts on the walls of the artery. Blood pressure varies with the age of the individual. B

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd