Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Define the anatomical considerations, Define the Anatomical Considerations ...

Define the Anatomical Considerations 1. Neurovascular bundle associated with greater palatine foramen, e.g. palatal root of the upper 1 st molar: Don't do incision at this area

Define the translocation of food through sieve tubes, Arrange the following...

Arrange the following processes sequentially to define the translocation of food through sieve tubes.   i. Unloading of sugar in sink cells (or cells of root).  ii. Uptak

Why the vitamin niacin is a necessary component of nad, The vitamin niacin ...

The vitamin niacin is a necessary component of NAD. Niacin can be consumed in food or manufactured in the body from tryptophan, an amino acid. How would a person's ability to break

Fisson, what is mean by fisson

what is mean by fisson

Describe rna sequence, Q. What is the name of an RNA sequence that codifies...

Q. What is the name of an RNA sequence that codifies one amino acid? Each sequence of three nitrogen-containing basis of RNA that codifies one amino acid is called a codon. The

Explain about chthalamus, Explain about chthalamus The realised niche o...

Explain about chthalamus The realised niche of Chthalamus is reduced from its fundamental niche and it is limited to the upper tidal zone Lower  limits Out-competed

Coronary prevention, a) The West of Scotland Coronary Prevention Study (WOS...

a) The West of Scotland Coronary Prevention Study (WOSCOPS): The study randomized healthy men between the ages of 45 and 64 years with TC levels higher that 252 mg/dL and LDL chole

Categories of benthic flowering plants, Categories of benthic flowering pla...

Categories of benthic flowering plants Zone of emergent plants Consisting of plants whose roots and stems remain submerged in water and whose upper leaves and ste

What is metagenesis, What is metagenesis? What are the other names of this ...

What is metagenesis? What are the other names of this process? Metagenesis is the type of life cycle in which there are two dissimilar forms of individuals of the same species,

Define the genetic growth pattern of infant, Define the genetic growth patt...

Define the genetic growth pattern of infant? It takes 1-3 years for an infant to establish his own genetic growth pattern. Once this figure is established (such as height-for-a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd