Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Explain nutritional support management for gastric cancer, Explain Nutritio...

Explain Nutritional Support Management for gastric cancer? The 'dumping syndrome' can be greatly minimized or prevented by adhering strictly to an antidumping dict. In general,

How campylobacteriosis is caused, How Campylobacteriosis is caused It i...

How Campylobacteriosis is caused It is caused by  Campylobacter jejuni, a Gram-negative rod. It is a microaerophillic organism, which means it has a requirement for reduced lev

How sequences of events occur to activate protein kinase a, When glucagon b...

When glucagon binds to it's receptor on a liver cell, which one of the following sequences of events occurs to activate protein kinase A? -activation of G protein, activation of

#title., poor metabolism phenotype will have

poor metabolism phenotype will have

What is peristomium. describe, What is peristomium. Describe? The segme...

What is peristomium. Describe? The segment behind the prostomium in annelids which contains the oral opening. This segment like the prostomium isn't a true segment and has no s

Explain in details amino acid, Explain in details Amino Acid? Amino Ac...

Explain in details Amino Acid? Amino Acids :   There are 20 common amino acids, with a few more found in lower organisms. Adult humans require a diet that includes at least 8

Explain the criteria for assessment of vitamin status, Explain the Criteria...

Explain the Criteria for Assessment of Vitamin Status? Vitamin E is assessed by determining the plasma lipid fraction levels. 0.8 mg of total tocopherol/g total plasma lipids i

Food intoxications, Food borne intoxications are basically food borne illne...

Food borne intoxications are basically food borne illness caused due to ingestion of toxin produced my microorganisms (mycotoxins, bacterial toxins). Natural toxins present in food

Ilustrate the nutritional and functional role of sodium, Minerals  :-  Sodi...

Minerals  :-  Sodium Food Source      NaCl, MSG, other food additives, milk, low in most raw foods Nutritional Functional role Essential nutrient: Deficiency is ra

Define photoorganoheterotrophs and chemoorganoheterotrophs, Photoorganohete...

Photoorganoheterotrophs and Chemoorganoheterotrophs - Nutritional Types (1) Photoorganoheterotrophs - These microorganisms use light as a source of energy and organic compound

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd