Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Explain intubation and ventilation, Explain Intubation and Ventilation ? ...

Explain Intubation and Ventilation ? If supplemental oxygen therapy or non-invasive ventilation do not help, the next step would be to intubate and commence mec

Response to wounding, Response to Wounding Wounding of tomato plants b...

Response to Wounding Wounding of tomato plants by mechanical injury or chewing by insects releases a factor that triggers accumulation of two proteinase inhibitors-throughout

Where does the water resorbed after glomerular filtration, Q. Where does mo...

Q. Where does most of the water resorbed after glomerular filtration go? What are the other substances resorbed by the nephron tubules? Only 0.5 to 1% of the glomerular filtrat

Respiratory care on admission of patient, Respiratory Care on Admission (Fi...

Respiratory Care on Admission (First two hours) Patient is incubated and ventilator-dependent.  Monitor blood gases hourly and take corrective action immediately.

Experiment turn out differently then you expected, If the results of an exp...

If the results of an experiment turn out differently then you expected, what does that mean? A. The experiment was a failure B. You did not follow scientific method C. You should e

Assignment, Write short note onApplications of apical dominance in horticul...

Write short note onApplications of apical dominance in horticulture and agriculture

Explain about osseointegration, Explain about Osseointegration Osseoint...

Explain about Osseointegration Osseointegration is regarded as the process of direct bone apposition on implant surfaces where the bone in contact with the implant later underg

Explain the term - neurochemical manipulations, Explain the term - Neuroche...

Explain the term - Neurochemical Manipulations Neurochemical and immunological methods have been used to identify groups of neurons in the central nervous system that use speci

Reproduction, What is the difference between asexual and sexual reproductio...

What is the difference between asexual and sexual reproduction

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd