Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the requirements of Special Stains?
Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.
Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.
Define about the Spectroscopy- colorimetry? Spectroscopy, therefore, is the study of the absorption and emission of radiation by matter. Absorption Spectroscopy is usually used
what does ICZN stand for
Food Applications of Locust bean gum Locust bean gum has multiple applications in food industry. Let us focus on a few of the important ones: Locust bean gum has been u
What is predatism? Predatism is the ecological interaction in which one individual mutilates or kills another to get food. Predatism is an inharmonious (negative) ecological i
THEORY OF NATURAL SELECTION OR DARWINISM Theory of CharIes Darwin (1809 - 1882) about evolution marks the beginning of an era in understanding of evolution. At the age
Highly active antiretroviral therapy (HAART) - The treatment of HIV infection requires combination therapy known as highly active antiretroviral therapy (HAART) along with inte
what does the future look like with trash in the ocean?
a project on different types of heterotrophic mode of nutrition in organisms
NE R VE IMPULSE (IT'S GENERATION) - It is an electro chemical phenomenon manifested through ionic movement across cell membrane. It was discovered by A. L. Hodykin & A
Would it be possible to establish a pure-breeding population of brown pigs with a few black spots?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd