Procedures for diagnosis - nucleic acid hybridization, Biology

Assignment Help:

Nucleic acid hybridization:

This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.


Related Discussions:- Procedures for diagnosis - nucleic acid hybridization

Cell cycle, The cell cycle undergoes a sequence of changes which invol...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

Describe phases in the care process, There are 5  distinct yet constantly i...

There are 5  distinct yet constantly interacting phases in the care process. Assessment : A broad base of relative information about the patient's nutritional status,  food ha

Aids associated and adolescent crisis emergencies, Other Psychiatric Emerge...

Other Psychiatric Emergencies:   1) Psychotropic Drug Withdrawal Abrupt cessation of antipsychotic, benzodiazepines result in symptoms of withdrawal including abdominal pain, i

Observation of bumpus, An interesting observation made by an American biolo...

An interesting observation made by an American biologist H.C. Bumpus (1899) provides a good explanation for normalising selection. Bumpus collected some 136 injured house sparrows

Explain about the glycemia or insulinemia, Explain about the Glycemia or In...

Explain about the Glycemia or Insulinemia? Preliminary research evidence indicates that there may be beneficial effects in terms of decreased fasting blood glucose/hepatic gluc

What are the cytoplasmic structures present in animal cells, Q. What are th...

Q. What are the main cytoplasmic structures present in animal cells? The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, mitochondria, peroxisomes

Describe about defecation reflex, Q. Describe about defecation reflex? ...

Q. Describe about defecation reflex? An example of a reflex having an autonomic (and somatic) component is the DEFECATION REFLEX: The rectum is generally empty, but when fec

Explain in detail about gene therapy, Explain in detail about Gene therapy ...

Explain in detail about Gene therapy Gene therapy technology as a whole has yet to be proven and it's meeting a number of stumbling blocks, so may have little impact (low succe

Structure of the pancreas, Describe the structure of the pancreas. How is t...

Describe the structure of the pancreas. How is the pancreas modified to carry out its various functions? How does the human pancreas compare to that of the fetal pig?

Zoology, type study of polystomella

type study of polystomella

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd