Procedures for diagnosis - nucleic acid hybridization, Biology

Assignment Help:

Nucleic acid hybridization:

This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.


Related Discussions:- Procedures for diagnosis - nucleic acid hybridization

Explain the chemotherapeautic rinses, Explain the Chemotherapeautic rinses ...

Explain the Chemotherapeautic rinses Chemotherapeautic rinses:  The use of  0.12  per cent chlorhexidine has been proven to be of therapeutic significance in maintaining period

Phases of menstrual cycle - reproduction, Phases of Menstrual Cycle - Repro...

Phases of Menstrual Cycle - Reproduction Four phases of the menstrual cycle are usually distinguished: the menstrual, proliferative (follicular), ovulatory and progestational

Explain the technology of transgenic plant, Explain the technology in which...

Explain the technology in which transgenic plant was constructed in which seeds that will produce plants for a single crop.

Explain about bone lining cells, Explain about Bone lining cells Bone ...

Explain about Bone lining cells Bone lining cells are basically inactive osteoblasts (in terms of making bone) that line bone surfaces. Osteocytes are osteoblasts that have be

Flow cytometry, Ask que1. Design a 4 colour Flow cytometry experiment with ...

Ask que1. Design a 4 colour Flow cytometry experiment with an aim to identify B220-, CD3+, CD4+ (DOUBLE POSITIVE) AND B220+, CD3-, CD4- (DOUBLE NEGATIVE) cells. Select from the fol

Different phases of spermatogenesis, DIFFERENT PHASES OF SPERMATOGENESIS ...

DIFFERENT PHASES OF SPERMATOGENESIS Spermatogenesis complete into two phases. 1 .       Spermatocytogenesis The entire process can be divided into three phases : (i

Etiology, Etiology: This is the study of origin of diseases. In other words...

Etiology: This is the study of origin of diseases. In other words we can say etiology is the study of causation and origination. Etiology most commonly used in philosophical theori

What are mycotoxins, Q. What are mycotoxins? How are these produced and w...

Q. What are mycotoxins? How are these produced and where are these found? Are these naturally-occurring or produced? What is their significance? First what are mycotoxins?

Enumerate the important surgical anatomical structure, Enumerate the import...

Enumerate the important surgical anatomical structures in maxilla and the structures present in the posterior wall of the maxillary sinus. The important surgical anatomic struc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd