Procedures for diagnosis - nucleic acid hybridization, Biology

Assignment Help:

Nucleic acid hybridization:

This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.


Related Discussions:- Procedures for diagnosis - nucleic acid hybridization

Define needs for nutritional relief programme & intervention, Define needs ...

Define needs for Nutritional Relief Programme and Interventions? A general feeding programme is required during first stage when the affected population does not have sufficien

What is the prevailing wind direction, what is the prevailing wind directio...

what is the prevailing wind direction in equatorial regions affected by the trade winds? a) The wind blows from east to west b) the wind blows from west to east

Evaluation of health services, CONCEPT AND MEANING OF EVALUATION: Eval...

CONCEPT AND MEANING OF EVALUATION: Evaluation of programmes is critical for measuring various community health services The ultimate reasons for doing evaluation is to ensure

Common disease of pig, COMMON DISEASE OF PIG  - 1 .       FOOT AND MO...

COMMON DISEASE OF PIG  - 1 .       FOOT AND MOUTH DISEASES 2 .       ANTHRAX 3 .       TUBERCLOSIS 4 .       SWINE FEVER - Charactrized by high fever, d

Types of muscular tissue, TYPE S -   There are present three types ...

TYPE S -   There are present three types of muscle fibres:   1.      Striped or striated or skeletal or voluntary muscles.   2.      Unstriped or non-striated o

Deficiency diseases-predisposing factors, Predisposing factors The inc...

Predisposing factors The incidence of metabolic diseases in cows and buffaloes increases as the milk production increases and the herd size becomes larger. The highest inciden

Alkaloids, Miscellaneous antinutritional substances Alkaloids: Alkal...

Miscellaneous antinutritional substances Alkaloids: Alkaloids represent a large and structurally diverse group of compounds present within angiosperm plants; the majority of

Extracellular signals, extracellular signals and their characteristics

extracellular signals and their characteristics

Nucellus - ovule, Nucellus - Ovule Enclosed within the integuments li...

Nucellus - Ovule Enclosed within the integuments lies a homogenous mass of tissue which is termed as the nucellus. This is the tissue in which the female gametophyte differen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd