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Nucleic acid hybridization:
This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.
Define Lipids - Tests for Presence of Exoenzymatic Activity? Lipids are also high molecular weight compounds. Enzyme lipases (esterases) cleaves the ester bond to form glycerol
simplified carbon cycle
Manifestations of anemia that are directly due to the diminished oxygen-carrying capacity of hemoglobin include: Answer A. pale skin. B. bleeding. C. bone pain. D. fatigue.
Subphylum Uniramia Uniramia, the largest subphylum of phylum Arthropods includes myriapods and insects. Due to the unbranched nature of the appendages of its members, the sub
When the level of AMP is high, this shows the requirement for more ATP synthesis. AMP stimulates PFK increases the rate of glycolysis and inhibits fructose 1 and 6- bisphosphatase
Cilia and flagella share a common structure, with a microtubular core which has a 9+2 organization with nine paired doublets of microtubules surrounding a central pair to form the
Q. Is herbivorism a form of predatism? The Herbivorism is a form of predatism in which first order consumers feed from producers (plants or algae). For instance, fruits and bir
Q What are the few examples of annelids? Earthworms, lugworms and leeches. Q. Which are the morphological features that differentiate the beings of the phylum Annelida from
What is reproductin
Adult Botulism The symptoms of botulism may develop between 12 and 72 hours after the ingestion of toxin containing foods. Symptoms include nausea, vomiting, fatigue, dizzine
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