Procedures for diagnosis - nucleic acid hybridization, Biology

Assignment Help:

Nucleic acid hybridization:

This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.


Related Discussions:- Procedures for diagnosis - nucleic acid hybridization

Introduction to avian infectious diseases, Poultry birds are affected with ...

Poultry birds are affected with many types of ailments; broadly these are classified on the basis of etiology as non-infectious and infectious conditions. Non-infectious diseases a

Spermatogenesis, Spermatogenesis: In the mature male functional sper...

Spermatogenesis: In the mature male functional sperm cells are produced within the seminiferous tubules of the testes. Around the periphery of the seminiferous tubules are l

Name the ceramics used in bioactive, Bioactive Ceramics:-   Hydroxyapa...

Bioactive Ceramics:-   Hydroxyapatite, Tricalcium phosphate, Tetracalcium phosphate, calcium pyrophosphate. Fluorapatite, brushite,  bioglass.

How age affects the bmr, How Age affects the BMR? The BMR per unit weig...

How Age affects the BMR? The BMR per unit weight also varies with age, being higher in children and lower in the elderly. The loss of FFM with ageing is associated with a decli

Reaction of the futile cycle, Reaction of the futile cycle:- A) An  ade...

Reaction of the futile cycle:- A) An  adequate level of  cAMP  stimulates formation of  the  inactive  'D'  form  of glycogen synthase and the active form of phosphorylase. Thu

Explain the food security, Explain the Food Security? You were introduc...

Explain the Food Security? You were introduced to the concept of food security in Unit 2. Food security, we learnt, is access by all people at all times to enough food for an a

Differences between ecosystems, Some ecosystems possess relatively large nu...

Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,

Exceptions to the cell theory, Exceptions to the Cell Theory Viruses, ...

Exceptions to the Cell Theory Viruses, however, are not the only exception to the Cell Theory as formulated earlier. This has led scientists to take a much broader view of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd