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Explain the Generalities?
A binary system has two components; C equals 2, and the number of degrees of freedom is F = 4 - P. There must be at least one phase, so the maximum possible value of F is 3. Since F cannot be negative, the equilibrium system can have no more than four phases. We can independently vary the temperature, pressure, and composition of the system as a whole. Instead of using these variables as the coordinates of a three-dimensional phase diagram, we usually draw a two-dimensional phase diagram that is either a temperature- composition diagram at a fixed pressure or a pressure-composition diagram at a fixed temperature. The position of the system point on one of these diagrams then corresponds to a definite temperature, pressure, and overall composition. The composition variable usually varies along the horizontal axis and can be the mole fraction, mass fraction, or mass percent of one of the components, as will presently be illustrated by various examples.
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An incubation combination is set up having the single-stranded DNA template, DNA polymerase I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP),
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