Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Decolourizing Agent - Stain Technique?
95% ethanol is used as a decolourizing agent. It has two functions -
(1) It acts as protein - dehydrating agent, and
(2) It is a lipid solvent. The action of decolourizing agent depends on two factors -
(i) Concentration of lipids in cell wall.
(ii) Thickness of peptidoglycan layer.
In gram negatives, lipid content is high as compared to gram positives, so ethanol action increases the porosity of cell wall by dissolving the lipid layer in outer membrane. Peptidoglycan layer being thinner and less highly cross linked as compared to gram positives, is not able to retain the CV-I complex and cell become colourless. On the other hand, in gram positives, the pores become smaller due to dehydrating effect of the alcohol, so CV-I complex is retained by the thick highly cross linked peptidoglycan layer, and the cells remain purple. This step generates the differential aspect of the gram stain and is very critical.
Types of Glials Cells and their Functions In the nervous tissue, besides the nerve cells there is one other cell type -the neuroglia which is present in large numbers and occu
What is the MN blood system? What is the pattern of genetic inheritance of the MN blood system? A MN blood system is a third (in addition to the ABO and the Rh) system of blood
Caffeine and alcohol both block the secretion of ADH from the posterior pituitary gland. Explain what this does to fluid balance. Does it make sense to drink caffeine before an ath
Animals in the Community - Nature and Structure of Community The animals in the community are directly or indirectly dependent on the plants also time their activities in such
Define ascending type of paper chromatography? The ascending type consists in dipping the lower end of the paper containing the sample spots into the solvent so that it is abov
Reproductive Cycle The whole reproductive cycle consists of an ovarian cycle and a uterine cycle. Figure illustrates one complete reproductive cycle. You can observe in the fi
Why are the recombinant DNA technology and the nucleus transplantation technology still dangerous? A recombinant DNA technology and the nucleus transplantation technology (clon
Behavioural Adjustments When faced with a sudden temperature change, most animals make behavioural responses that enable them to avoid extreme or lethal conditions. Among the
Prepare a dichotomous key the following organisms, ant, butterfly, fly, beetle, grass hopper, wasp, ladybug, roach and dragonfly
Define Regents for Determination of Inorganic Phosphorus? 1. Trichloroacetic acid (10%) - Dissolve 100 g of high grade crystalline acid in distilled water and dilute to 1 Litre
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd