Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Decolourizing Agent - Stain Technique?
95% ethanol is used as a decolourizing agent. It has two functions -
(1) It acts as protein - dehydrating agent, and
(2) It is a lipid solvent. The action of decolourizing agent depends on two factors -
(i) Concentration of lipids in cell wall.
(ii) Thickness of peptidoglycan layer.
In gram negatives, lipid content is high as compared to gram positives, so ethanol action increases the porosity of cell wall by dissolving the lipid layer in outer membrane. Peptidoglycan layer being thinner and less highly cross linked as compared to gram positives, is not able to retain the CV-I complex and cell become colourless. On the other hand, in gram positives, the pores become smaller due to dehydrating effect of the alcohol, so CV-I complex is retained by the thick highly cross linked peptidoglycan layer, and the cells remain purple. This step generates the differential aspect of the gram stain and is very critical.
Why can the crossing of an individual that manifests dominant phenotype with another that manifests recessive phenotype (for the same trait) determine whether the dominant individu
Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial
Explain about the Shade Drying? Shade drying is carried out for products which can lose their colour and/or turn brown if put in direct sunlight. Products that have naturally v
Nasopalatine nerve and vessels Found in the nasopalatine canal, must be taken care off when placing implants in the maxillary incisor region. Subperiosteal implant placement o
Stages of Wilm's tumour We shall now discuss about the various stages of Wilm's tumour. The extent of disease is staged according to the findings of surgery and the presen
phase of alpha taxonomy
Difference between Vegetative and Generative Cell The cytoplasm of the vegetative cell and the generative cell is distinctly different. The generative cell is transparent and
Endocrine Glands
how many phylum does kingdom protista has?
Why could a disease be more serious in a population with a low genetic diversity? A population with a low genetic diversity could be more expected to be wiped out by a disease
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd