Explain the confirmed test - most probable number test, Biology

Assignment Help:

Explain the Confirmed Test - Most Probable Number Test?

Confirmed test is carried out to rule out the possibility of any positive presumptive test because of the presence of noncoliform organisms which are not recognized as indicators of faecal pollution. The test is performed by streaking on selective and differential media, i.e., EMB agar and Endo agar from positive lactose broth tube in presumptive test.

EMB contains lactose and dyes eosin and methylene blue. Methylene blue inhibits the growth of Gram positive bacteria. Lactose fermenters produce acid which results in the precipitation of dyes on to coliform colonies producing dark coloured center and a green metallic sheen. These results are characteristics of E.coli, the major indicator of faecal pollution. Enterobacter aerogenes produce pink and mucoidal colonies while nonlactose fermenters produce colourless, transparent colonies. Endo agar is a nutrient medium containing dye fuchsin in decolourized state which produce a pink complex in presence of acid produced by coliforms resulting in pink colonies of E. coli.


Related Discussions:- Explain the confirmed test - most probable number test

Concomitant caroid endarterectomy and cabg, Concomitant caroid endarterecto...

Concomitant caroid endarterectomy and CABG :   Symptoimatic or asymptomatic carotid artery disease may be present in patients undergoing CABG. It is very important to recognize thi

Explain disadvantage of colonies at different dilution, Explain the Disadva...

Explain the Disadvantages of Colonies Obtained At Different Dilution 1. Heat sensitive microorganisms may be damaged by melted agar, giving low viable count as compared to spre

Important aspect of dietary management during hypertension, Q. Important as...

Q. Important aspect of dietary management during hypertension? Most important aspect of dietary management i.e. the intake of minerals and electrolytes, which are closely assoc

Protoplast, PROTOPLAST Cell without cell membrane (Henstein) It c...

PROTOPLAST Cell without cell membrane (Henstein) It contains 1 or more vacuoles. In mature plant cells protoplast forms a peripheral layer called Primordial utricle.

Effect on microbial growth of pH, Q. Effect on Microbial Growth of pH? ...

Q. Effect on Microbial Growth of pH? Every microorganism has a minimal, a maximal, and an optimal pH for growth. In general, bacteria grow in the pH range of 6.0-8.0, yeasts 4

Distinguish between epithelial and connective tissues, Distinguish between ...

Distinguish between epithelial and connective tissues with respect to their cell arrangement? PROVIDE a specific example (for both tissue types) of how the arrangement of cells hel

Define mineral requirements during pregnancy period - iron, Define Mineral ...

Define Mineral Requirements during pregnancy period - Iron? While B 12 and folk acid are needed for the normal erythropoiesis, they must be accompanied by adequate amounts of

Differentiation of tissues - root apex, Differentiation of Tissues - Root A...

Differentiation of Tissues - Root Apex New cells generated from the divisions of meristematic cells start expanding and differentiating further. Epidermis, cortex and stele a

Explain types of dietary adaptations for trerapieutic needs, Types of dieta...

Types of dietary adaptations for trerapieutic needs Normal nutrition is the foundation upon which therapeutic modifications are based. We  have already discussed  in  previous

Explain about osseointegration, Explain about Osseointegration Osseoint...

Explain about Osseointegration Osseointegration is regarded as the process of direct bone apposition on implant surfaces where the bone in contact with the implant later underg

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd