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Explain the Confirmed Test - Most Probable Number Test?
Confirmed test is carried out to rule out the possibility of any positive presumptive test because of the presence of noncoliform organisms which are not recognized as indicators of faecal pollution. The test is performed by streaking on selective and differential media, i.e., EMB agar and Endo agar from positive lactose broth tube in presumptive test.
EMB contains lactose and dyes eosin and methylene blue. Methylene blue inhibits the growth of Gram positive bacteria. Lactose fermenters produce acid which results in the precipitation of dyes on to coliform colonies producing dark coloured center and a green metallic sheen. These results are characteristics of E.coli, the major indicator of faecal pollution. Enterobacter aerogenes produce pink and mucoidal colonies while nonlactose fermenters produce colourless, transparent colonies. Endo agar is a nutrient medium containing dye fuchsin in decolourized state which produce a pink complex in presence of acid produced by coliforms resulting in pink colonies of E. coli.
State the term Autopsy data - Brain patient Autopsy data are not always entirely usable for validation purposes, in that numerous changes may have taken place in the patient's
A classical secretory protein vary from a cytosolic protein by having a sequence about 13-35 amino acids long at its N-terminal end called as a signal peptide or signal sequence.
Q. What is green fluorescent protein (GFP)? Green fluorescent protein is a protein which is found in a luminescent jellyfish (Aquorea victoria) that lives in cold waters of nor
State about the Latent squint disease Latent squint is seen commonly as exophoria in infancy and presbyopic age and esophoria in childhood. Hypermetropes can have esophoria and
Genic Type-Genetic basis of Sex Determination In some organisms, certain indepe~ident genes located on different chromosomes are responsible for sex determination. Most species
general charactres and classfication
What is a population? A population is a set of individuals of the similar species found in a given place in a given time.
Wet Stigma - Category of Stigma Petunia shows several randomly distributed 2-celled papillae on its surface. In a developing stigma, the epidermis is covered by a continuous,
Captive Breeding - Measures for Species Conservation Species which are reduced to dangerous levels need more intensive management, and one strategy is their captive breeding.
Pneumonia Pneumonia is an inflammatory process in which there is consolidation in lung caused by exudates filing the alveolar spaces. Gas exchange cannot take place in the c
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