Explain the confirmed test - most probable number test, Biology

Assignment Help:

Explain the Confirmed Test - Most Probable Number Test?

Confirmed test is carried out to rule out the possibility of any positive presumptive test because of the presence of noncoliform organisms which are not recognized as indicators of faecal pollution. The test is performed by streaking on selective and differential media, i.e., EMB agar and Endo agar from positive lactose broth tube in presumptive test.

EMB contains lactose and dyes eosin and methylene blue. Methylene blue inhibits the growth of Gram positive bacteria. Lactose fermenters produce acid which results in the precipitation of dyes on to coliform colonies producing dark coloured center and a green metallic sheen. These results are characteristics of E.coli, the major indicator of faecal pollution. Enterobacter aerogenes produce pink and mucoidal colonies while nonlactose fermenters produce colourless, transparent colonies. Endo agar is a nutrient medium containing dye fuchsin in decolourized state which produce a pink complex in presence of acid produced by coliforms resulting in pink colonies of E. coli.


Related Discussions:- Explain the confirmed test - most probable number test

Demand for health care in the presence of health insurance, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Regents for estimation of vitamin c - colorimetric method, Define Regents f...

Define Regents for Estimation of Vitamin C - Colorimetric Method? 1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric aci

Myocardial energetics, The oxidation of substrates like free fatty acids (F...

The oxidation of substrates like free fatty acids (FFA), glucose, lactate and ketone bodies results in the energy of Adenosine triphosphate (ATP) and Creatine phosphate (CP). The s

Bacterial diseases-botulism, Botulism Botulism is a rapidly fatal motor...

Botulism Botulism is a rapidly fatal motor paralysis. It is caused by ingestion of toxin of Clostridium botulinum in food. Many species of domesticated, wild animals and birds

Free-hand sectioning of plant tissue (stem), What are the processes involve...

What are the processes involved in the preparation of plant tissue for free hand sectioning?

Determine effective half life and value of effective decay, 1. By direct me...

1. By direct measurement of the activity of the thyroid gland in several patients who had received 131 I for diagnostic purposes, it was observed that the biological elimination h

Fidelity - synthetic characters, Fidelity - Synthetic Characters Fidel...

Fidelity - Synthetic Characters Fidelity refers to the degree to which a species is restricted in its occurrence to a particular kind of community. The species with low fideli

Define the risk reduction in cardiovascular disease, Q. Define the Risk red...

Q. Define the Risk reduction in cardiovascular disease? Ans. Weight loss by caloric restriction diets has been shown to decrease plasma CRP levels. Additional data indicat

Seliwanoff test or resorcinol hydrochloric acid reaction, Define seliwano...

Define seliwanoff's Test or Resorcinol Hydrochloric Acid Reaction? seliwanoff's test is specific test for ketonic groups and therefore is positive for ketose sugars like fruc

What are the disadvantages of mucoperiosteal flap, What are the Disadvantag...

What are the Disadvantages of mucoperiosteal flap More traumatic - flap is raised  Sutures are required and the next procedure of impression is delayed till afterthe suture

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd