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Explain the Confirmed Test - Most Probable Number Test?
Confirmed test is carried out to rule out the possibility of any positive presumptive test because of the presence of noncoliform organisms which are not recognized as indicators of faecal pollution. The test is performed by streaking on selective and differential media, i.e., EMB agar and Endo agar from positive lactose broth tube in presumptive test.
EMB contains lactose and dyes eosin and methylene blue. Methylene blue inhibits the growth of Gram positive bacteria. Lactose fermenters produce acid which results in the precipitation of dyes on to coliform colonies producing dark coloured center and a green metallic sheen. These results are characteristics of E.coli, the major indicator of faecal pollution. Enterobacter aerogenes produce pink and mucoidal colonies while nonlactose fermenters produce colourless, transparent colonies. Endo agar is a nutrient medium containing dye fuchsin in decolourized state which produce a pink complex in presence of acid produced by coliforms resulting in pink colonies of E. coli.
Determine the genotypes and phenotypes of the F1 generation from a colour blind father and a mother who is homozygous for normal colour vision.
Tolerance Model - Models of Succession In this model, the presence of early successional species is not essential, that is, any species can start succession. Some species are
What are the three phases into which the HIV infection is often divided? The HIV infection is often separated into three phases: the acute phase, from the infection to 1 until
Explain the Superficial Minor Burns? Superficial minor bums are immersed immediately in cool water if possible. The burn is carefully cleaned to prevent infection. If dirt is d
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Assuming that the equilibrium potential for sodium ion across a skeletal muscle is +66 mv at a temperature of 37 degree celcius when the external concentration is 145 mM and the in
Permanent Wilting Percentage (PWP) It is the percentage of moisture in the soil at which a plant wilts and does not recover unless water is added to the soil. PWP for clay is
Oil seed meals as source of limiting amino acids Generally ruminants are not perceived to have a requirement for essential amino acids as the rumen microbes can synthesis esse
Q. What is the basic importance of water for enzymatic activity? Ans. Biological catalysts, Enzymes, relay on water to reach their substrates and attach to them. There is
Define classification of carbohydrates - Disaccharides? Disaccharides are condensation products of two monosaccharide units joined together by a linkage called glycosidic linka
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