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Concept behind Freezing the milk
Freezing the milk also alters its composition and properties to a great extent. Let us learn about these changes. As the milk freezes, it becomes very uneven in the composition of the frozen and other solids, while the liquid portion becomes concentrated with the milk solids, so much so that milk never freezes solid entirely. Freezing alters the physical condition of milk to the extent that it never returns to its original state. It causes the fat globules to lose their complete emulsion structure, to clump and become distorted and irregular in shape and size. The casein is also affected by freezing. It is partly broken from its existence in milk as calcium caseinate and gets precipitated as flakes. This condition, together with some free fat particles, gives the thawed-out milk an unnatural appearance. The flavor is also affected, being rather watery to the taste.
ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.
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