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Explain the Composition of Blood Agar?
Infusion from beef heart - 500 gm
Tryptose - 10.0 gm
Sodium Chloride - 5.0 gm
Agar - 15 gm
Distilled water - 1000 ml
pH - 7.3
Dissolve above ingredients and autoclave. Cool to 45-50°C and add 50 ml of sterile defibrinated blood aseptically. Rotate to mix thoroughly and pour immediately into sterile plates or tubes before solidification.
A solution was prepared by dissolving 25g NaOH powder in 100 ml water. Using not more than 15 ml water, devise a series of dilutions that will give a concentration of 5mg/ml NaOH.
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