Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain Spread Plate Method - Pure Culture Techniques?
In this method, diluted microbial suspension containing about 30 to 300 colonies is spread uniformly on the agar surface with a sterile bent rod (spreader). Look at the Figure, which graphically illustrates the spread plate method. By repeated picking and restreaking of a well-isolated colony, a pure culture can be obtained.
The dispersed cells develop into isolated colonies. The method can also be used for quantitation of microbial number in the sample, as you will learn in the next practical.Briefly, in spread plate method, a small volume of the diluted sample (about 0.1 ml) is transferred to the centre of a pre-poured solidified agar plate and then spread uniformly over the surface of the medium with a sterile L-shaped glass rod or spreader. After incubation, the dispensed cells form isolated colonies on agar surface, the number of which is used to calculate the amount of microbes in a given sample. It is important that the surface of the plate be dry so that the culture that is spread soaks in.
Define the Spatial processes? Environmental and integrative biology cuts across a wide array of spatial scales, and theoretical approaches that take into account these widely v
Concerning the sex chromosomes of the XY system which type of gamete do the male and the female individuals respectively produce? An Individual of the female sex is XX and thus
Describe giving one example of each, the three types of joints in human skeleton, based on the capacity of movement. A patient was complaining of frequent urination, excessive
What is HMP pathway? Give any two points of its significance The HMP is an alternate oxidative pathway for the metabolism of glucose. The significance of the pathway is that
All the cells present in the body contain genes and an approach targeted to modulate the expression of genes is known as gene therapy. This novel approach has emerged by the end of
A. How is the villus the functional unit of the small intestine? In your answer, include a discussion of its structure and function and the enzymes found there. What is the importa
During chemical synaptic transmission A. neurotransmitter travels via diffusion in extracellular space. B. there will always be release of neurotransmitter from the postsyna
Q. Management of Symptomatic Patients All symptomatic patients with severe aortic stenosis or even moderately severe aortic stenosis should undergo aortic valve replacement an
In which chloroplast structure are chlorophyll molecules found? Chlorophyll molecules are placed in an organized manner in order to increase the exposure to light on the thylak
Patient after cardiac transplant The major focus of medical and nursing care after transplantation is to prevent early identification of rejection so that appropriate
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd