Explain spread plate method - pure culture techniques?, Biology

Assignment Help:

Explain Spread Plate Method - Pure Culture Techniques?

In this method, diluted microbial suspension containing about 30 to 300 colonies is spread uniformly on the agar surface with a sterile bent rod (spreader). Look at the Figure, which graphically illustrates the spread plate method. By repeated picking and restreaking of a well-isolated colony, a pure culture can be obtained.

 

1782_Spread Plate Method.png

The dispersed cells develop into isolated colonies. The method can also be used for quantitation of microbial number in the sample, as you will learn in the next practical.Briefly, in spread plate method, a small volume of the diluted sample (about 0.1 ml) is transferred to the centre of a pre-poured solidified agar plate and then spread uniformly over the surface of the medium with a sterile L-shaped glass rod or spreader. After incubation, the dispensed cells form isolated colonies on agar surface, the number of which is used to calculate the amount of microbes in a given sample. It is important that the surface of the plate be dry so that the culture that is spread soaks in.


Related Discussions:- Explain spread plate method - pure culture techniques?

What property of water helps, South Dakota and Oregon are at similar latitu...

South Dakota and Oregon are at similar latitudes, and yet, on average, Oregon has warmer winters and cooler summers than South Dakota does. What property of water helps account for

Explain the differance between pinocytosis and phagocytosis, Explain the di...

Explain the differance between Pinocytosis and Phagocytosis? Pinocytosis and Phagocytosis : The cell membrane occasionally invaginates or indents to capture extracellular flu

Gene targeting in somatic cells, Gene targeting in somatic cells: Since t...

Gene targeting in somatic cells: Since the pioneering work with sheep at Roslin cloning by nuclear transfer has now been exemplified in a number of other livestock species includ

What is the importance of water for enzymatic activity, Q. What is the basi...

Q. What is the basic importance of water for enzymatic activity? Ans. Biological catalysts, Enzymes, relay on water to reach their substrates and attach to them. There is

Levels of prevention and role of a nurse, LEVELS OF PREVENTION AND ROLE OF ...

LEVELS OF PREVENTION AND ROLE OF A NURSE: Caplan describes the levels of  prevention as follows:  1)   Primary Prevention  It is concerned with  reduction of  incidence

Ciliary and flagellar movements, Normal 0 false false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Contractile vacuoles - excretion, Normal 0 false false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Nursing management of myocarditis, Nursing Management Propped up po...

Nursing Management Propped up position.  Monitor signs and symptoms and laboratory test results documenting myocarditis.  Monitor adequacy of cardiac output and hemo

Bacterial diseases-actinomyces, Actinomyces Members of the genus Actin...

Actinomyces Members of the genus Actinomyces are facultative anerobic, gram-positive, non acid-fast, non-spore forming, nonhaemolytic rods. All Actinomyces require rich media

What is the relationship between hypothesis and a theory, What is the relat...

What is the relationship between the following scientific terms: hypothesis, a theory, and a natural law?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd