Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Organs involved in the digestion process, The various organs involved  in...

The various organs involved  in  the digestion process are: Alimentary tract which  includes mouth, pharynx, esophagus stomach, small intestine, large intestine, appendix,  rec

Explain Objectives of root-end resection apicoectomy, Explain Objectives of...

Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure,  c) To

Explain exotic species, Explain exotic species? Describe with the help ...

Explain exotic species? Describe with the help of two examples how the exotic species disturb the native species of an ecosystem ?

glycolysis, explain glycolysis briefly? elaborat

explain glycolysis briefly? elaborate

What are goitrogens, What are Goitrogens? Certain chemical substances l...

What are Goitrogens? Certain chemical substances like thiocyanates, phenols, disuphides, flavanoids etc, found in the environment, can interfere with iodine metabolism. These s

What is guided bone regeneration, What is Guided bone regeneration Gui...

What is Guided bone regeneration Guided bone regeneration (GBR) allow the practitioner to place implants in patients who would not have received this treatment option a few y

Diversity in living organisms.., labelled diagram of male and female cockro...

labelled diagram of male and female cockroach with difference between them

Grouping of stem cells-totipotent scs, Grouping of Stem Cells: The rang...

Grouping of Stem Cells: The range of differentiation “Potency” is an important property of Stem cells & serves as the basis for classifying cells as : Totipotent SCs -capa

Kingdom protista, what is the taxonomy of phylum sporoza?

what is the taxonomy of phylum sporoza?

Respective functions of phospholipids, What are the respective functions of...

What are the respective functions of phospholipids, proteins and carbohydrates of the cell membrane? Ans) Membrane phospholipids have structural function they produce the bilipi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd