Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Define sample titration for saponification number of fats, Define Sample Ti...

Define Sample Titration for Saponification Number of Fats? Measure 2 ml of oil with the help of a pipette and transfer this oil to a 250 ml conical flask. Add 40 ml of alcoholi

Transgenic animals, T ra n sge n i c animals There are various d...

T ra n sge n i c animals There are various definitions for the term transgenic animal . The transgenic animal is an animal in which there has been a deliberate modifica

Explain about the parkinson''s disease, Explain about the Parkinson's disea...

Explain about the Parkinson's disease? Parkinson's disease is a degenerative central nervous system (CNS) condition characterized by progressive loss of cells within substantia

Carbon dioxide for photosynthesis, Aim: To prove that Carbon dioxide is ess...

Aim: To prove that Carbon dioxide is essential for photosynthesis. Apparatus: A potted healthy plant with long and narrow leaves, a wide mouthed glass bottle, split cork. Chemicals

Determine condensation foam method, Determine condensation foam method ...

Determine condensation foam method In the condensation method a pressurized solution is suddenly released  to expand the number of gas bubbles which their ripe through the liqu

Contractile vacuole - protozoans, Contractile vacuole - Protozoans The...

Contractile vacuole - Protozoans The contractile vacuoles may differ in complexity in various groups of protozoans. In amoebae the vacuoles are carried around in the cytoplasm

What is the physiological explanation for the daltonism, What is the type o...

What is the type of genetic inheritance of daltonism? Is daltonism more frequent in men or in women? What is the physiological explanation for the daltonism ? The Daltonism

What is severity of the oesophagitis, Q. What is severity of the oesophagit...

Q. What is severity of the oesophagitis? The severity of the oesophagitis resulting from oesophageal reflux is determined by the content of gastric reflux mucosal resistance,

Use pcr to sequence the particular gene, You wish to use PCR to sequence a ...

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd