Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Explain about the sugaring - methods of food processing, Explain about the ...

Explain about the Sugaring - methods of food processing? Water is withdrawn from the microbial cells when they are placed in a strong sugar solution (about 68%) and thus result

Explain the pre pregnancy height and foetal outcome, Explain the Pre Pregna...

Explain the Pre Pregnancy Height and Foetal Outcome? Maternal height prior to conception, determined by heredity, socio-economic environment and maternal nutritional status, is

Determine the occurrence of vitamin A, Determine the Occurrence of vitamin ...

Determine the Occurrence of vitamin A In the vegetable kingdom, vitamin A  probably occurs in the form of its provitamins which belong to the group of carotenes. Carrots, spina

Mention chemical agents available for sterilization, Q. Mention chemical ag...

Q. Mention chemical agents available for sterilization? Various chemical agents available for sterilization are: Alcohols: ethyl alcohol, isopropyl alcohol, trichlorobuta

Acute mitral regurgitation-mitral regurgitation, Acute Mitral Regurgitation...

Acute Mitral Regurgitation :  This may be caused by chordal rupture, infective endocarditis or acute myocardial infarction. In acute myocardial infarction, one of the papilla

Name, what''s you''re name

what''s you''re name

Conservation of animal genetic resources, The term farm animal genetic reso...

The term farm animal genetic resources (AnGR) is used to include all animal species, breeds and strains (and their wild relatives) that are of economic, scientific and cultural int

Relationship between mind and brain, Q. Relationship between Mind and Brain...

Q. Relationship between Mind and Brain? In modern times, before the 20th century, the most popular interpretation of the mind-brain relationship was some version of dualism. It

Carrier protein in a plasma membrane, What is a characteristic feature of a...

What is a characteristic feature of a carrier protein in a plasma membrane? Carrier proteins are globular proteins which are particular it their action and therefore regulate t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd