Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Define the carbohydrate and fat required for elderly, Define the Carbohydra...

Define the Carbohydrate and Fat required for elderly? Carbohydrate: It is usually recommended that approximately 55en% (percent of  energy) be provided from carbohydrate food

Explain the function of myosin and albumin, What are respectively some rema...

What are respectively some remarkable functions of myosin, CD4, albumin, keratin, immunoglobulin, reverse transcriptase, hemoglobin and insulin? Myosin is a protein that when a

Causes of malnutrition in inflammatory bowel disease, Q. Causes of Malnutri...

Q. Causes of Malnutrition in Inflammatory Bowel Disease? The causes of malnutrition include: • Decreased oral intake, which can be disease induced due to abdominal pain, dia

Explain leptospirosis, Leptospirosis It is a bacterial disease that oc...

Leptospirosis It is a bacterial disease that occurs in many domestic and wild animals, is endemic worldwide, but the highest incidence is in tropical and subtropical areas. Tr

Succession - community change, Succession - Community Change The facto...

Succession - Community Change The factors like fire, floods and human interventions affect an ecosystem considerably. They often lead to the depletion or stripping off of orig

Explain about types of insulin, Q. Explain about types of Insulin? Thre...

Q. Explain about types of Insulin? Three types of Insulin is available. The type varies in how quickly it starts working (lowering blood glucose), time of peak activity (when t

Explain about the acesulfame k - artificial sweeteners, Explain about the A...

Explain about the Acesulfame K - Artificial Sweeteners? Acesulfame-K (potassium salt of 6-methyl-1, 2, 3-oxathazin-4(3H)-one 2, 2 dioxide) is a sweetener which is commonly used

What is the part of the female reproductive system, What is the part of the...

What is the part of the female reproductive system where fecundation occurs? Fecundation generally happens in the Fallopian tubes but it can also take place within the uterus.

Explain properties of colloidal systems - electric charge, Electric Charge ...

Electric Charge - Properties of Colloidal Systems Colloidal particles are electrically charged. Some colloidal particles carry a positive charge (+), others a negative charge (

What are cnidocytes, What are cnidocytes? What is the name of the capsule i...

What are cnidocytes? What is the name of the capsule inside the cnidocyte? What are the biological functions of this structure? Cnidocytes are specialized cells present in coel

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd