Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Cytoplasmic bridge formed during the conjugation of paramoec, Cytoplasmic b...

Cytoplasmic bridge formed during the conjugation of paramoec: The two paramoecia that undergo conjugation are called conjugants. During conjugation, the two conjugants com

Define about the ascorbic acid (vitamin c), Define about the Ascorbic acid ...

Define about the Ascorbic acid (vitamin C)? Ascorbic acid was discovered as the anti-scurvy vitamin. Vitamin C (chemical names ascorbic acid and ascorbate) is the six-carbon la

Explain spermatocyte I and spermatocyte II, What is the difference between...

What is the difference between spermatocyte I and spermatocyte II? The spermatocyte I (2n) undergoes the primary division of meiosis (meiosis I) originating two spermatocyte II

Excretion, State the excretory organelles of various organisms

State the excretory organelles of various organisms

What is a nutrient, What is a nutrient? A nutrient is it substance used...

What is a nutrient? A nutrient is it substance used in the metabolism and which is acquired from the diet. Such as, vitamins and necessary amino acids are nutrients.

Estrous cycle - reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Temperate rain forests - ecosystem, Temperate rain forests - Ecosystem ...

Temperate rain forests - Ecosystem The temperate rain Forests are colder than any other rain forest and exhibit a marked seasonality with regard to temperature and rainfall. R

What are the major organic molecules for living beings, Q. What are the maj...

Q. What are the major significant organic molecules for living beings? There are many types of organic molecules that are important for the living beings. Particularly importan

Aujeszky''s disease, Aujeszky's disease This is primarily a disease of...

Aujeszky's disease This is primarily a disease of pigs, which serve as the principal reservoir host. The secondary hosts include cattle, horse, sheep, goat, dog and cat but hu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd