Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Why is it true that of the 28 chromosomes in an ovum, A species has a diplo...

A species has a diploid chromosome number of 56, WHY is it true that of the 28 chromosomes in an ovum, 14 are always paternal?

Animal tissue, Animal Tissue A tissue is a group of cells that are si...

Animal Tissue A tissue is a group of cells that are similar in structure, origin and function. Tissue word coined by Bichat (Father of Histology). Histology term

Define intracellular cytoplasmic calcium concentration, How intracellular c...

How intracellular cytoplasmic calcium concentration is maintained? Intracellular cytoplasmic calcium concentration is maintained by the following mechanisms: a) Limited entr

Can you explain deoxynivalenol mycotoxicosis, Q. Can you explain Deoxynival...

Q. Can you explain Deoxynivalenol Mycotoxicosis? During 1987, an outbreak estimated to have affected over 50,000 people in the State of Jammu and Kashmir, was traced to the c

Abo blood compatibility for the safety of blood transfusion, Is ABO blood c...

Is ABO blood compatibility enough for the safety of blood transfusion? Moreover ABO blood compatibility the compatibility concerning the Rh blood system must also be checked. A

Spinal nerves, Spinal nerves: They take their origin from the spinal...

Spinal nerves: They take their origin from the spinal cord. They are the mixed nerves having both sensory and motor fibres. There are 31 pairs of spinal nerves. The se

Calculate the frequencies of heterozygotes, If dimples may be considered a ...

If dimples may be considered a single-gene dominant trait, and 64% of the population features dimples, calculate the frequencies of heterozygotes and both types of homozygotes, ass

Solid waste management, Solid waste management, Other Management The solid...

Solid waste management, Other Management The solid wastes from a summer camp with 100 children and a staff of 25 are to be collected once per week. If bottles and cans (representi

What are the hexoses, Q. What are the hexoses? What are some examples of he...

Q. What are the hexoses? What are some examples of hexoses with vital biological functions? Hexoses are carbohydrates whole of six carbons. Galactose, fructose and Glucose are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd