Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Apical window, The transducer is placed in the mid axillary line with ...

The transducer is placed in the mid axillary line with the transducer ridge pointing towards the patients left to obtain the four-chamber view. The left ventricular apex is

Explain phylum-bacteria, Phylum-Bacteria (Eubacteria) Eubacteria,th e t...

Phylum-Bacteria (Eubacteria) Eubacteria,th e true bacteria, are extremely small and they exist in vast numbers virtually everywhere on earth. They range from the upper atmosphe

Chemical element is the central in the chlorophyll molecule, Q. Which chemi...

Q. Which chemical element is the central in the chlorophyll molecule? The chemical element that is the central in the chlorophyll molecule is magnesium. One atom of magnesium

Productivity in medical transcription, Describe several factors which affec...

Describe several factors which affect Productivity in Medical Transcription. Definition of Medical Transcription Show the various factors thataffect Productivity in Medi

Determine the lateral cephalometric skull view, Determine the Lateral Cepha...

Determine the Lateral Cephalometric Skull View The use of this modality is limited in preoperative evaluation in implant placement for advance cases where there is a need for b

Carbon dioxide concentration in photosynthesis process, Q. Why is carbon di...

Q. Why is carbon dioxide concentration a limiting factor of the photosynthesis process? And When the carbon dioxide concentration is increased indefinitely is photosynthesis also i

What are the factors to an actual shift in the demand curve, What are the f...

What are the factors to an actual shift in the demand curve? Factors that cause an actual shift within the demand curve are as follows: When prices rise or fall that would c

Explain lamivudine, Explain Lamivudine It is probably the best tolerat...

Explain Lamivudine It is probably the best tolerated of the NRTIs and can be taken once or twice daily. An increase in viral load during treatment with a lamivudine-containing

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd