Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Explain the relation between chromatin and chromosome, What is the relation...

What is the relation between the concepts of chromatin and chromosome? Are euchromatin and heterochromatin part of chromosomes? Every filament of chromatin is a complete DNA mo

Optical rotation in organice compounds, how the optical rotaion occurs in g...

how the optical rotaion occurs in glucose and ribose?

Temperature and concentration of nutrients, Temperature and Concentration o...

Temperature and Concentration of Nutrients Temperature Temperature like salinity remains almost constant in the oceans in contrast to the land or terrestrial ecosystems

Heat loss, Heat Loss Temperature regulation is extremely uneconomical...

Heat Loss Temperature regulation is extremely uneconomical if it depends only on variations in metabolism. Therefore, mechanisms for losing excess heat have been developed by

Define the use of computers in numerical taxonomy, Define the Use of Comput...

Define the Use of Computers in Numerical Taxonomy The use of computers in numerical taxonomy has made it possible to compare a large number of characters from many organisms wi

Explain management of ledge - non-surgical endodontic, Explain Management o...

Explain Management of Ledge - Non-Surgical Endodontic Retreatment Place a bend of approximately 45 degree in the apical 1-2 mm of a #15 to 25 file. By gentle reciproca

What is the role of stomach in human body, What is the role of Stomach in h...

What is the role of Stomach in human body? The stomach serves both to digest food and to hold it so that it can be gradually released into the small intestine. Food stays in th

Mitosis, What is the role of mitosis in growth?

What is the role of mitosis in growth?

Plant breeding, #questitypes of plant breeding on..

#questitypes of plant breeding on..

Describe the examination of abdomen, Describe the Examination of Abdomen ? ...

Describe the Examination of Abdomen ? The contour of abdomen is to be recorded. Normally the abdomen is scaphold in sharp in a young adult. In case of fullness and bulge presen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd