Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Determine the size and organization of chromosome, Some relatively simple e...

Some relatively simple eukaryotes, like the adder's tongue fern, may have many more chromosomes than a more complex eukaryote, like a mammal. What might this suggest about the size

Anatomy, what is the longest cell in human body

what is the longest cell in human body

What are analogies for a nucleolus, What are analogies for a nucleolus? ...

What are analogies for a nucleolus? If the nucleolus is the president of a factory then the nucleolus is the manager

Ringworm, R i n g w o r m It is also known as dermatophytosis re...

R i n g w o r m It is also known as dermatophytosis resulting in alopecia, scab formation and ring like lesions in the affected body parts. Eti o logy: Zeop

Explain the alkaline copper reduction method, Explain the Alkaline Copper R...

Explain the Alkaline Copper Reduction Method? Since alkaline copper reduction methods are by far the most commonly used methods for blood glucose estimation, let us look at the

Which of the groups is not ionizable, Which of the following groups is NOT ...

Which of the following groups is NOT ionizable? Select one: a. Guanidinium b. Imidazole c. Phosphoryl d. Amine e. Aldehyde

Explain the stages in taxonomic procedures - gamma taxonomy, Explain the st...

Explain the stages in taxonomic procedures - Gamma Taxonomy This phase of taxonomy is also called biological taxonomy and is concerned with the study of speciation. It is inclu

What is chromosome mapping in phenotypic trait, What is Chromosome Mapping ...

What is Chromosome Mapping in phenotypic trait? Chromosome Mapping : By noting how often a gene segment associated with a specific phenotypic trait crossed over during meios

Studying life histories of insects, Studying life histories of insects ...

Studying life histories of insects Cut large rectangular holes in the sides of a big cardboard container and cover them with muslin folded over at the edges and gummed or paste

Diagnostic procedures for acute glomerulonephritis, Diagnostic Procedures ...

Diagnostic Procedures     Taking Clinical history    Urine analysis    Blood sample  for  estimation  of blood urea nitrogen    Antistrepto lysine  (ASO) titer

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd