Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

How cphs produce fermions, How CPHs produce fermions? Answer- For under...

How CPHs produce fermions? Answer- For understanding how CPHs is can produce fermions, do consider to electromagnetic, when the wavelength is growing. It's a CPH So CPH is a ti

What are yeast, Q. What are yeast? Yeasts are unicellular fungi, which ...

Q. What are yeast? Yeasts are unicellular fungi, which are widely distributed in nature. They are somewhat larger than bacteria. The cell length is about 10μm and the diameter

How does phosphocreatine act in the muscle contraction, How does phosphocre...

How does phosphocreatine act in the muscle contraction and relaxation? Phosphocreatine is the main means of energy storage of the muscle cells. During relaxed periods ATP mo

What are the types of proteins in metabolism of lens, What are the types of...

What are the types of proteins in metabolism of lens? Soluble Proteins Its synthesis takes place in the equatorial part on the surface of lens. a. Alpha crystallins

Domestic waste water management, All the water used in the home that goes i...

All the water used in the home that goes into the drain or into sewage collection system is called waste water. Domestic sewage is the waste water from kitchens, bathrooms etc. It

Protozoa phylum, what is the main quality of this phylum

what is the main quality of this phylum

What are neurodegenerative diseases, What are neurodegenerative diseases an...

What are neurodegenerative diseases and how might they be treated? Neurodegenerative diseases that include Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral scler

Find cellular machinery for the replication of nucleic acids, Which of the ...

Which of the following statements is true? Answer Viruses are distinct from cells by the absence of a lipid membrane around them. Viruses are parasites that require the cell's meta

Explain about mental foramen and nerve, Explain about Mental foramen and ne...

Explain about Mental foramen and nerve Mental foramen is a strategically important landmark during osteotomy procedures. Its location and the possibility that an anterior loop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd