Explain procedure for performing the completed coliform test, Biology

Assignment Help:

Explain Procedure for Performing the Completed Coliform Test?

Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise following the steps enumerated herewith.

1. Label the lactose broth tubes and nutrient agar slants with the source of water sample.

2. Inoculate the lactose broth tubes and nutrient agar slants with the isolated coliform colonies on the EMB/Endo agar plates from each of the experimental water samples.

3. Incubate the inoculated broth tubes and slants at 37°C for 24 hours.

4. Examine lactose broth fermentation tubes for production of acid and gas and nutrient slants for gram reaction and cell morphology.

5. Production of acid and gas in inoculated lactose broth and presence of gram negative rods in nutrient slants indicate a positive complete test and confirms the presence of coliforms in water sample.


Related Discussions:- Explain procedure for performing the completed coliform test

Explain some guidelines to help in use drugs wisely, Explain some Guideline...

Explain some Guidelines to Help in Use Drugs Wisely? By now, you are aware that the interaction of foods and drugs is a complex problem. Researchers cannot always predict wheth

Evolution of metazoa, Evolution of Metazoa The sponges, coming under p...

Evolution of Metazoa The sponges, coming under phylum Porifera are the closest to Protista, and can perhaps be regarded even as a colony of protists rather than being multicel

What are interferons, Interferons are small glycoproteins formed by virus-i...

Interferons are small glycoproteins formed by virus-infected cells that inhibit viral infection. They are heterogeneous. Gamma interferons induce MHC class II antigens in macrophag

Bantham and hookers classification systeam, discribe tha bantham and hooker...

discribe tha bantham and hookers systeam of classification

Explain economic status of diet, Economic Status Economic Status  :  I...

Economic Status Economic Status  :  It is one of the important practical considerations to be kept in mind while  formulating  a diet prescription. During  an acute illness, a

Describe structure of biological compounds, Describe Structure of biologica...

Describe Structure of biological compounds? Structure :  The most important chemical compounds in living systems are probably the organic compounds. The term "organic" in the

Cost due to injury to worker, Cost due to Injury to Worker Medical...

Cost due to Injury to Worker Medical cost - medicine, surgery, hospitalization, extended treatment. Working time lost due to injury or death. Time loss of cowork

Describe pathophysiology of tetralogy of fallots, Describe Pathophysiology ...

Describe Pathophysiology of tetralogy of fallots ? The PS causes concentric hypertrophy of right ventricle without enlargement. The right ventricular pressure are equal to or h

Life cycle of malarial parasite, Life cycle of malarial parasite Whe...

Life cycle of malarial parasite When the mosquito sucks the blood, gametocytes enter its digestive system. They migrate into the walls of the digestive system and undergo

Describe the number of implants, Number of Implants The use of more imp...

Number of Implants The use of more implants decreases the number of pontics and the associated mechanics and strains on the prosthesis, and dissipates stresses more effectively

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd