Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

Rehabilitation phase for nutritional treatment -neuro trauma, Rehabilitatio...

Rehabilitation Phase for nutritional treatment - Neuro Trauma? Once the patient stabilizes and starts recovering, be sides nutritional replacement, there is a need to assess fu

How does the odor of flower petals help pollination, How does the odor of f...

How does the odor of flower petals help pollination? The purpose of the perfume is to attract a pollinator - insect, bat, bird or whatever. The reward for the pollinator is a m

Bovine ephemeral fever, B o v i ne ephemeral fever Ephemeral fever ...

B o v i ne ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquit

Explain the endocrine glands involved in the menstrual cycle, What are the ...

What are the endocrine glands involved in the menstrual cycle? What are the hormones in action? The endocrine glands that secrete hormones included in the menstrual cycle are t

Neurons, hpw many neurons do we have?

hpw many neurons do we have?

What is ammensalim, What is ammensalim? Ammensalism is the ecological i...

What is ammensalim? Ammensalism is the ecological interaction in which an individual harms another without obtaining advanatage. Ammensalism is an inharmonious (negative) ecolo

Expalain briefly vitamin A, Vitamin A (Retinol) Purified vitamin A is a...

Vitamin A (Retinol) Purified vitamin A is a viscous yellowish oil. It is freely soluble in most organic solvents such as methanol, ether, acetone, chloroform and petroleum ethe

Guidelines for the family members for healthy coping, Q. Guidelines for the...

Q. Guidelines for the Family Members for Healthy Coping? - Patient and the family members should discuss the situation, change in role,time distribution, share of money and oth

Fungi, is the have 100 nuclus ?

is the have 100 nuclus ?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd