Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

Biochemical, Explain the salient features biochemical origin of life.

Explain the salient features biochemical origin of life.

Reverse osmosis, Ordinary osmosis cannot be used to purify water. If the ap...

Ordinary osmosis cannot be used to purify water. If the applied pressure exceeds osmotic pressure then water starts moving from solution side to pure solvent side. This is reverse

Define the term epimerism, Define the Term Epimerism? In addition to D ...

Define the Term Epimerism? In addition to D and L isomerism sugars also exhibit epimerism. Hexoses that differ from each other as a result of variation of H and OH on carbons 2

Islet of langerhans, Islet of Langerhans: They are insulin-producing tissue...

Islet of Langerhans: They are insulin-producing tissues. It was discovered by Paul Langerhans in 1869. These cells are in groups and look like islands in the pancreas. There are ab

Extracted chlorophyll pigments, Extracted chlorophyll pigments If we...

Extracted chlorophyll pigments If we take chlorophyll molecule in a test tube and provide 2 CO and water and expose the mixture to sunlight, glucose is not formed. If we

Explain demography and demographic transition, Explain Demography, Demograp...

Explain Demography, Demographic Transition and Demographic Cycle We know that population of different countries in the world is always changing. For some countries like India a

Four characteristics of an efficient respiratory surface, State four charac...

State four characteristics of an efficient respiratory surface. Four characteristics of an well-organized respiratory surface are: a) thin epithelium, b) large surface ar

Subcellular particle, Name the subcellular particle where the electron tran...

Name the subcellular particle where the electron transport chain is located. Mitochondria is  the subcellular particle where  the electron transport chain is located.

Physical examination of a child, PHYSICAL EXAMINATION OF A CHILD This ...

PHYSICAL EXAMINATION OF A CHILD This practical as you have seen above deals with assisting in various diagnostic procedures of a child. The first responsibility of you as a

Possible factors contributing to environmental disasters, 1.      The Combi...

1.      The Combination Of Rapid Industrial Growth, Lack Of Regulations, Inadequate Supervision Of Safety Measures By The Authorities And Lack Of Sufficient Opportunities For The L

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd