Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

What are biotic factors, What are biotic factors? The Biotic factors ar...

What are biotic factors? The Biotic factors are living beings (plants, microorganisms and animals) that are part of a given environment.

What is the estimated percentage of water in the human body, Q. What is the...

Q. What is the estimated percentage (in mass) of water in the human body? Is this percentage expected to be larger in the adult or in the old individual? Approximately 65% of t

Calcium channel blockers, Calcium antagonists are not recommended for the t...

Calcium antagonists are not recommended for the treatment of CHF because of their negative inotropic effects. However, second-generation dihydropyridine-type calcium antagonists su

Ovipary, Ovipary, Vivipary and Ovovivipary In each case of external a...

Ovipary, Vivipary and Ovovivipary In each case of external and in some cases of internal fertilisation, development of the fertilized egg (zygote) occurs outside the mother's

Explain use of food additives - method of food preservation, Explain Use of...

Explain Use of food Additives - method of food preservation? Food additives may be defined as substances added intentionally to food, generally, in small quantities to improve

Define about the centrifuge, Define about the Centrifuge? Centrifuge is...

Define about the Centrifuge? Centrifuge is an apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific

Why bacteria classified as prokaryotes, Bacteria have a region known as a n...

Bacteria have a region known as a nucleoid, in which their genetic material is located. Why, then, are bacteria classify as prokaryotes? The nucleoid is not surrounded by a mem

Explain properties related to protein-protein interactions, Explain Propert...

Explain Properties related to protein-protein interactions? Properties related to protein-protein interactions include dough formation, one of the important functional properti

What do you know about vascular access, Q. What do you know about Vascular ...

Q. What do you know about Vascular Access ? Femoral and brachial arteries are still the commonest routes of access for aortography. Thoracic Aorta A sound knowledge of

Which substances transferre from the mother to the fetus, Q. What are the m...

Q. What are the major substances transferred from the mother to the fetus through the placenta? And from the fetus to the mother? From the mother to the fetus the major transfe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd