Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

Do the membranes form only the outer wrapping of cells, Q. Do the membranes...

Q. Do the membranes form only the outer wrapping of cells? Lipid membranes do not form only the outer cover of cells. Cell organelles, such as the Golgi complex, lysosomes, chl

State the method of recombination or crossing-over, Which of the following ...

Which of the following statement is false regarding the method of recombination or crossing-over? A. Crossing-over takes place during prophase I of meiosis B. Recombination

Define interaction of vitamin c with nicotinic acid, Define interaction of ...

Define interaction of vitamin c with Nicotinic acid? NAD, NADP and NMN act as constituents of the hydrogen transferring coenzymes in glycolysis, Kreb's cycle and in the oxidati

Distinguish, distinguish between striated and cardic muscles

distinguish between striated and cardic muscles

Breathing and exchanges of gases , What happens to respiratory system in a ...

What happens to respiratory system in a man going up a hill?

Diagrammatic representation of implant placement, Diagrammatic representati...

Diagrammatic representation of Implant placement Implant placementIt is located directly apical to the tooth it is replacing and not in an embrasure space. The angulati

What is the economic importance of sponges, Q. What is the economic importa...

Q. What is the economic importance of sponges? a few chemical substances secreted by sponges have anti-inflammatory, anti-tumoral activities and antibiotic and they are used in

Etiology, Etiology -Although this approach less desirable, it is useful...

Etiology -Although this approach less desirable, it is useful, short-term option if the etiology of the condition remains unknown. - The patient must inform about what will

What is the importance of vitamin B2, What is the Importance of vitamin b2 ...

What is the Importance of vitamin b2 As a prosthetic group of the flavin enzymes, vitamin B 2 is involved in the reactions of almost all nutrients of plants and animals. They

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd