Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

How ignorance and poor socioeconomic cause pem, How Ignorance and Poor Soci...

How Ignorance and Poor Socioeconomic cause PEM? Status Improper childcare, either as a result of lack of knowledge or lack of time for mother, could also contribute to the onse

Skin, physiology of skin

physiology of skin

Histogenesis and organogenesis, Histogenesis and Organogenesis After t...

Histogenesis and Organogenesis After the octant stage numerous cell divisions occur in various planes. The proembryo become globular or bulb-shaped. As a general rule, some ta

Define proteolytic enzymes, Proteolytic enzymes They catalyze the hydro...

Proteolytic enzymes They catalyze the hydrolysis of peptide bonds in other proteins. In order to prevent them doing generalized damage to all cellular proteins, they are often

What is the process of evolution, Why acquired traits are not directly rela...

Why acquired traits are not directly related to the process of evolution? As acquired traits are not genetically determined, they cannot be passed on to offspring. Therefore,

What information from the patients symptoms, A 16 year old boy named Jake w...

A 16 year old boy named Jake was admitted into the emergency room complaining of headache, fever, stiffness of abdominal muscles and difficulty swallowing. The doctor on staff bega

Metabolic significance of hmp pathway, Metabolic Significance of HMP Pathwa...

Metabolic Significance of HMP Pathway Having gone  through  the HMP pathway, you would have got  some idea about the significance  of this alternative oxidative  pathway for  t

Phylum , what are diploblastic organisms?

what are diploblastic organisms?

Explain suturing - endodontic surgery, Explain Suturing - Endodontic Surger...

Explain Suturing - Endodontic Surgery a. Purpose of suturing: approximation of inside tissues and stabilize the flapped mucoperiosteum b. Major problem of suturing in oral t

Illustrate working of mitochondria and chloroplasts, Q. How is the finding ...

Q. How is the finding of ribosomes inside mitochondria and chloroplasts explained? It is a strong hypothesis that chloroplasts and mitochondria were prokaryotes that associated

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd