Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

Deficiency diseases-sporadic exertional rhabdomyolysis, Sporadic exertiona...

Sporadic exertional  rhabdomyolysis (azoturia, tying up in horses) Azoturia is a metabolic condition of horses that is characterized by reluctance to move and poor performance.

Tricuspid valve - heart, It is made up of three cusps, anterior, posterior ...

It is made up of three cusps, anterior, posterior and septal. The septal cusp is attached to the medial margin. The anterior cusp is attached to the superior-lateral part of the ma

Carbohydrate is an organic compound , The Carbohydrate sets are covalently ...

The Carbohydrate sets are covalently attached to various variant proteins to form glycoproteins. Carbohydrates are a shorter percentage of the weight of glycoproteins than of prote

Name the implant failures, Implant failures On the basis of the stage w...

Implant failures On the basis of the stage when problems occurs implant failures can be classified as : i) Before Abutment Connection. ii) During Abutment Connection.

VASCULAR PLANTS., WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

Light microscopy, LIGHT MICROSCOPY (LM) A study of the structure of organ...

LIGHT MICROSCOPY (LM) A study of the structure of organisms is an essential requirement of biology. However, unaided human eye cannot see objects smaller than 0.1 mrn(100 p) in d

Degrading monoglyceride, Degrade a monoglyceride that has an 18-carbon fatt...

Degrade a monoglyceride that has an 18-carbon fatty acid attached to it by Ester bonds. You will have to degrade the glycerol component followed by the fatty acid in presence of O2

Quantitative changes, Quantitative changes The quantitative changes in...

Quantitative changes The quantitative changes in the availability of the active biomolecules occur in order to compensate for the loss of efficiency of the system caused by th

Explain gellan gum, Explain Gellan Gum Gellan is an exopolysaccharide c...

Explain Gellan Gum Gellan is an exopolysaccharide consisting of  β-D-glucose,  β-D-glucuronic acid,  α-L-rhamnose with acetate and glycerate groups, produced by the bacterium S

What is the gross primary production of an ecosystem, What is the gross pri...

What is the gross primary production of an ecosystem? How does GPP relate to photosynthesis? Gross primary production of an ecosystem, or GPP, is the amount of organic material

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd