Procedures for diagnosis - restriction enzyme analysis, Biology

Assignment Help:

Restriction enzyme analysis:

The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study of viruses. Restriction endonucleases cut DNA molecule at specific sequences to produce a reproducible number of fragments in agarose gel electrophoresis for each DNA genome. The number and size of the fragments generated depend on the enzyme used and the frequency with which the specific cleavage sites are present in the DNA. This technique allows differentiation between the variants of the organisms and is a powerful tool for the study of infectious agents.


Related Discussions:- Procedures for diagnosis - restriction enzyme analysis

Describe how platelets interact to form a blood clot, Describe briefly how...

Describe briefly how platelets, fibrin and red cells interact to form a blood clot.   Platelets release a substance which, indirectly, causes fibrinogen to be changed to fi

Define common complications with spinal trauma patients, Define Common comp...

Define Common complications with spinal trauma patients? Common complications with spinal trauma patients include pressure sores or decubitus ulcers, hypercalciuria and renal s

Plant responses to light-dark cycles, Plant Responses to Light-Dark Cycles ...

Plant Responses to Light-Dark Cycles Based on their requirement for day length (number of hours of light) for flowering plants have been grouped under three major categories,

Nutrition, definition of dietary factors. or what are dietary factors?

definition of dietary factors. or what are dietary factors?

Which part of heart serves as an effector, Which of the following serves as...

Which of the following serves as an effector, or as part of an effector, that functions in a negative feedback system? A. Nicotinic Acetylcholine Receptors (nAChR) in SA node c

How many biochemicals would be detected, How many biochemicals would be det...

How many biochemicals would be detected in the stomach contents of an omnivorous dinosaur?

What are the major cellular features of fungi, Q. What are the major cellul...

Q. What are the major cellular features of fungi? There are pluricellular and unicellular fungi. All fungi are heterotrophs and eukaryotes. Fungi have cells with cell wall m

Polyarthritis, It is the most common (occurring in 75 per cent cases of ARF...

It is the most common (occurring in 75 per cent cases of ARF) manifestation of ARF. It involves large joints, it is typically fleeting in character shifting from one large joint t

Objective of the radiographic technique, Objective of the Radiographic Tech...

Objective of the Radiographic Technique The ball bearings are opaque and their diameter have to be measured with the help of vernier caliper prior to use. (2 to 5 mm diameter b

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd