Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Define the structure of vitamins, Define the Structure of Vitamins? Fo...

Define the Structure of Vitamins? For your convenience, however, we have given the structures of these vitamins here in the text. Look at Figure which illustrates the fat-solu

Restructured meat products, Re s t r u c t u re d Meat Products ...

Re s t r u c t u re d Meat Products Restructuring technique is used for developing convenience products with texture in between intact steak and a comminuted product.

Help, #Predict: In order to be able to energize a transport protein by phos...

#Predict: In order to be able to energize a transport protein by phosphorylation (by addition of a phosphate group from ATP), the portion of the protein to which the phosphate grou

Seismic waves, Earthquake creates seismic waves that travel through and aro...

Earthquake creates seismic waves that travel through and around the surface of the earth. Seismic waves are the waves of energy caused by sudden breaking of rocks during an earthqu

What organisms make glycogen, What organisms make starch? What is it used f...

What organisms make starch? What is it used for? What organisms make glycogen? What is it used for?

After the morula stage what is the next stage, Q. After the morula stage wh...

Q. After the morula stage what is the next stage? What is the morphological feature that defines this stage? In the blastula stage the compactness is lost and an internal cavit

Define endurance capacity by standard running tests, Define Endurance capac...

Define Endurance capacity by standard running tests? Endurance capacity, on the other hand, is measured by standard running tests in which time to exhaustion is taken as the en

Symptoms of enteropathogenic escherichia coli, Symptoms: The E. coli gastro...

Symptoms: The E. coli gastroenteritis syndrome is caused by the ingestion of 10 6 -10 10 viable cells/g that must colonize the small intestine and produce  enterotoxin. The syndr

Instrument for measurement of absorbed radiation-colorimeter, Define Instru...

Define Instrument for measurement of absorbed radiation-Colorimeter? The simplest types of, photometric instruments are designed for measurements in the visible region of spect

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd