Explain methods of protein estimation, Biology

Assignment Help:

Explain Methods of Protein Estimation?

Several methods for protein estimation are available. These methods are based on several analytical characteristics of the protein. Laboratory estimation of protein is based on any of the following principles:

i) Nitrogen estimation based on the principle of Kjeldahl's method,

ii) Estimation of tyrosine in protein (as in Lowry's method) or both phenyl alanine and tyrosine in protein (Folin's method).

iii) Biuret method - most commonly used method in clinical practice and teaching laboratories.

We have studied some of the tests in qualitative methods and the principle is the same. The Biuret method for example can be used for both qualitative and quantitative estimation of proteins. Let us learn about these methods in details. We shall, however, focus on the kjeldahl's method and the biuret method here in this section.


Related Discussions:- Explain methods of protein estimation

Define the prevention of diseases - function of vitamin e, Define the Preve...

Define the Prevention of diseases - Function of Vitamin E? Because of its anti-oxidant function and its role in inhibiting cell proliferation of smooth muscles, vitamin E can b

General zoology, please help me with the general characteristics of the fol...

please help me with the general characteristics of the following classes:1.mammalia,2.ostichthyes,3.cyclostomata,4chondrichthyes,5.amphibia,6.reptilia

Show germ layer from which the coeloms originate, Q. What is the germ layer...

Q. What is the germ layer from which the coeloms originate? The coeloms are deriving from mesoderm.

Imaginal discs, Imaginal Discs In the holometabolous larva, there are ...

Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th

Explain reversion flavor, Reversion flavor:  The rapid oxidation of br...

Reversion flavor:  The rapid oxidation of branched furan fatty acids on exposure to light, leads to the production of an intensive aromatic substance, which along with diacety

Explain about pantothenic acid, Pantothenic acid (Calcium pantothenate) ...

Pantothenic acid (Calcium pantothenate) Calcium pantothenate is a white, loose, faintly hygroscopic powder without odour and of bitter taste. It is easily soluble in water, gly

Classification of different agro-industrial by-products, Classification of ...

Classification of Different Agro-Industrial By-Products The agro-industrial by-products can be grouped according to the content of nutrients namely: (i) Energy rich supplem

Explain the urinary system in human body, Explain the Urinary System in hum...

Explain the Urinary System in human body? The urinary system removes the waste materials produced as a result of all the metabolic process required to sustain life. The process

Excretory system, name of the excretory organ of a lizard

name of the excretory organ of a lizard

Define the wealth care delivery, Define the Wealth Care Delivery ? The ...

Define the Wealth Care Delivery ? The challenge that exists today in many countries is to reach the whole population with adequate health case services and to ensure their util

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd