Ethidium bromide, Biology

Assignment Help:

Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.


Related Discussions:- Ethidium bromide

Explain the procedure for morphological study of fungi, Explain the Procedu...

Explain the Procedure for Morphological Study of Fungi? Now carry out the exercise, following the steps enumerated herewith: 1. Prepare a humid chamber by placing two-three

Hypothesis be used to predict the outcome of future test, A hypothesis can ...

A hypothesis can be used to predict the outcome of future testing. Which type of hypothesis is easier to test? A. It is easier to specify and test for a difference between two outc

Antimicrobial prophylaxis - number of doses, Timing and number of doses ...

Timing and number of doses It has been common practice to give antibiotics at the time of anesthesia induction, which results in adequate serum and tissue levels of the drugs;

Pancreas and its structure, PANCREA S - It is derived from the endoder...

PANCREA S - It is derived from the endoderm of the embryo. Structure . The pancreas lies inferior to the stomach in a bend of the duodenum. It is both an exocrine an

Horse diseases-horse-pox, Horse-pox Horse pox is a benign disease chara...

Horse-pox Horse pox is a benign disease characterized by the typical pox lesions on the lower limbs or around the muzzle and buccal mucosa. This is a rare disease, caused by a

Ciliates - parasitic protozoan, Ciliates - Parasitic Protozoan The onl...

Ciliates - Parasitic Protozoan The only ciliate of medical and veterinary importance is Balantidium coli which inhabit the large intestine of man, apes, monkeys and pigs. B. c

Changes in the ions, Changes in the Ions The concentration o...

Changes in the Ions The concentration of potassium, phosphate and nitrate declined significantly in the bathing medium within four days. The concentration of so

Explain the anthropometric measurements, Anthropometric measurements An...

Anthropometric measurements Anthropometry is the study of the measurement of the human body in terms of the dimensions of bone, muscle, and adipose (fat) tissue. Anthropometric

Explain the scaling from individuals to ecosystems, Explain the Scaling fro...

Explain the Scaling from individuals to Ecosystems? Models that explain how individual organisms acquire energy and materials, and how they use them for survival, growth and re

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd