Ethidium bromide, Biology

Assignment Help:

Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.


Related Discussions:- Ethidium bromide

Haploid production, Haploid Production The higher plants are normally ...

Haploid Production The higher plants are normally diploid, with two sets of chromosomes in their somatic cells. Their haploids (with one set of chromosomes) arise in nature by

Coelom, Why Chordates are Schizocoelic, while other deuterostomes are enter...

Why Chordates are Schizocoelic, while other deuterostomes are enterocoelic?

What do you mean by monographs, Q. What do you mean by Monographs? A mo...

Q. What do you mean by Monographs? A monograph is defined as "the complete account as can be made at a given time of any one family, tribe or genus, nothing being neglected of

Pollenkitt - pollen wall, Pollenkitt - Pollen Wall The lipoidal layer ...

Pollenkitt - Pollen Wall The lipoidal layer found on the outside of the mature pollen grains of many insect pollinated species is known as the pollenkitt. In addition to lipid

Neural basis of responses to sex pheromones, Neural Basis of Responses to S...

Neural Basis of Responses to Sex Pheromones The ability of insects to detect and discriminate between a variety of pheromones with great sensitivity depends on the presence of

Define the healing process - root canal treatment, Define the Healing Proce...

Define the Healing Process - Root Canal Treatment The course of healing varies and depends on: 1)  Initial size of the lesion: It takes longer for larger periradicular lesio

Define the first step to envision the pattern of deformation, Define the fi...

Define the first step to envision the pattern of deformation. The first step in the analysis is to envision the pattern of deformation that would accompany such a failure. In t

Explain precautions for performing completed coliform test, Explain Precaut...

Explain Precautions for Performing the Completed Coliform Test 1. Aseptic conditions should be adopted while performing the experiment. 2. Destaining is the critical step in

Describe the terms – cilia, Cilia and flagella share a common structure, wi...

Cilia and flagella share a common structure, with a microtubular core which has a 9+2 organization with nine paired doublets of microtubules surrounding a central pair to form the

A disulfide bond can form between any two amino acids, A disulfide bond can...

A disulfide bond can form between any two amino acids that have sulfur in their side-chain.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd