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Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.
Classification
What is sustainable agriculture? Describe the contribution of biopesticides and biofertilisers in sustainable agriculture.
While explaining the concept of natural selection, we have stressed the fact that natural selection is synonymous with differential or non-random reproduction. Natural selection ai
Consider the following four statements (a - d) regarding kidney transplant and select the two correct ones out of these. 1. Even if a kidney transplant is proper the recipient m
Q. Show the pH range of natural honey? The pH of natural honey ranges from 3.4 to 6.1. Acidity of honey is primarily due to presence of acids such as gluconic acid, pyruvic a
What is Atraumatic needles Atraumatic needles with sutures comprise an eyeless needle attached to a specific length of suture thread. There are several shapes of surgical n
In the process of tissue culture in plants, what is required to induce the formation of a complete plant, in addition to a growth medium with nutrients? The appropriate p
Describe the Technique of Operation in prosthetic valve endocarditis? The operative principle is drainage of abscess, removal of debris and valve rep or replacement to reverse
What are the respective functions of the separation of homologous chromosomes and of the separation of identical chromatids in meiosis? The separation of homologous chromosomes
FLUORIDE It is mostly available in drinking water. Fluoride is essential for the formation of enamel of the teeth. Deficiency of fluoride causes weakness of enamel.
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