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Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visualize RNA or DNA in the agarose gels placed on the UV light boxes. Proper precautions are needed, because the ethidium bromide is extremely mutagenic and the UV light damaging to eyes. Ethidium bromide is also involved in cesium chloride gradients during the process of ultracentrifugation, to separate the supercoiled circular DNA from linear and the relaxed circular DNA.
Explain about the Invert sugar? Invert sugar is sucrose, which can be hydrolysed to split the disaccharide into its component sugars, fructose and glucose. It is known as inver
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Fructose-1,6-bisphosphate and Fructose-6-phosphate : The conversion of fructose-1,6- bisphosphate to fructose-6-phosphate is catalysed by fluctose-1,6-bisphosphatase which i
Eye complications are very common in patients with diabetes. In fact, diabetes related eye diseases may not produce symptoms for a very long time and hence, it remains undetected t
A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer t
Science as a human endeavour: Science is a human endeavour. Human beings, from prehistoric times, attempted to control nature for their own welfare. For this, they had to ob
How Infections and Infestations cause PEM? Childhood infections (viral/bacterial) and parasitic infestations are almost always associated with PEM. These cause anorexia (loss o
compare and contrast plant cell and animal cell with labeling diagrams
What two biotic factors could affect an antelope living in the Serengeti? The biotic factors that may affect an antelope living in the Serengeti could be:predation by carnivore
A few statements about the mechanism of evolution are made. Mark the correct statements. (i) Evolution happens at the individual level as well as population level. (ii
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