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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
Vitamin a deficiency leads to a variety of disorders of the eyes and this effects the vision. Some of the disorders due to vitamin A deficiency are given below. 1) Night Blindne
Which is the type of gamete (for a given trait) produced by a dominant homozygous individual? What is the genotypical proportion of these gametes? What about a recessive homozygous
Cell-mediated immunity: The immune reaction directed against the body cells which have been infected by viruses and bacteria; and are controlled by T cells.
“Define nosocomial infections. Discuss the factors that might influence whether a patient may acquire nosocomial infection. How might these risks be reduced?”
Simple interrupted technique of Suturing The simple loop is the most commonly used technique in dentistry and is routinely used to adapt tension free, mobile surgical flaps.
Describe about diagnostic approach for congenital heart disease ? In this section we propose to outline the principles of a diagnostic approach that is applicable to newborns,
Q. Describe Postpartum Cardiomyopathy? It is defined as presentation of LV Systolic dysfunction and heart failure in last trimester of pregnancy or within 6 months of delivery.
Viscosity and Plasticity of colloidal particle Various degrees of viscosity and plasticity are encountered in colloids. Viscosity may be described as resistance to pouring.
The ph of urine can be quite variable. Why? Why is bromothymol blue used as a ph indicator instead of phenal red when titrating blood?
Adaptations to high wind velocity The mechanical force of the wind and the grinding action of sand, dust, snow and other materials driven by it cause the plants to adapt themse
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