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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions mutations or any mismatches. First the pcr is utilized to intensify the normal and mutant allele of targeted sequence, these pcr primers are labeled with fam(6-carboxyfluoroscein amidite) which is a blue fluorescent dye and reverse primer with tet(4,7,2,7-tetra chloro-6-carboxy fluoroscein) amidite which is a green fluorescent dye.
Denaturation and renaturation of normal and mutant allele in a mixture is made, consequently mismatch heteroduplexes will form about 50 % of the time, each and every base pair creates two mismatch. The endo vii enzymes then scan the double stranded dna until it finds structural distortion, either a bubble made by single base pair mutations or a hetero duplex loop created by hybridization of a wild type allele with a mutant allele having an insertion or deletion. The enzyme cleaves with in 6 base pair on 3"side of mutation creating two smaller fragments, one blue and the other one green. The dna sequence is analyzed on automated dna sequence and mobility of every fragment is analyzed.
If the chemical formula of a substance is C17 H 31 COOH, what can you tell about the identity and amounts of elements in the molecule? Can someone please explain me this?
Determine the Urine analysis Urine constituent may be altered in urinary tract infection, systemic disease. However it is not routinely prescribed for most of the potential imp
Accidents Resulting in Death Accidents that cause death are dealt with in a different way. The law requires full compensation to the widow for complete life or until remarriag
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Agrostology : This is related to all types of grasses. Agrostology is sometimes called as graminology. Agrostology is the scientific study of grasses. It typically encompasses the
EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES
How to draw diagrams of bones
Q. Management of hypertension? the management of hypertension, angina pectoris and myocardial infarction. It must be clear to you that some aspects of dietary management may di
what are carbohydrates
Deforestation - Degradation of Ecosystem The deforestation is a broad term. It means the removal/destruction of the forest cover or the vegetation of an area. It includes repe
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