Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
Nature: Matter and Energy Nature is not just our immediate surrounding or environment, but is the whole Universe of Cosmos. Study of the origin and topography of cosmos is ca
Define observation or inference for barfoed test? 1. Reddish brown precipitate is seen on the sides and bottom of the tube. The precipitate of the sides and bottom indicates th
Which of the following is true in a skeletal muscle? A. Movement of the cross-bridge occurs only when the myosin head is detached from the actin molecule. B. The binding of
State the term Autopsy data - Brain patient Autopsy data are not always entirely usable for validation purposes, in that numerous changes may have taken place in the patient's
what is usually regarded as evidence that photosynthesis has occurred in a plant?
site of fertilistion in human
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Define effect Micronutrients during pregnancy period? The need for many vitamins and minerals is increased during pregnancy. Since energy intake increases, the requirements for
Q. What is the homeostasis? What are the sensors, effectors and controllers of homeostasis? Homeostasis comprises the processes by which the organism maintains extracellular an
Exercise- Heat Production During physical activity heat production by exercise can to some extent substitute for heat generated by shivering. However, exercise also tends to
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd