Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
Explain the Regulation of Blood Glucose Concentration? A number of mechanisms function to maintain blood glucose at remarkably constant level of 70-100 mg/dl under fasting cond
Botulism Botulism is a rapidly fatal motor paralysis. It is caused by ingestion of toxin of Clostridium botulinum in food. Many species of domesticated, wild animals and birds
a) Explain somatic hybridisation? b) Describe the various steps involved in the process. Mention any two uses of somatic hybridisation
How is reproduction done in beings of the class Reptilia? These beings reproduce sexually by internal fecundation by means of copulation among male and female individuals. They
Anomalies Related to Oral Cavity: Under these anomalies we will briefly discuss the cleft lip and cleft palate. You must have seen and/or nursed a baby born with a cut on
Define etiological risk factors in cancer - Genetic Factors? Some cancers are programmed by genes to develop. Gene mutations result from one or more regulatory genes in the cel
Problem 1: Which are the best procedure to adverse events of a given marketed Drug product? Mention methodology / procedure to detect adverse events of a given marketed pro
Question 1 What are dimorphic fungi? List the clinical manifestations produced by various dimorphic fungi. Add a note on isolation and identification of various dimorphic fungi
What is the cost-benefit relationship regarding sewage treatment as a method to fight water pollution? To treat sewage is much cheaper for society. The non treated sewage pollu
Q. List the goals of management of Type 1 Diabetes Mellitus? Goals of management of Type 1 Diabetes Mellitus: a) to keep blood sugar level as close to normal as possible.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd