Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
before stem cuttings are planted the cut end of the stem is often dipped in a hormone powder .what is the point of this?
how do we write a limerick spell?
Petrol or gasoline can be synthesized by following three methods: 1. Polymerisation 2. Fischer - tropsch method 3. Bergius process polymerisation In
Difference between Sympathetic and Parasympathetic Nervous System - S.NO . SYM P A THETIC PERASYM P A THETIC 1.
Q. Is fecundation in amphibians internal or external? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species f
Why plant nutrients said to be essential An element is said to be essential if, (i) The deficiency of an element in plant, makes it impossible to complete the vegetative
Q. How Occlusal load affect osseointegration? Overloading the recognizing bone tissue prematurely will cause failure of osseointegration. A two stage surgery is advised routine
Explain in details Aerobic Respiration? The pyruvate produced in glycolysis is further broken down in most organisms. This process, called aerobic respiration, requires gaseou
State the phases of soil genesis Soil genesis embodies two distinct phases. One is weathering that involves disintegration and decomposition of rocks and minerals, the other is
Define Calamity and Emergency Management - nutritional needs of humans? During past many years, we have seen, as well as, realized -that natural calamities strikes countries, b
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd