Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
Explain the senses taste and smell ? The pigmented cone cells of the retina transmit information to bipolar nerve cells, then to ganglion cells, and on to the optic nerve and t
why do ecological models commonly have limited applications?
Define briefly about the Conjugated Proteins? Conjugated proteins are made up of simple proteins combined with a non- proteinous substance. The non-proteinous substance is know
Define Protection of poly unsaturated fatty acids (PUFA) from oxidative damage? The major biological role of vitamin E is to protect PUFAs and other components of cell membrane
There are three methods of collections: 1. Kerbside collection: In this collection system the refuse is brought in containers and placed on the footway, from where it is
Phanerophytes - Classes of Life Form The perennating buds in this case are present on erect, negatively geotropic shoots, much above the ground. These buds are naked or least
Providing Critical Resources - Conservation of Wildlife Another way to improve the habitat of a threatened species is to determine which resource is limiting the population si
Social Determinants of Health - Transport Rising worldwide incomes have led to a massive use of automobiles and in particular cars. Cars are seen as a threat to health because
Composition of agar Agar we learnt consists of a mixture of agrose and agropectin. Agrose has a linear polymer structure consisting of alternating D-galactose and 3,6-anhydro
Define about the Column chromatography? In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The gener
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd