Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
What are holandric genes? Holandric genes are genes situated in the nonhomologous region of the Y chromosome. Holandric genes condition phenotypes that emerge only in men as in
where are flame cells present?
what is the food getting habits of phylum coelenterata?
Describe the structure of pectin Its structure is presented in the figures (a) and (b). Pectin is a polygalacturonic acid ester along with rhamnose, arabinose and galactose.
what is the bioindicator of water pollution.
Silver point extended below canal orifice - Ultra-sonic tip - Microtube tape: The post removal system (PRS) Trephine bur Masserann kit - Do trimming by
Define briefly about the Cereal Proteins? Cereal grains, properly ripened and dried for optimum storage stability, have protein content ranging from 6-20%. Proteins are found i
Endocytosis is the uptake of extracellular macromolecules across the plasma membrane into the cell. The objects to be ingested is progressively enclosed by a small portion of
Describe about Acyanotic Congenital Heart Disease ? The following lesions will be discussed here: Left to right shunts: Atrial Septal Defect (ASD), Ventricular Septal Defect
Describe in brief about Pseudoisoehromatic Plates test Commonly known as Ishihara's plates, these reveal one pattern to the normal eye and another to the colour deficient. Colo
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd