Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
What are synthetic auxins and what are their uses? Synthetic auxins, like indolebutyric acid (IBA) and naphthalenic acid (NAA) are substances same to IAA (a natural auxin) but
What is the difference between asexual and sexual reproduction
Q. What is the difference between tracheophytes and bryophytes? Bryophytes are nonvascular plants (liverworts, mosses, hornworts), that is they do not have a conductive system
Define Excretion of Methyl malonic acid in urine (Methyl malonil aciduria)? Methyl malonic acid is found only in traces in normal urine (1-2 mg/day). In vitamin B 12 deficie
Q. How is the yolk sac formed? What is the function of the yolk sac? The yolk sac is formed from the covering of the vitellus by few cells originated from the primitive gut.
How Sugar is used in Custards? While flour protein forms the structure of baked goods, custards are composed of egg protein structures. If the egg white solidifies too early al
A study set out to determine whether tetrahydrocannabinol (THC, the psychoactive component of marijuana) affects fertility in male hamsters. Two different experiments were conducte
Photosynthesis Two main energy pathways are recognised in living systems. These are photosynthesis and cellular respiration. Both have their respective but different electron
Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal
What are the six criteria used to build a complete evolutionary branch of vertebrates? Dichotomy in each of the six following criteria builds the vertebrate evolutionary branch
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd