Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
What are the typical vegetation and the typical fauna of the temperate forests? In the temperate forest deciduous trees predominate. Various Mammals are found in great number,
NEURITE S - The processes of neurons are called neurites. These are of two types: dendrites or dendrons and an axon or axis cylinder or neuraxon. (a) Dendrites (Den
Cytoplasmic inclusions are cytoplasmic molecular aggregates, like as pigments, organic polymers and crystals. They are not considered cell organelles. Fat drops and glycogen gra
Why is it important for chromosomes to be condensed during mitosis and decondensed during interphase? During mitosis the major problem to be solved is the correct separation of
advantages of spread plate method over pour plate method
Q. Explain False Positive Tests? As ST depression has been equated with CAD, patients with this finding who have less than a critical coronary narrowing has been called false p
Intracellular Digestion We all know that unicellular organisms do not have a separate alimentary canal system. All the functions of life are carried out inside a single cell.
How can proteins be classified? Give suitable examples. Proteins can be classified based on: Shape and size: fibrous or globular proteins. Functional properties: I
Define observation or inference for picric acid test? 1. A mahogany red colour will be seen. The mahogany red colour indicates the presence of reducing sugar. All monosaccharid
explain briefly about ICZN
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd