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Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that controls seed dormancy and environmental stress responses. The protein is known to belong to a class of enzymes known as p450s of which there are 500 different genes in Arabidopsis, the model plant system. The company isolates a full length cDNA for each gene using PCR with primerscontaining attB sequences and then each individually cloned into pDONR/Zeo (from Invitrogen) via a BP reaction for all 500 different p450 genes from Arabidopsis (the cDNAs vary in length from 1.5-2.5 kb). Next the company proposes to express each gene in yeast, and then carry out an ABA-8 hydroxylase reaction in vivo to determine which cDNA encodes ABA 8-hydroxylase activity.
Outline a cloning strategy(s) (experimental procedure) the company could take to express all these genes in yeast - a simple flow diagram detailing the steps is all that is needed. Design a vector(s) that would be central in this strategy (can just draw by hand) and list all the important features that would be required (i.e what cloning sites or antibiotic resistance genes would be required if any) but not any features that would be excess to what is required.
Which are the phases of the menstrual cycle? The menstrual cycle is separated into two main phases: the follicular (or menstrual) phase and the luteal (or secretory) phase.
Biochemical Function - Essential Elements Elements Mg, Mn, K, Ca and Fe are cofactors for many enzymatic reactions. Fe is carrier of electrons in electron transfer chain. Phos
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What is crossing over? How is meiosis related to this phenomenon? Linked alleles, for instance, A-b and a-B, form the gametes A-b and a-B that maintain the linkage of the allel
Conventional agro-industrial oilseed cakes Groundnut meal: It is readily available protein source byproduct from the extraction of groundnut, which is a popular human food i
Q. Explain about Catheters and Guide Wires? The commonly used guide wires vary in diameter from 0.012 to 0.052; with 0.035 or 0.038 being the most commonly used sizes. The stan
virus symmetry
Maedi/visna Maedi, a progressive pneumonia, and visna, a neurotropic disease, found in sheep and goats are caused by the Maedi/visna virus, belonging to the family Retroviridae
SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat
Transport system in higher animals The circulatory system in higher animals has undergone several changes during evolution. The heart has become totally muscular. It p
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