Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Design of expression vectors
Expressing a transgene in an appropriate manner is a huge task. The gene construct that is quite active in transfected cells may be only weakly expressed in transgenic animals. Moreover, each line of animals shows a specific pattern of transgene expression. In addition, a number of transgenes remain silent in some cells but are expressed in others. This is a clear limitation for the use of transgenic animals. A large number of transgenic founders are attempted until they find the lines expressing the foreign gene as they expected. This approach implies wastage of time and animals but it appears sufficient to obtain 1,000-10,000 copies of recombinant protein per cell exhibiting the expected biological action. This relative but real success of the expression vectors dissuaded researchers for years from improving them.
The situation is altogether different when recombinant proteins are to be prepared in milk. Highly efficient expression vectors are often needed to obtain the expected large amounts of proteins recovery from milk. However, it must be ensured that the recombinant proteins are restricted to milk as much as possible to avoid any potential deleterious effects on host animals. A significant amount of work was done for the production of recombinant proteins in milk and a few efficient expression vectors were designed and patented. Yet basic studies on the mechanisms of gene expression and empirical observations have led to defined rules for vector construction and to identification of pitfalls that can be avoided. A functional transcription unit is composed of several elements that cooperate with each other. These elements and their mode of action are not yet fully known. A gene construction consists of associating more or less known elements. This may generate nonfunctional vectors for unknown reasons. The major recommendations for designing reliable vectors may be summarized as follows.
Secretion of Hormones The secretion of most hormones (except steroid) is by the process of exocytosis. Figure summarises the formation, transport, release and reconstitution o
Economic importance of phylum mollusca
RECORDS AND REPORTS: The principles of administration are described as 'POSDCORB'. The 'R' stands for recording and reporting. Recording and reporting is related to all oth
Define Protein Requirement for Cancer Patients? Both the metabolic stress of cancer, as well as, chemotherapy results in increased tissue catabolism. Hypoalbuminemia and anaemi
Q. Describe the Discovery of the Cell? Ans: The discovery that living organisms are composed of cells was made by an Englishman, Robert Hooke, in 1665. Hooke used the light mic
Define Effect of feeding method on drug availability? The form in which a drug is administered or enters the body can influence its absorption, metabolism or excretion. This be
Bone Remodeling Bone remodeling differs from the other means of bone structure alteration in that osteoblasts and Osteoclasts do not act independently but are coupled and bone
Does the trend of the change in shape of the graph as temperature increases differ when a different gas is examined?
Calculate the Level of Magnesium in urine? Urinary Mg excretion decreases with Mg deficiency. Therefore, Mg excretion before and after administration of an intravenous Mg load
How do antibody-based tests detect how HIV infection works? After the infection by the HIV the immune system starts the production of antibodies (primary immune response) again
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd