Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Which is better to grow plants in rock sand or soil, Which is better to gro...

Which is better to grow plants in Rock sand or soil? When we grew plants inside, with there is no wind, and the plants in the rocks grew improved than the plants that were grow

Definition of nursing process, Definition of Nursing Process According...

Definition of Nursing Process According to WHO (2977) the "nursing process" is a term applied to a system of characteristic nursing interventions in the health of indivi

Amoeboid protozoan, Amoeboid Protozoan Amoeboid protozoan are distingu...

Amoeboid Protozoan Amoeboid protozoan are distinguished by the presence of flowing extensions of their body known as pseudopodia. These are used for feeding and locomotion. Th

Growth and development, what is the clear explanation of growth and develop...

what is the clear explanation of growth and development

Illustrate morphological evidence, Q. Illustrate Morphological Evidence? ...

Q. Illustrate Morphological Evidence? Morphology is the study of structure and form of plants and animals usually dealing with the organism and its component organs. Morphologi

What are primary indications for pharmacologic stress, Q. What are primary ...

Q. What are primary indications for pharmacologic stress? The primary indications for pharmacologic stress are inability to exercise or the desire to identify viable myocardium

Explain the control of air pollution, Explain the Control of Air Pollution ...

Explain the Control of Air Pollution a. Air belongs to everyone Need it to treat air pollution as public problem   b. Air pollution is an inevitable concomitan

Explain about the primary protein derivatives, Explain about the primary pr...

Explain about the primary protein derivatives? a) Proteins: These are the insoluble products which result from the incipient action of very dilute acids or enzymes. e.g. casein

State the branemark theory of osseointegration, Branemark's Theory of Osseo...

Branemark's Theory of Osseointegration In 1952, Swedish orthopedic surgeon, Branemark, conducted microscopic studies done in situ of the bone marrow in rabbit's fibula with in

Structure and content of halsted reitan battery, Structure and Content of ...

Structure and Content of Halsted Reitan battery Although there are several versions of the Halsted Reitan battery, the differences tend to be minor, and there appears to be a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd