Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Influence of lv function, Influence of LV Function :  LV functions, whethe...

Influence of LV Function :  LV functions, whether normal or impaired is important while selecting a patient for CABG and for long-term results of surgical revascularisation. If le

Determine the term - angiomas, Determine the term - Angiomas Angiomas a...

Determine the term - Angiomas Angiomas are congenital collections of abnormal vessels that divert the normal flow of blood. These capillaries, venous, or arteriovenous (A-V) ma

Explain the periodontal consideration, Explain the Periodontal consideratio...

Explain the Periodontal consideration Periodontal consideration would include: Hard Calculus Build up:   Any index assessing the amount of calculus build up can be used .

Classification of microorganisms basis of temperature, Classification of Mi...

Classification of Microorganisms On basis of temperature for growth  Microorganisms can be classified into: -  Thermophillic: Microbes those require high temperature for

Retention of soil moisture, Retention of Soil Moisture The movement of ...

Retention of Soil Moisture The movement of water into and within the soil, moisture storing capacity of soils and the availability of moisture to plants are governed by soil pr

Functional principles of excretory organs, Normal 0 false fal...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is agar-agar, What is agar-agar? Agar-agar is a sulphonated mucopo...

What is agar-agar? Agar-agar is a sulphonated mucopolysaccharide containing mainly D-galactose, D-glucuronic acid and 3,6 anhydro L-galactose. It is derived from red sea weed e

Explain tuberculosis, Tuberculosis Tuberculosis is a chronic infectious...

Tuberculosis Tuberculosis is a chronic infectious disease which is caused by a bacterium - Mycobacterium tuberculosis.  It affects the lungs most commonly but call gel localize

How is the skin involved in the regulation of temperature, Q. How is the sk...

Q. How is the skin involved in the regulation of body temperature? Skin is one of various organ systems participating in maintaining a core temperature, meaning the temperature

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd