Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Explain about harvesting autogenous bone grafts, What are the precautions t...

What are the precautions to be taken when harvesting autogenous bone grafts from mandibular symphysis and ramus? When harvesting grafts from the mandibular symphysis region, fo

Private or voluntary health insurance schemes, Normal 0 false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Symptoms of ulcerative colitis, Q. Symptoms of ulcerative colitis? As d...

Q. Symptoms of ulcerative colitis? As discussed in the case study above, the common symptoms are: 1. Mild abdominaI discomfort, an urgent need to defecate several times a da

Skeletal biology, What is Skeletal Biology? What is specification of Skelet...

What is Skeletal Biology? What is specification of Skeletal Biology? Describe please.

What do you mean by oesophagitis, Q. What do you mean by Oesophagitis? ...

Q. What do you mean by Oesophagitis? We already know that oesophagus is a muscular tube 25 cm in length and basically helps in transporting the food from the mouth to stomach,

Can enhancers work in trans, Can enhancers work in trans? That is, can an e...

Can enhancers work in trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA. Propose an experiment to test this. Be sure to discuss any ap

Explain various chemical formulas, Explain various chemical formulas? C...

Explain various chemical formulas? Chemical Formulas A molecule can be represented in any of three ways: its chemical formula, structural formula, or Lewis diagram. A chemic

What is ventilation, What is ventilation   A.  An increase in the hydro...

What is ventilation   A.  An increase in the hydrogen ion concentration in the interstitial spaces of the  brain stem leads to an increase in the duration of the respiratory cy

Clotting disorder - haemophilia, Clotting Disorder - Haemophilia Haemo...

Clotting Disorder - Haemophilia Haemophilia  is a congenital  blood clotting  disorder caused by the genetic lack/ deficiency of  coagulation factor VIII or antihaemophiliac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd