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Define Reagents - Nelson Somogyi Method for Glucose Estimation?
1. Alkaline copper reagent
Alkaline A- Buy commercial from SDS chemicals etc
Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.
Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use
Arsenomolybdate reagent
2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.
Unsaturated fatty acids Unsaturated fatty acids are liquid at room temperature and are characterized by having one or more double bonds. Fatty acids having two or more
It is a complex of antibody bound to antigen, which contains complement components.
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