Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Name the hormones produced by the testes, Name the hormones produced by ...

Name the hormones produced by (a) the testes, (b) the ovaries.   (a) The testes produce testosterone. (b) The ovaries produce oestrogen and progesterone.

Explain the management of furcal perforation, Explain the Management of Fur...

Explain the Management of Furcal Perforation 1 st isolate the perforation site. If the perforation is mechanical accidentally occurred:   a- It is probably

Explain malnutrition, Explain Malnutrition Malnutrition, impaired immun...

Explain Malnutrition Malnutrition, impaired immunity and infection can form a triad or vicious cycle, as illustrated in Figure, which works synergistically and thus worsens the

Define technique of paper chromatography, Define Technique of Paper chromat...

Define Technique of Paper chromatography? Paper chromatography is used as 1. Ascending, 2. Descending or 3. Horizontal technique (circular paper chromatography) It is De

Determine the final cell volume, Find the initial osmotic pressure at room ...

Find the initial osmotic pressure at room temperature for a cell if the only ions present are KCl and NaCl on either side of the membrane. Assume the concentrations for K+ and Na+

Effects of earthquakes on buildings, The dynamic response of the building t...

The dynamic response of the building to earthquake ground motion is the most important cause of damages to the building that are induced by earthquake. Failure of the ground and so

Explain normal nutrition - a base of therapeutic diet, Normal nutrition: a ...

Normal nutrition: a base of therapeutic diet Normal nutrition is the foundation upon which the  therapeutic modifications are based. The primary principle of diet nutrition the

Essay, Need an essay in regard to an experiment that I did. Essay Assignm...

Need an essay in regard to an experiment that I did. Essay Assignment: Experimental Design Now that you have completed several hands-on lab experiments, take a little time to con

When conducting the hair comparison, When conducting a hair comparison, wha...

When conducting a hair comparison, what factors would you consider?

Respiration, what is the organ of respiration in snaks

what is the organ of respiration in snaks

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd