Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Vesicular stomatitis, Vesicular stomatitis Vesicular sto matitis virus ...

Vesicular stomatitis Vesicular sto matitis virus be longs to the ge nus Vesiculovirus in fa mily Rhabdoviridae. The disease clinically resembles foot-and-mouth disease, but is

Nervous tissue, This constitutes about 2.4% of the body weight of man viz. ...

This constitutes about 2.4% of the body weight of man viz. Brain-1400 gram Spinal nerve-150 gram Spinal cord-30 gram Cranial nerve-10 gram C.S.F. is altogether not present in

The physical environment - stress of hospitalization, The Physical Environm...

The Physical Environment   The actual hospital environment is another factor that may be stressful for the child and family. There are many unfamiliar sights, sounds and smells

Growth and development, details and terms used in growth and development?

details and terms used in growth and development?

What is the purpose to use hydrocolloids, Hydrocolloids are extremely versa...

Hydrocolloids are extremely versatile and are used for many other purposes including: (a) Production of pseudoplasticity  (i.e. fluidity under shear) at high temperatures to ea

How have prokaryotic cells give origin to aerobic eukaryotic, How have prok...

How have prokaryotic cells given origin to aerobic eukaryotic cells and to photosynthetic aerobic eukaryotic cells? As per to the most accepted hypothesis aerobic eukaryotic ce

Loss-of-function mutations, Choose the correct answer. A. The phenotype det...

Choose the correct answer. A. The phenotype determines the genotype. B. True-breeding individuals are produced by repeated backcrossing. C. Recessive alleles are usually loss-of-fu

Malthusian theory of population growth, In 1798, British economist and demo...

In 1798, British economist and demographer Thomas Robert Malthus, published his' essay on population' in which he stated that: 1.      Population increases in a geometric rat

Nervous system - midbrain, MID BRAIN - Optics lobes 4 in number ( Corpo...

MID BRAIN - Optics lobes 4 in number ( Corpora Quadrizemmini ) in frog corpora bizemmini . Solid, optocoel is absent. 2 superior coculi and 2 inferior coculi are present.

Amount of intracellular chloride ions in neuron a, Amount of intracellular ...

Amount of intracellular chloride ions in neuron A Neuron A is a healthy neuron with all the usual ion channels.  When at rest with a membrane voltage of R millivolts, neuron A

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd