Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Define the nutritional assessment tools, Define the Nutritional Assessment ...

Define the Nutritional Assessment Tools? Malnutrition/protein energy malnutrition amongst elderly persons has been observed in various studies -be it hospitalized patients, nur

Explain regulatory enzymes, Explain Regulatory enzymes Regulatory enzy...

Explain Regulatory enzymes Regulatory enzymes:   Citrate  synthase,  isocitrate  dehydrogenase  and a-ketoglutarate dehydrogenase complex are the key  enzymes which  regulate

Endocrine interactions, Endocrine Interactions At the beginning of men...

Endocrine Interactions At the beginning of menstruation the inhibitory influence of the corpus luteum on the pituitary is removed and FSH is secreted in increasing amounts. Th

What is central shunt in palliative operations, What is Central Shunt in Pa...

What is Central Shunt in Palliative Operations? When the pulmonary artery branches are small, an interposition graft could be used as a shunt between main pulmonary artery and

Define disadvantages of using yeast as a source of protein, Define Disadvan...

Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma

Phylum Echinodermata, define and explain Water vascular system with podia a...

define and explain Water vascular system with podia and tube-feet madreporite mostly present

Is the epithelium vascularized, Is the epithelium vascularized? How do nutr...

Is the epithelium vascularized? How do nutrients and oxygen reach the epithelium? Why is this feature a significant evolutionary acquisition? Epithelia are not vascularized (ca

Excellent identification hypothesis for a plant tissue, What is the most ex...

What is the most excellent identification hypothesis for a plant tissue seen under the microscope having most cells undergoing cell division? The most excellent hypothesis is t

Explain the objectives of history of mart disease, Explain the OBJECTIVES o...

Explain the OBJECTIVES of history of mart disease? After reading this unit, you should be able to: 1. Understand the importance of Cardio-vascular diseases as the leading cause

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd