Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Define class cephalopoda in animal kingdom, Define Class Cephalopoda in ani...

Define Class Cephalopoda in animal kingdom? This group-including squids, octopuses, and the nautilus-is considered by most scientists to have the highest intelligence among all

What is severity and extent of lesion in cad, What is Severity and Extent o...

What is Severity and Extent of Lesion in coronaw artery disease? Apart from the high prevalence, the other disturbing features of CAD in South Asians are the severity and exten

Class Gastropoda, Class Gastropoda Body asymmetrical, depicts torsion ...

Class Gastropoda Body asymmetrical, depicts torsion or its effects; shell coiled in most, well developed head with radula, large flat foot, gills one or two or with pulmonary

Which enzyme acts on pyruvate in mitochondria, Which enzyme acts on pyruvat...

Which enzyme acts on pyruvate in mitochondria and converts it to acetyl CoA?Name its components and cofactors associated with it. Pyruvate dehydrogenase complex acts on pyruvate

Phases of ovarian cycle, Phases of Ovarian Cycle In non-mammalian vert...

Phases of Ovarian Cycle In non-mammalian vertebrates the ovarian cycle consists of the following phase; Pre breeding phase Breeding phase and Post breedin

Plant nutrition, what is usually regarded as evidence that photosynthesis h...

what is usually regarded as evidence that photosynthesis has occurred in a plant?

The kidney, diseases of the kidney and their remedies

diseases of the kidney and their remedies

Define the public nutrition, Define the Public Nutrition Public nutriti...

Define the Public Nutrition Public nutrition is concerned with improving nutrition in populations in both poor and industrialized countries, linking with continuity and public

Applications of various technologies in biotechnology, Question 1: Brie...

Question 1: Briefly explain the applications of various technologies in biotechnology. Definition of biotechnology Brief on different applications of various technolo

Dominance - synthetic characters, Dominance - Synthetic Characters It ...

Dominance - Synthetic Characters It is a characteristic of vegetation which expresses the predominating influences of one or more species in a stand so that the population of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd