Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Zoonoses disease-yabapox, Yabapox Yabapox is a disease affecting mangabeys...

Yabapox Yabapox is a disease affecting mangabeys, rhesus, cynos, vervets, stumptails, and patas monkeys. It is caused by the Yaba-like disease virus (YLDV) and Yaba monkey tumor v

Determine nutritional requirements in hot environments, Determine Nutrition...

Determine Nutritional Requirements in Hot Environments? Hot environments may be of two types: dry hot, as in the case of deserts or hot and humid in tropical rain forests and c

What''s the diffrence between regeneration and repair, What's the diffrenen...

What's the diffrenent between regeneration and repair ?  Regeneration: replacement of the destructed tissue by the same type of tissue  Repair : replacement of the destru

Human reproduction, Why is the human placenta referred to as haemochorial t...

Why is the human placenta referred to as haemochorial type? Name the hormone it secretes to facilitate parturition.

Define deuterostomes in animal kingdom, Define Deuterostomes in animal king...

Define Deuterostomes in animal kingdom? Coelomate animals are thought to have separated into two divergent lines of evolution in terms of the way embryos develop. In the embryo

In what ways does over-grazing lead to soil erosion, In what ways does over...

In what ways does over-grazing lead to soil erosion? Over-grazing leads to erosion because (i) the animals delete all the vegetation, so exposing the soil to heavy rain, (ii) t

Heart, Septa prevent oxygenated and deoxygenated blood. Give reason

Septa prevent oxygenated and deoxygenated blood. Give reason

Food intoxications, Food borne intoxications are basically food borne illne...

Food borne intoxications are basically food borne illness caused due to ingestion of toxin produced my microorganisms (mycotoxins, bacterial toxins). Natural toxins present in food

Define the meaning of goitrogens, Define the Meaning of Goitrogens? Goi...

Define the Meaning of Goitrogens? Goitrogens are substances that interfere with iodide metabolism in any way that inhibits thyroid hormone synthesis. As a result, there is augm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd