Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What si adenosine or dipyridamole thallium scan, Q. What si Adenosine or Di...

Q. What si Adenosine or Dipyridamole Thallium Scan? These studies are newer modalities that allow studying perfusion abnormalities to the myocardium when the patient cannot per

Slow moving waters - biota of rivers, Slow Moving Waters - Biota of Rivers ...

Slow Moving Waters - Biota of Rivers The habitat of a slowly moving part of the river is very different from the one just described. Here the water flow is comparatively slow

Preserving foods, One of man's oldest methods of preserving foods is drying...

One of man's oldest methods of preserving foods is drying or desiccation. The preservation of foods by drying is a direct result of removal of moisture. Microorganisms need water

Morphogenetic processes, Morphogenetic Processes We discussed earlier...

Morphogenetic Processes We discussed earlier the process through which a fertilized egg assumes the shape of a ball or a sheet of cells around a cavity, called blastula. Cons

Membrane perforation of sinus graft surgery, Membrane Perforation of sinus ...

Membrane Perforation of sinus graft surgery it is the most common complication during the sinus graft surgery resulting from the scoring of lateral access window for surgery an

Medication for cardiac patients, Medication The patient is advised to ...

Medication The patient is advised to continue antibiotics for a week more after discharge. Other medications prescribed are analgesics (acetaminopen), cough syrup, diuretics

Environment and health- impact of air and water pollution, Environment and ...

Environment and Health- Impact of Air and Water Pollution Environment includes a host of factors of which air and water are the two major components. Air pollution is caused b

Glycogen synthesis and degradation in liver, Glycogen synthesis and degrada...

Glycogen synthesis and degradation in the liver and muscle is very similar. Which of the following enzymes is not used in both muscle and liver for these metabolic pathways? -ph

Planning and implementation of nursing care-aplastic anaemia, Planning of N...

Planning of Nursing Care     Administer the drugs as prescribed -    Prevent infection    Control bleeding  Implementation of Nursing Care   Administer th

Inotropic agents, All currently available inotropic agents act to increase ...

All currently available inotropic agents act to increase Ca 2+ for activation in both normal and failing myocardium (Hurst). The use of inotropic agents in the treatment of CHF is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd