Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Protein prediction., I am trying to get help on the creation of a dataset f...

I am trying to get help on the creation of a dataset for protein prediction in matlab

Chemical properties of soil, CHEMICAL PROPERTIES OF SOIL Soil is a high...

CHEMICAL PROPERTIES OF SOIL Soil is a highly dynamic system which supports complex chemical reactions. In this heterogeneous systems, the soil solution acts as the medium for c

Characteristics of gametes, Characteristics of Gametes Following are s...

Characteristics of Gametes Following are some general characteristics of gametes: 1. The female gametes are usually laden with food material, the yolk, while the male gamet

Radioisotopes, Radioisotopes Radioisotopes are those forms of an elemen...

Radioisotopes Radioisotopes are those forms of an element which exhibit rzdioactivity and so can be used as markers or tracers. For this purpose, we first introduce the radiois

Explain lipid transport in nutritional care, Explain Lipid Transport in Nut...

Explain Lipid Transport in Nutritional Care? Proteins provide the transport mechanism for lipids by forming lipoproteins. This helps to prevent fatty infiltration and hence pro

Respiration, what is the organ of respiration in snaks

what is the organ of respiration in snaks

Effect of temperature, Effect of Temperature Certain plants such as th...

Effect of Temperature Certain plants such as the winter rye (Secale cereale) and the biennial strain of henbane (Hyoscyamus Niger) require exposure to low temperature conditio

Describe congenital pulmonary stenosis, Describe Congenital Pulmonary Steno...

Describe Congenital Pulmonary Stenosis? The murmur present since birth. PS may be valvular, sub-valvular, or supravalvular. An obstruction may occur within the RV cavity by an

Define thin layer chromatography, Define Thin Layer Chromatography? Thi...

Define Thin Layer Chromatography? This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr,

Increase in the divcat conductance of neuron b, Consider Neuron B in the fr...

Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectively conductive to a newly discovered divalent cation nam

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd