Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Explain about periimplant probing depth, Periimplant Probing Depth In c...

Periimplant Probing Depth In contrast to natural teeth, for which the average periodontal probing depth (PD) has been reported, the physiologic depth of the periimplant sulcus

Zoology, Phylum coelenterata

Phylum coelenterata

Define the enzyme function of vitamin c, Define the Enzyme Function of Vita...

Define the Enzyme Function of Vitamin C? Vitamin C acts as an electron donor for 11 enzymes. Three of those enzymes are found in fungi but not in humans. Of the eight remainin

What happens to the tsh blood level in hypothyroidism, What happens to the ...

What happens to the TSH (thyroid-stimulating hormone) blood level in hypothyroidism? Why is there enlargement of the thyroid in the endemic goiter disease? When there is low T3

Why pregnancy and lactation are critical stages in lifecycle, Why Pregnancy...

Why Pregnancy and lactation are critical stages in lifecycle? You must be aware that the situation of women is far from optimal and it is necessary to give attention to their n

Pre-requisites of counsellor, You have to do counselling of a diabetic pati...

You have to do counselling of a diabetic patient and the family members when you are working in a diabetic clinic or community setting in health center. To be an effective counsell

Explain the meaning of anthropometric measures, Explain the Meaning of anth...

Explain the Meaning of anthropometric measures Anthropometric Measures mean and its uses. The term Anthropometric refers comparative measurement relating to the body structure.

What is the name of process of deposition, Thickness and toughness of plant...

Thickness and toughness of plant walls are increased by deposition within original cellulose wall, of a substance called as lignin. Process of deposition is called: a) Lignific

What is the main biological process, Q. What is the main biological process...

Q. What is the main biological process that consumes carbon dioxide? The major biological process that consumes carbon dioxide is photosynthesis.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd