Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Name the t major groups of cells, Name the t major groups of cells required...

Name the t major groups of cells required in attaining specific immunity. Name the end products of aerobic and anaerobic glycolysis List two ways by which molecules of ATP are

Lithosphere, The lithosphere contains all of the hard solid land of the ear...

The lithosphere contains all of the hard solid land of the earth crust and the semisolid land underneath the crust. The surface of the lithosphere is very uneven contains high moun

Bile acid sequestrants for coronary prevention, These are safe and free of ...

These are safe and free of systemic side effects. However, gastrointestinal side effects are common, and compliance is poor. The average LDL decreases by approximately 15 per cent

Enumerate about the colour vision defects, Enumerate about the colour visio...

Enumerate about the colour vision defects Acquired colour vision defects can occur afkr damage to the optic nerve or macula and in some cases, because of blue blindness in old

Transplant procedure and immunosuppression, TRANSPLAN T PROCEDURE - Op...

TRANSPLAN T PROCEDURE - Operation is done under general anaesthesia, duration 3-4 hrs. Cut is given in the lower abdomen. Donar's kidney is transplanted retroposistonicaly

How can coacervates be formed of phospholipids, How can coacervates be form...

How can coacervates be formed of phospholipids or polypeptides? Phospholipids are amphipathic molecules, i.e., they present a polar portion and a nonpolar portion. In contact w

#titleprotozoans, Ask question #Minimum 100 words accwhat is protozoans ep...

Ask question #Minimum 100 words accwhat is protozoans epted#

Describe the uses-process and outcome of pgd, 1.  Identify and compare the ...

1.  Identify and compare the methods of somatic gene therapies for ADA deficiency described on pp. 404-405 (Lewis) and OTC deficiency described on pp. 405-406 (Lewis). 2.  Compa

What is the prothallus of pteridophytes, Q. What is the prothallus of pteri...

Q. What is the prothallus of pteridophytes? The gametophyte develops by mitosis from a spore and Prothallus is the pteridophyte gametophyte (the haploid individual that forms g

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd