Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Define briefly about the nuts as protein source, Define briefly about the N...

Define briefly about the Nuts as protein source? Nuts are excellent sources of proteins. Examples of nuts include cashew nuts, almond nuts , hazel nuts , coconuts, walnuts, bra

Pericarditis - nursing management, Pericarditis Nursing Management ...

Pericarditis Nursing Management Monitor-blood pressure, Pulse temperature, respiration, chills disphoresis, jugular pressure. Assess for signs of cardiac tarnponad

Budding- asexual reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Pituitary gland, PITUITARY GLAND (HYPOPHYSIS CEREBRI) - It develops ...

PITUITARY GLAND (HYPOPHYSIS CEREBRI) - It develops from ectoderm of the embryo. The pituitary gland is located just below the hypothalamus. The pituitary gland is situate

Could this be accomplished by passive diffusion, In the human body, the pot...

In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t

Explain Genetic equilibrium for genes with two alleles, What is the mathema...

What is the mathematical expression of the genetic equilibrium for genes with two alleles? Is this statistical distribution the same as the statistical distribution of the respecti

Illustrate numerical taxonomy, Q. Illustrate Numerical Taxonomy? Taxono...

Q. Illustrate Numerical Taxonomy? Taxonomy today is, in many dctails, different from what it was a generation ago. The use of computers by taxonomists has established an intere

Define obligatory and facultative water excretion, Define Obligatory and Fa...

Define Obligatory and Facultative water excretion? i) Obligatory water excretion: The kidney is 'obligated' to excrete some water to rid the body of its daily load of urinary s

Total artificial heart in circulatory assist devices, Total Artificial Hear...

Total Artificial Heart :  Jarvik seven was the first successful total artificial heart supporting the patient for 112 days. The disadvantage is that the patient has to be connecte

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd