Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What is stenotic and regurgitant lesions, Q. What is Stenotic and regurgita...

Q. What is Stenotic and regurgitant lesions? The normal cardiac valves offer little resistance to blood flow even when flow velocity is high. When stenosis develops, the valve

What is the energy source used in active transport, What is the energy sour...

What is the energy source used in active transport through biological membranes? The energy essential for active transport (against the concentration gradient of the transporte

Where does calvin cycle take place in chloroplast, Where does Calvin cycle ...

Where does Calvin cycle take place in chloroplast? Explain the cycle. a) Where is electron transport system operative in mitochondria? b) Explain the system highlighting the

Phanerophytes - classes of life form, Phanerophytes - Classes of Life Form ...

Phanerophytes - Classes of Life Form The perennating buds in this case are present on erect, negatively geotropic shoots, much above the ground. These buds are naked or least

Explain nutrition diagnosis, Explain Nutrition diagnosis Nutrition dia...

Explain Nutrition diagnosis Nutrition diagnosis is the identification and labelling that describes an actual occurrence, risk of, or potential for developing a nutrition probl

What are the types of colloidal dispersions, What are the types of colloida...

What are the types of colloidal dispersions Depending upon the relative affinity of the dispersed phase for the dispersion medium, colloidal dispersions are, further divided in

What is the difference between amnion and chorion, What is the difference b...

What is the difference between amnion and chorion? Amnion is the membrane that covers the embryo. Chorion is the membrane that covers the amnion, the yolk sac and the allantois

What were genotypes of the f2 plants crosses, A snapdragon plant that bred ...

A snapdragon plant that bred true for white petals was crossed to a plant that bred for purple petals, and all the F1 had white petals. The F1 was selfed. Among the F2, three other

Explain the pre pregnancy height and foetal outcome, Explain the Pre Pregna...

Explain the Pre Pregnancy Height and Foetal Outcome? Maternal height prior to conception, determined by heredity, socio-economic environment and maternal nutritional status, is

Class of mollusca - aplacophora, Class of Mollusca - Aplacophora Worm...

Class of Mollusca - Aplacophora Worm-like, no shell, head or excretory organs mantle with chitinous cuticle or scales or spicules mantle cavity posterior. Aplacophorans are a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd