Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What is the leaf cuticle, What is the leaf cuticle? The leaf cuticle is...

What is the leaf cuticle? The leaf cuticle is a thin waxy layer made of cutin and waxes on the outer surface of the leaf epidermis. Its function is to control the cellular tran

Evolution, what is r and k selection

what is r and k selection

CRUSTACEA, Respiratory system of Crustacea ?

Respiratory system of Crustacea ?

Describe the uses-process and outcome of pgd, 1.  Identify and compare the ...

1.  Identify and compare the methods of somatic gene therapies for ADA deficiency described on pp. 404-405 (Lewis) and OTC deficiency described on pp. 405-406 (Lewis). 2.  Compa

How old is the earth, How old is the earth? It is believed that the ear...

How old is the earth? It is believed that the earth is about 4.5 billion years old.

Arterial conduits and internal mamnrary artery (ima), Arterial Conduits ...

Arterial Conduits LIMA UMA: anastomosis side-t-side with diaganal8 end-lo-sode with LAD Figure: Arterial conduits Internal Mamnrary Artery (IMA)

Theory of germplasm, THEOR Y OF GERMPLASM - August Weismann (1834-1...

THEOR Y OF GERMPLASM - August Weismann (1834-1914) criticized the inheritance of acquired characters by putting forward the theory of continuity of germplasm. According

Define role of vitamin k in controlling gene expression, Define role of Vit...

Define role of Vitamin K in controlling gene expression? Prithrombin, a protein required for blood clotting is assembled with a large number of glutamic acid residues. In the p

Explain procedure for simple staining of bacterial cultures, Explain Proced...

Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s

What are the main causes of mutation, Q. What are the main causes of Mutati...

Q. What are the main causes of Mutation? Some mutations take place randomly and spontaneously during process of mitosis or meiosis. Others are caused by mutagenic agents that a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd