Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Determine the function of iron for athletes, Determine the function of Iron...

Determine the function of Iron for athletes? Iron carries oxygen and is another important mineral for athletes. It also affects oxygen transport and aerobic metabolism as a com

fat digestion requires two steps, Fat digestion requires two steps? What a...

Fat digestion requires two steps? What are the steps and what enzymes are used to accomplish each step?

How much fluids should be taken for management of obesity, How much Fluids ...

How much Fluids should be taken for management of obesity? Liberal amounts of water and zero/low calorie fluids may be included in the diet. It may be helpful to have a glass o

Why are additives used in foods?, Normal 0 false false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Infective endocarditis, Despite improvements in health care, the incidence ...

Despite improvements in health care, the incidence of infective endocarditis has not decreased over the past decades. Infective endocarditis is lethal if not aggressively treated w

What is genomics , We are living in an unprecedented age of biological ...

We are living in an unprecedented age of biological discovery and the application of biological knowledge.   Programmed DNA sequencing delivered, in the year of  2001 over the 2.6

Air pollution- introduction, Air pollution is complex in origin and varied ...

Air pollution is complex in origin and varied in effect. It is very crucial from the point of human health. Every human being inhales about 15-22 kg of air daily and air is pollute

How does sexual reproduction occur in bacteria, How does sexual reproductio...

How does sexual reproduction occur in bacteria? How different are the modalities of bacterial sexual reproduction? Sexual reproduction happens when bacteria incorporate genetic

Physiology of excretion, PHYSIOLOGY OF EXCRETION - 1 .      FORMATION...

PHYSIOLOGY OF EXCRETION - 1 .      FORMATION OF UREA - It takes place in liver. (i) Deamination (ii) Ornithine cycle or urea cycle - Explained by H.

Pulse oximetry - diagnostic tests, Pulse Oximetry: Procedure: Pulse Ox...

Pulse Oximetry: Procedure: Pulse Oximetry is a safe and  simple method of assessing oxygenation. An  advantage is  that this method  is noninvasive and continuous. Previously,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd