Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What vector foce does particle b then exert on a, A charged particle A exer...

A charged particle A exerts a force of 2.39uN to the right on charged particle B when the particles are 12.3 mm apart. Particle B moves straight away from A to make the distance be

Structure of myofibril, STRUCTUR E OF A MYOFIBRIL - The dark bands ...

STRUCTUR E OF A MYOFIBRIL - The dark bands of the myofibril are termed the A-bands (Anisotropic bands). Each A-band has at its middle a light zone called H-zone (Henson'

Viriods and prions, Viriods and Prions Viroids        In 1971, T.O...

Viriods and Prions Viroids        In 1971, T.O.Diener reported that the  spinde tuber disease of potato causing gnarling and cracking of the tuber, is caused by multiplica

State the active hyperemia, Which of the following is true for active hyper...

Which of the following is true for active hyperemia, a local control mechanism within the circulatory system? A. There will be a decrease in force developed by smooth muscles s

What are the positions of actin and myosin molecules, What are the position...

What are the positions of actin and myosin molecules in the sarcomere before and during the muscle contraction? Schematically actin filaments attached perpendicularly to both s

Describe integrative systems of the body, Q. Why is the endocrine system co...

Q. Why is the endocrine system considered one of the integrative systems of the body? What is the other physiological system that also has this function? The endocrine system i

Respiration - metabolism of pollen tubes, Respiration - Metabolism of Polle...

Respiration - Metabolism of Pollen Tubes In the unpollinated pistils of Hippeastrum hybridum very high O 2 , tension exists from stigma down through most of the style. During

Name the molecules make active transport through membranes, Which are the m...

Which are the molecules that make possible active transport through membranes? Active transport is made by exact membrane proteins. These proteins are called "pumps" because

Which glands secrete their product into circulatory system, Name of the duc...

Name of the ductless glands that secrete their product into the circulatory system are: a) Exocrine (pron: ek-seh-kren) b) Apocrine (pron: ap-eh-kren) c) Holocrine (pron:

Principle for determination of blood glucose, Define Principle for Determin...

Define Principle for Determination of Blood Glucose by Nelson Somogyi Method? Proteins are precipitated from the sample and protein free filtrate is heated with alkaline copper

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd