Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Classification of chromatography -basic separation technique, Define Classi...

Define Classification of Chromatography - Basic Separation Technique Chromatography can be classified depending upon: Physiochemical principles/factors, The techniqu

Digestive system - salivary glands, SALI V A R Y GLAND - These are ...

SALI V A R Y GLAND - These are compound - Alveolar, tubular glands made up of columnar / cuboidal epithelium. Saliva is manufactured in them. In mammals these are pai

Animal and plant cells, Animal and Plant Cells The internal structural ...

Animal and Plant Cells The internal structural organisation and information related with hereditary characters and metabolic pathways are similar in all eucaryotic cells whethe

What is mitral valve and coronary arteries, Q. What is Mitral Valve and Cor...

Q. What is Mitral Valve and Coronary Arteries? On the PA view, mitral valve calcification is seen just to the left of the spine, below the position of the aortic valve. The lar

Leaf, Which one of the following is an example of a tree having simple leaf...

Which one of the following is an example of a tree having simple leaf ? 1. Neem 2. Rose 3. Mimosa pudica 4, Peepal.

Difference between ectoparasite and endoparasite, Q. What is the difference...

Q. What is the difference between ectoparasite and endoparasite? The Ectoparasites are parasites that explore the external surface of the host (like, for instance, mites that p

Plant physiology, what is the importance of lignin in xylem formation

what is the importance of lignin in xylem formation

Define the process of digestion of proteins, Define the Process of Digestio...

Define the Process of Digestion of Proteins? The daily protein intake (of about 50-100 g) and the protein of enzymes, sloughed (bled or drop off) epithelial cells and mucins, w

What are the five kingdoms, Q. What are the five kingdoms into which living...

Q. What are the five kingdoms into which living beings are divided? And which group of living being is out of this classification? The five kingdoms of living beings are the ki

Advantage and disadvantage of steam sterilization, Q. Advantage and Disadva...

Q. Advantage and Disadvantage of steam sterilization? Advantage: most efficient and reliable sterilization method available. Its quite simple to operate and relatively inexpens

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd