Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What do you mean by synapses, Q. What do you mean by synapses? Synapses...

Q. What do you mean by synapses? Synapses are the structures that transmit the neural impulse between two neurons. When the electric impulse arrives the presynaptic membrane

Phylum:Echinodermata , What classifies an animal into the Echinodermata phy...

What classifies an animal into the Echinodermata phylum?

Mechanism of blood coagulation, GENERA L MECHANISM OF BLOOD COAGULATION - ...

GENERA L MECHANISM OF BLOOD COAGULATION - All researchers agree that blood clotting is a cascade mechanism and involves three essential steps - (a) Formation of prothrombin ac

Regular soda are made with aluminium, If the cans of regular soda are made ...

If the cans of regular soda are made with aluminium than diet soda, then regular soda will sink. Write brief descriptions of how you could test this hypothesis.

Explain about the radiation therapy, Explain about the Radiation Therapy? ...

Explain about the Radiation Therapy? Radiation therapy is one of the options for the treatment of various tumors. During the administration of radiation patients experience acu

Define dietary management of cancer patients, Define Dietary Management of ...

Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.

Determine the toe corticospinal interneuron, Which of the following is true...

Which of the following is true for a toe corticospinal interneuron that produces action potentials during movements of the big toe of the left foot? A. A portion of its axon is

Determine fragment of nucleic acid has a nucleotide sequence, If a fragment...

If a fragment of nucleic acid has a nucleotide sequence TAC can one assert that it is a codon or an anticodon? A nucleic acid having a TAC sequence surely is not tRNA, it is DN

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd