Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Post-mating isolating mechanisms, The second category of isolating mechanis...

The second category of isolating mechanism that may permit interspecific mating but ensures reduced viability is called post-mating isolating mechanism. This category could be subd

Define the recommended dietary allowance for thiamin (rda), Define the Reco...

Define the Recommended Dietary Allowance for Thiamin (RDA)? Thiamin, as it must be clear by now, is needed mainly for the metabolism of carbohydrate, branch-chained amino aci

Hypothalamus, Hypothalamus functions are generally aimed at maintaining A ...

Hypothalamus functions are generally aimed at maintaining A region in the brain beneath the thalamus; consists of number of aggregations of nerve cells and controls a variety of a

Which are the respiratory muscles in mammals, Q. Which are the respiratory ...

Q. Which are the respiratory muscles in mammals? In mammals the muscles that participate in the breathing process are the diaphragm and the intercostal muscles, in respiratory

How are arthropods characterized, Q Concerning germ layers and the presence...

Q Concerning germ layers and the presence of coelom how are arthropods characterized? Arthropods are triploblastic and coelomate beings (they have three germ layers). Q. Co

Instances of human diseases caused by bacteria, Q What are instances of hum...

Q What are instances of human diseases caused by bacteria? Some human diseases caused by bacteria are tuberculosis, pertussis, diphtheria, gonorrhea, bacterial meningitis, bubo

Define about the chemotherapy, Define about the Chemotherapy - Cancer? ...

Define about the Chemotherapy - Cancer? Chemotherapy results in lot of side effects. This is because the drug effects are not specific to cancer cells alone. Even the host cell

Which elements contains carbohydrates, (a) Which elements contains Carbohyd...

(a) Which elements contains Carbohydrates (b) Which elements contains Proteins   a) Carbohydrates contain the elements carbon , hydrogen and oxygen. b) Protei

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd