Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Growth phase -stages in spermatogenesis, Growth Phase -Stages in spermatoge...

Growth Phase -Stages in spermatogenesis Growth phase is characterized by the acquisition of the structural and functional characteristics of distinct sex cells. Also here is a

Type of bacterial food poisoning, Type of bacterial food poisoning In...

Type of bacterial food poisoning Infection type: The infection type results from the release of toxin in the bowel by microorganisms taken in foods, e.g., Salmonella or Vibr

Flatworms, What are the adaptions flatworms neeed in order to live in their...

What are the adaptions flatworms neeed in order to live in their environment?

Explain the reinforcement of patch using fibrin glue, Explain the Reinforce...

Explain the Reinforcement of Patch using Fibrin Glue? Reinforcement of Patch using Fibrin Glue :  In this technique Dacron graft is first sutured to the septum beyond i

Intravenous drug abusers, The risk for IE among IV drug abusers, 2 to 5 per...

The risk for IE among IV drug abusers, 2 to 5 per cent per patient- year, is estimated to be several fold greater than that of patients with rheumatic heart disease or prosthetic v

............., notochotr is absent in the group ...........

notochotr is absent in the group ...........

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd