Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Energy metabolism, Energy Metabolism In the preceding sections of the ...

Energy Metabolism In the preceding sections of the unit, you studied how the products of digestion of food, viz: amino acids, sugars and fatty acids are absorbed and transport

Embryological evidences of evolution, EMBRYOLOGICAL EVIDENCES - A co...

EMBRYOLOGICAL EVIDENCES - A comparative study of embryology of different groups of animals reveals certain features that provide evidences for organic evolution. There ar

AUTOCHORY, ALL ABOUT AUTOCHORY?HOW IT WORKS

ALL ABOUT AUTOCHORY?HOW IT WORKS

Starch, S T ARC H - Polymer of a-D-glucose. Starch is glucosan...

S T ARC H - Polymer of a-D-glucose. Starch is glucosan homopolysaccharide which is the major reserve food of plants. Starch is formed as an end product of photosynt

The basics of dna cloning, There  are a broad  range  of various  process  ...

There  are a broad  range  of various  process  for cloning  DNA  into  either viral  or plasmid vectors  but the basic  scheme  of events  is frequently  same.  To clone into a pl

Determine the term - angiomas, Determine the term - Angiomas Angiomas a...

Determine the term - Angiomas Angiomas are congenital collections of abnormal vessels that divert the normal flow of blood. These capillaries, venous, or arteriovenous (A-V) ma

Describe pressure controlled ventilation, Describe Pressure Controlled Vent...

Describe Pressure Controlled Ventilation (PCV) This is a pressure cycled mode (machine delivers a set pressure - the tidal volume generated depends on how compliant the lungs

SYMBIOTIC THEORY, WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A ...

WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A VERY SIMPLE WAY.

Explain the net flux of bicarbonate, Which of the following processes in ca...

Which of the following processes in capillaries in the lung assist in the removal of carbon dioxide from the body? A. Net flux of bicarbonate from plasma into red blood cells.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd