Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

What are social dangers, Members belonging to the scientific community fear...

Members belonging to the scientific community fear the misuse of this therapy leading to dangerous consequences.  People may try to insert the desired gene, for example, the gene f

Before purchasing - nursing software, Before Purchasing  Ideally it...

Before Purchasing  Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions.   A totally Custom-made softwar

What are the hormones produced by the testicles, What are the hormones prod...

What are the hormones produced by the testicles and the ovaries? The testicles make androgenic hormones, the major of them being testosterone. The ovaries make estrogen and pro

Explain about the shade drying, Explain about the Shade Drying? Shade d...

Explain about the Shade Drying? Shade drying is carried out for products which can lose their colour and/or turn brown if put in direct sunlight. Products that have naturally v

What is the mechanism of elisa, ELIZA stands for enzyme linked immune diffu...

ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.

What is the acrosome of the sperm cell, What is the acrosome of the sperm c...

What is the acrosome of the sperm cell? How is it formed? The acrosome is a structure that has a great number of digestive enzymes, it is located in the anterior end of the spe

Clinical features of gout disease, Q. Clinical Features of gout disease? ...

Q. Clinical Features of gout disease? You may be enjoying good heath but may suddenly get a severe attack. You wake up in the middle of the night, and your big toe feels as if

What is the difference among dna and rna, Concerning the nitrogen-containin...

Concerning the nitrogen-containing bases that participate in nucleotides, what is the difference among DNA and RNA? In DNA nucleotides can be formed of adenine (A), thymine (T)

What is the main risk factor for skin cancer, Q. What is the main risk fact...

Q. What is the main risk factor for skin cancer? The major risk factor for skin cancer is solar exposition of the skin without protection against ultraviolet radiation (a poten

Excretory system, what is the excretory system of a crab?

what is the excretory system of a crab?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd