Define reagents-nelson somogyi method for glucose estimation, Biology

Assignment Help:

Define Reagents - Nelson Somogyi Method for Glucose Estimation?

1. Alkaline copper reagent

Alkaline A- Buy commercial from SDS chemicals etc

Alkaline B- Weigh 6.25 g anhydrous Na2CO3 and 6.25 g Na - K tartarate, 5 g sodium arsenate and 50 g Na sulphate anhydrous. Dissolve them in distilled water one by one and make vol to 250 ml.

Alkaline C- Mix reagents A (4 vol) and B (1 vol) just before use

Arsenomolybdate reagent

2. Dissolve 25 g ammonium molybdate in 450 ml water. Carefully add 21 ml concentrated H2SO4 with stirring. Dissolve 3 g Na2HAsO4.7H2O in 25 ml water and add to ammonium molybdate solution. Incubate at 37° C and store.

 


Related Discussions:- Define reagents-nelson somogyi method for glucose estimation

Morphology, Morphology:  It deals with external forms, structure and the re...

Morphology:  It deals with external forms, structure and the relative position of various organs of living beings. Morphology is a branch of bioscience which dealing with the study

Define need of vitamin c during pregnancy period, Define need of vitamin C ...

Define need of vitamin C during pregnancy period? Vitamin C deficiency has not been shown to affect the course or outcome of pregnancy in humans, although low plasma levels hav

Define isomerases, Isomerases These  enzymes (code  EC 5)  catalyze geo...

Isomerases These  enzymes (code  EC 5)  catalyze geometric  or  structural  changes  within  a molecule. According  to the  type of  isomerism,  they  may be  called  racemases

Selective destruction - wildlife, Selective Destruction - Wildlife The...

Selective Destruction - Wildlife The selective destruction of one species of an existing fauna can produce equally unfortunate results. The perfect demonstration of unexpected

What do you mean by ventricular arrhythmias, Q. What do you mean by Ventric...

Q. What do you mean by Ventricular Arrhythmias? Considerable disagreement exists regarding the significance of resting ventricular ectopic beats. In study comparing the coronar

Cellular evaluation of blood test, Cellular evaluation of blood test  F...

Cellular evaluation of blood test  Full blood count (or "complete blood count")  Hematocrit or packed cell volume (PCV) - This is the fraction of whole blood volume that con

Threatened species - wildlife, Threatened Species - Wildlife Many plan...

Threatened Species - Wildlife Many plant and animal species are threatened by the possibility of extinction. However, the seriousness of the threat varies. For example, a spec

Define tuberculosis, Define tuberculosis Tuberculosis (TB) is still a p...

Define tuberculosis Tuberculosis (TB) is still a problem in the United States, even though the incidence continues to decline in most of the country. Treatment of TB can be div

How to use a key, Q. How to Use a Key? The use of a key is analogous to...

Q. How to Use a Key? The use of a key is analogous to travelling a high way, that forks repeatedly, each fork having roadside directions. If a traveller follows the proper dire

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd