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Define Reagents required and Methodology for Fehling's Test?
- Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose, starch
- Fehling A (Copper sulphate solution)
- Fehling B (A solution containing KOH and sodium potassium tartarate)
Methodology
Mix 2 ml of Fehling A with 2 ml of Fehling B just before use in a test tube. Add about 10 drops of sugar solution and boil for about a minute.
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Although fructose does not have an aldehyde group it reacts first of all bonds of fructose was breaked by fehling solution then fructose coverted to aldehydic group then that
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