Define procedure for detection of number of bacteria in milk, Biology

Assignment Help:

Define Procedure for Detection of Number of Bacteria in Milk?

1. Place a clean, non-greasy slide over the graph paper.

2. Spread 0.01 ml of the milk over an area of 1 cm2 as a thin even film.

3. Dry the smear by gently passing through the flame.

4. Defat the smear by flooding with xylol for 1 minute.

5. Wash the slide with alcohol to remove xylene followed by washing with water to remove the alcohol.

6. Stain the smear with methylene blue for 15-30 second.

7. After washing with water, observe the dried preparation under oil immersion. Count the number of bacteria in about 25 microscopic field.

8. Determine the average number of bacteria per microscopic field and multiply with microscopic factor (500,000) to get bacterial counts per ml of the milk sample.


Related Discussions:- Define procedure for detection of number of bacteria in milk

Male reproductive system - semen, SEMEN - Sperms and secretion of acces...

SEMEN - Sperms and secretion of accesory glands collectively known as seminal fluid or semen. It is milky, semi-solid in nature having particular smell. pH : 7.35 - 7.5. Spe

Soil temperature, Soil Temperature Soil temperature is known to influe...

Soil Temperature Soil temperature is known to influence water absorption and ultimately transpiration to a considerable extent. In many plants water absorption is reduced shar

Post-operative care of cardiac surgical patients, POST-OPERATIVE CARE OF PA...

POST-OPERATIVE CARE OF PATIENTS The first few days following cardiac operations are the most critical in terms of the patient's survival. The safety with which a patient can

Genetics, how does the universality of the genetic code make recombinant DN...

how does the universality of the genetic code make recombinant DNA technology possible

Advantage and disadvantages of fensom and spanner, Advantage and disadvanta...

Advantage and disadvantages of Fensom and Spanner Electroosmotic Flow Hypothesis The model has several advantages over Munch's model. The presence of P- proteins, and the occl

What are the biotechnological applications of enzymes, Biotechnological app...

Biotechnological applications of enzymes Although enzymes have been used in certain industrial processes for centuries, their precise role or even their identity was not known

Removal of impurities in water, Many common methods are employed for the re...

Many common methods are employed for the removal of impurities from water. Some of them are: Sedimentation Filtration Reverse osmosis Electro dialysis Aeration

Explain hepatitis a, Hepatitis A Hepatitis A vaccine (Havrix - GlaxoSm...

Hepatitis A Hepatitis A vaccine (Havrix - GlaxoSmithKline; Vaqta Merck) is recommended for all susceptible travelers going anywhere other than Canada, Australia, New Zealand,

Procaryotic structure and diversity, Procaryotic Structure and Diversity ...

Procaryotic Structure and Diversity The first organisms which did not have well defined nucleus, called procaryotes, (pro = earlier, caryon = nucleus) were probably anaerobic a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd