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Define Ion exchange chromatography?
In this method a reversible exchange of ions is effected. Usually resins and ionic solutes are the two components involved in ion-exchange. Ion exchange resins are of two types cation and anion exchange resins. The acidic or cation exchange resins contain sulfonic acid groups (SO3H), carboxylic groups (COOH) or phenol groups (OH).
Basic or anion exchange resins contain weakly basic amine groups (NH2) or strongly basic quarternary ammonium groups (NH4)+ OH-. The surfaces of acid resins bind cations such as H, Na+, Ca2+, NH4+. The basic or anion exchange resins carry positive charges on the surface and thus adsorb negative ions.
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Endocrine Glands
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In designing an experiment to find out whether light is required for photosynthesis (a) what is the principle of the design (b) what control would you use?
When glucagon binds its receptor there is an increase in the second messenger, cAMP. cAMP levels can be decrease by which of the following enzymes? -adenylate cyclase -PDK1
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