Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

In how many categories nutrients are divided, In how many categories nutrie...

In how many categories nutrients are divided? The nutrients are grouped into three categories: 1) Energy Yielding: Carbohydrates and fats produce energy in the body. 2) B

Find which two amino acids make up this pocket, Sickling occurs in deoxyhem...

Sickling occurs in deoxyhemoglobin, but not in oxyhemoglobin. Normal deoxyhemoglobin (HbA and HbS) molecules have a small hydrophobic "pocket" on the surface of a ? chain. In oxyhe

Determine the high blood plasma levels of insulin, A patient has total dest...

A patient has total destruction of all of the beta-islet cells of the pancreas.  Prior to receiving treatment, this patient will have A. high blood plasma levels of glucose.

Some common air pollutants: particulates, Particulates: These are suspe...

Particulates: These are suspended droplets, solid particles or mixture of two. So all the atmospheric substances that are not gases are called particulates. A number of term

Why is the cleavage in mammals as simple homoplastic, Why do the sons of a ...

Why do the sons of a haemophiliac father never vary from this trait? Why is the cleavage in mammals referred as simple homoplastic?

Explain precautions for estimation of reducing sugar, Explain Precautions f...

Explain Precautions for Estimation of Reducing Sugar by Fehling Soxhlet Method? 1. Clamp the burette so that the tip of the burette is exactly above the mouth of the conical fl

Bovine parainfluenza, Bovine parainfluenza This virus belongs to the g...

Bovine parainfluenza This virus belongs to the genus Respirovirus in the subfamily Paramyxovirinae of family Paramyxoviridae. It causes respiratory syndrome in cattle and shee

Ways in which genetic recombination occurs during meiosis, Define two ways ...

Define two ways in which genetic recombination occurs during meiosis. Genetic recombination happens during crossing- over and independent assortment.

Define low water permeability across the luminal membranes, A healthy perso...

A healthy person is on a diet that having of very small amounts of water. A. This person will make a large volume of dilute urine with low amounts of dissolved solutes. B. T

DNA, what is the genetic code of life

what is the genetic code of life

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd