Define citrate utilization test - imvic test, Biology

Assignment Help:

Define Citrate Utilization Test - imvic test?

Several microorganisms have the ability to make use of citrate as the sole source of carbon and energy. This ability relies on the presence of citrate permease and citrase in the organism. Citrate permease makes easy the transport of citrate in the cell while citrase acts on citrate to produce oxaloacetic acid and acetate as pointed out in the reaction.

Citrate utilization through microorganisms can be detected by using Simmons citrate medium that consists of sodium citrate as the sole carbon source and bromothymol blue like pH indicator. When citrate in the medium is utilized by microorganisms, resulting CO2 reacts with sodium and water to form sodium carbonate. This alterations the pH of the medium to alkaline and its colour from green to deep prussian blue. Bromothymol blue is green while acidic (pH 6.8 and below) and blue while alkaline (pH 7.6 and higher). 


Related Discussions:- Define citrate utilization test - imvic test

Define function of thiamin as regulator of enzyme activity, Define Function...

Define Function of thiamin as Regulator of enzyme activity? Thiamin regulates the enzymes involved in carbohydrate metabolism. These are: a) Pyruvate dehydrogenase, which pr

Controlling expression of a number of genes in e, he Lac Repressor protein,...

he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of

Stomata - water loss, Stomata - Water Loss The cross-section of a leaf...

Stomata - Water Loss The cross-section of a leaf shown in Figure shows the position of a typical stoma (plural stomata) which however, differs from species to species, with re

Reproduction, what are different forms of asexual reproduction?

what are different forms of asexual reproduction?

Breifly describe the menstrual cycle, Breifly describe the Menstrual Cycle?...

Breifly describe the Menstrual Cycle? Each month during a female's reproductive years, the hypothalamus secretes a hormone that stimulates the anterior lobe of the pituitary gl

Fundamental difference between protozoans and algae, What is the fundamenta...

What is the fundamental difference between protozoans and algae? The basic difference among protozoans and algae is the fact that protozoans are heterotrophs whereas algae are p

Why probability and likelihood in a hidden markov model, Please explain to ...

Please explain to me why the probability and the likelihood in a hidden Markov model are so different.

Bitter taste, Q. Bitter Taste? Bitterness is an inherent property of a ...

Q. Bitter Taste? Bitterness is an inherent property of a substance. It is due to substances such as alkaloids present in food. For example, tannins present in tea, coffee, frui

Ecology, (i) On Figure, draw a ring around the number which indicates the e...

(i) On Figure, draw a ring around the number which indicates the energy entering the system via photosynthesis. (ii) The total energy available to the plants in the ecosystem

What is the basic need for bone grafting, What is the basic need for bone g...

What is the basic need for bone grafting The basic need for bone grafting includes situations like: 1. Implant site development- when the quantity of bone is deficient to s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd