Define about the paper electrophoresis, Biology

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Define about the Paper electrophoresis?

In paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate moistened with buffer solution. The ends of the strip are immersed in separate reservoirs of buffer in which the electrodes are placed. Upon application of a direct current the ions of the sample migrate towards the electrodes of opposite polarity to form discrete bands. An ion's migration rate is influenced to some extent by its interaction with the support matrix, but is largely a function of its charge upon completion of the electrophoretogram, the strip is dried and stained to detect the sample components. In high voltage paper electrophoresis the paper is clamped between two cooled plates to carry off the heat generated by the high-voltage current flow.


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