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• Cancer cells do not respond normally to the body's control mechanism.
o They divide excessively and invade other tissues.o If left unchecked, they can kill the organism.
• Cancer cells do not exhibit contact inhibition
o If cultured, they continue to grow on top of each other when the total area of the petri dish has been covered o They may produce required external growth factor (or override factors) themselves or possess abnormal signal transduction sequences which falsely convey growth signals thereby bypassing normal growth checks.
• Cancer cells exhibit irregular growth sequences
o If growth of cancer cells does cease, it does so at random points of the cell cycle o Cancer cells can go on dividing indefinitely if they are given a continual supply of nutrients o Normal mammalian cells growing in culture only divide 20-50 times before they stop dividing.
What volume of 4x protein sample buffer do you need to add to 15 ml of a cell extract in order to load it on a gel?
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