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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
Q. Rigid fixation to evaluate osseointegration? Is a clinical term that means absence of observed mobility. Rigid fixation indicates the absence of clinical mobility of an impl
Release of lysosomal enzymes from white blood cells during a local immune attack can contribute to the symptoms of inflammation. Suppose, to alleviate inflammation, you develop a d
For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a jo
Q. Acute diarrhoea? It must be evident from the table above that acute diarrhoea generally occurs in association with infections, poisons and drugs. Chronic diarrhoea on the ot
Epidemiology: The study of disease in populations. Includes: The Aetiology of the disease Patterns of distribution of the disease How the disease spread
55yrs old woman had burns and has lost 6% body fluid.She weighs 50Kg.How much fluid does she need to replace the lost fluid.
Congenital disorders Poor fertilizing quality of semen could be due to impaired spermatogenesis. When such a condition is due to genetic congenital causes the treatment is not
Draw a diagram of the longitudinal section of a mature anatropous ovule and label any ten parts in it.
Aerobic and Anaerobic Exercises Generally, there are two types of exercises: - Aerobic - Anaerobic Aerobic exercises are those in which the patient spends calories fro
What are the secondary roots? In origin how the secondary roots differentiate from shoots? The Secondary roots are ramifications of the primary (major) root. The secondary root
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