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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
what are golgi apparatus
Explain Procedure for Spore Staining in a Given Bacterial Culture Carry out the exercise following the steps enumerated herewith 1. Take a clean, non-greasy slide. Prepare b
Q. How many chambers does the fish heart have? The fish heart is a tube made of two consecutive chambers such as one ventricle and one atrium.
A woman is heterozygous for three X-linked loci: AaBbDd. She has seven sons with the following genotypes: two are X ABd Y three are X abD Y one is X ABD Y one is X Abd
Absence of vesicular glands In some bulls the vesicular glands are either absent or hypoplastic also sometimes accompanied by poorly developed ampullae. A characteristic sympt
Q. How are bacteria are classified according to their need for oxygen? According to their need of oxygen bacteria are classified into anaerobic those that survive without oxyge
what is the name of the valve that separates the chamber?Why is that valve necessary?
EVOLUTION OF MAN - Mammals evolved from primitive reptiles (therapsida) in Triassic period, about 210 million years ago. Mammals existed as inconspicuous group of small
Q. Discuss the advantages of implant supported maxillo-facial prosthesis. Maxillofacial Prosthodontics involves the prosthetic rehabilitation of patients with congenital or acq
What is the name of the larva of corals and sea anemones? What is the biological significance of that larval stage? Sexual reproduction in corals and sea anemones has a larval
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