Cat assay, Biology

Assignment Help:

CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.


Related Discussions:- Cat assay

Energy demand for sedentary or light activity lifestyles, Explain Energy de...

Explain Energy demand for Sedentary or Light Activity Lifestyles? These people have occupations that do not demand much physical effort, are not required to walk long distances

Specify the term in detail - swim bladder, Specify the term in detail - Swi...

Specify the term in detail - Swim bladder. Found in bony fish, this gas-filled chamber is used to main neutral buoyancy. Oxygen in blood is added or removed as required. Swim b

Vasa gene, Vasa are a maternal-effect gene whose product functions in devel...

Vasa are a maternal-effect gene whose product functions in development of the posterior end of the Drosophila embryo.  runt is a non-maternal-effect gene whose product functions in

What is the enzyme reverse transcriptase present in hiv, Q. Which type of v...

Q. Which type of virus is the HIV? What is the enzyme reverse transcriptase present in HIV? HIV is a retrovirus that is RNA viral (its genetic material is RNA and not DNA).

Homopolysaccharides, HOMOPO L YSACCHARIDE S (= HOMOGLYCANS) These...

HOMOPO L YSACCHARIDE S (= HOMOGLYCANS) These are complex carbohydrats that are formed by repeated condensation or polymerisation of only one type of monosaccharide monome

Explain briefly nucleic acids dna and rna, Explain briefly Nucleic Acids (D...

Explain briefly Nucleic Acids (DNA & RNA)? Nucleic Acids (DNA & RNA) :  Nucleic acids are the molecules that contain genetic information that is inherited, and are responsibl

Locomotion in protozoa, give a detail account of modes of locomotion in pro...

give a detail account of modes of locomotion in protozoa

Qualitative test for specific sugars, what is another test for pentoses? wh...

what is another test for pentoses? what is the positive result for this test?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd