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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
Phycomycosis (zygomycosis) Zygomycosis (previously known phycomycosis) involves fungi belonging to genera Mucor, Rhizopus, Absidia, Entomophthora, Mortierella and Basidiobolou
elementatr cannal and feeding mechenism
Explain Infants and Preschool Children Growth? In this unit we will be studying about one of the crucial phases of human growth and development i.e. infancy to preschool years.
Define the term - magnetoencephalography A variant ERP known as magnetoencephalography (MEG) has been developed. MEG, which is still in its infancy, requires upward of 60 elect
mitochondria?
Introduction: There are different technologies available for dealing with waste water management and excreta. Some are suitable for dealing off-site sanitation and other can b
Explain the term Brown-Sequard Syndrome Brown-Sequard syndrome is a rare spinal disorder that results from an injury to one side of the spinal cord in which the spinal cord is
Explain Strengthening the public health measures? Parasitic infestations and protozoal infections cause iron deficiency through loss of blood or destruction of red blood cells.
What are some human diseases caused by bacteria and what are their respective modes of transmission? The major human bacterial infections transmitted by respiratory secretions
Soy Protein Concentrates The Association of American Feed Control Officials, Inc. (AAFCO) specifies soy protein concentrates as follows: "Soy Protein Concentrate is organiz
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