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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
Determine Water-soluble vitamins needs of school children and adolescents? The suggested requirements are given in Table 15.2 (ICMR 1990). Thiamine is computed as 0.5 mg/1000 K
how trematodes/nematodes adapt to their parasitic mode of feeding
sanguivarous mode of feeding in annelida
Q. What do you mean by Polyester? Polyester (ES) or polyethylene terephthalate (PET): PET is a very strong transparent glossy film, which has good moisture and gas barrier p
what is the classificatin of phylum protozoa with orders
Compare and contracte the phylum of ceolantrate
Agreed-upon Procedures - Non-assurance Engagements Within an engagement to present agreed-upon procedures, the auditor is engaged to carry out those procedures of an audit nat
Types of Biogeochemical Cycles There are two basic types of biogeochemical cycles, gaseous and sedimentary. In the gaseous type of biogeochemical cycle there is a prominent ga
Q. What is the special route that lipids follow during digestion? What are chylomicrons? Triglycerides emulsified by the bile within micelles suffer the action of lipases that
Q. How do arthropods grow? Due to the presence of exoskeleton the growth of an arthropod is periodical during the growth period the animal loses the exoskeleton, develops and g
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