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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
What is phototropism? Phototropism is the movement of plant structures in response to light. Phototropism might be positive or negative. Positive phototropism is that in which
A protein is normally found completely within the inside of a lipid bilayer. The protein likely has: a) Mostly hydrophobic side chains, pointed outwards, with the peptide backbo
How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal
In rats, the gene pigment (P) is dominant to no pigment (p). The gene for black (B) is dominant to the gene for cream (b). If a pigment gene (P) is absent, genes B and b are inoper
Bone Modeling It is a surface-specific activity (apposition or resorption) that produces a net change in the size and/or shape of a bone after initial bone formation in the emb
Extra-ocular muscles There are, as you know, six extra-ocular muscles. Out of these, four are recti(straight) and two are oblique. They control the position and movement of th
Define the Magnesium Requirements in body? Let us learn about the magnesium requirements. Since plant foods are particularly high in magnesium, on a vegetarian diet with plenty
short notes on cholesterol.
Solubility of Gases in water Most gases which are important for biological processes dissolve readily and specially in water. The solubility of any gas in water generally var
What is the typical localization of the tropical forests regarding latitude? Tropical rain forests, as the Amazon forest and the Congo forest, are typically located in low lati
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