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Q. Can you explain Lane and Eynon method?
Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.
Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.
These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.
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Chronic Renal Failure (CFW) Chronic renal failure begins when the diseased kidney can no longer maintain the normal chemical structure of body fluids under normal conditio
Q. Effect on Microbial Growth of pH? Every microorganism has a minimal, a maximal, and an optimal pH for growth. In general, bacteria grow in the pH range of 6.0-8.0, yeasts 4
Q. What is wall resistance of plant cells? Does this resistance make difficult the entrance of water into the cell or facilitate? Turgor Pressure TP, or Wall resistance, is the
Explain saturated fatty acids Some of the more significant saturated fatty acids with their systematic and common names are listed in table, and some of the unsaturated fatty a
Empiric Initial Therapy Until susceptibility results are available, empiric initial treatment consists of a 4-drug regimen of isoniazid, rifampin, pyrazinamide and ethambutol.
State about polyploid cells During development cells of certain tissues like the salivary gland and liver produce extraordinary amounts of selected proteins. This is often achi
1. Noise producing industries, railway stations, and aerodromes should be located away from the residential areas. 2. Check on the traffic in the residential colonies.
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Research and Documentation - Conservation of wildlife First, list of endangered species are established by various national and international agencies. Another important actio
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