Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Determine environmental factors of viscosity and plasticity, Environmental ...

Environmental factors of viscosity and plasticity a) Temperature affects the viscosity of a colloid. Generally, its viscosity decreases as the temperature increases. For e.g.

Humans which distinguish them from other primates, State two characteristic...

State two characteristics of humans which distinguish them from other primates. Two features which differentiate humans from other primates are their upright posture and their

Scrapie, Scrapie This is a chronic fatal disease of sheep and occasiona...

Scrapie This is a chronic fatal disease of sheep and occasionally of goats caused by a Prion agent similar to BSE. The disease is reported from Europe, North America and in a f

What is the relationship between enzyme, Q. What is the relationship betwee...

Q. What is the relationship between enzyme and vitamins cofactors? Many vitamins are enzyme cofactors that cannot be synthesized by the organism and must be acquired from the d

Explain transposition the great arteries with ventricular, Explain Transpos...

Explain Transposition the Great Arteries with Ventricular Scptal Defect ? Transposition of the great arteries with VSD is an indication for surgery. It is preferable to do VSD

Name the rich source of aerobic bacteria, 1. Biogas is produced by the acti...

1. Biogas is produced by the activity of aerobic bacteria on animal waste 2. Methanobacterium is an aerobic bacterium found in rumen of cattle 3. Biogas, commonly called goba

What is the life cycle type of bryophytes, What is the life cycle type of b...

What is the life cycle type of bryophytes? As in all plants the life cycle of bryophytes is diplobiontic (alternation of generations). In bryophytes the lasting form is the hap

Describe the advantages of clinical neuropsychology, Describe the advantage...

Describe the advantages of Clinical neuropsychology Clinical neuropsychology seeks like as understanding, particularly, in the case of how damaged or diseased brain structures

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd