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A pesticide inhibits the activity of a particular enzyme, which can therefore be used to assay for the presence of the pesticide in an unknown sample.
a) In the laboratory, the initial rate data shown in the table below were obtained. What type of inhibitor (competitive, uncompetitive, non-competitive) is the pesticide? Determine the values of Vmax, KM, and KI. v, M/min x 106 S, M No inhibitor 10-5 M inhibitor 3.3 x 10-4 56 37 5.0 x 10-4 71 47 6.7 x 10-4 88 61 1.65 x 10-3 129 103 2.21 x 10-3 149 125
b) After 50 ml of the same enzyme solution in part (a) is mixed with 50 ml of 8x10-4 M substrate and 25 ml of a test sample, the initial rate observed is 18 µM/min. What is the pesticide concentration in the test sample assuming no other inhibitors or substrates are present?
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A 100-turn coil in the configuration of Figure P12.1.3 is rotated at a constant speed of 1200 r/min in a magnetic field. The rms induced voltage across the coil is 1 kV, and each turn has a length l = 112.5 cm and a width w = 10 cm. Determine the ..
Calculate the parameters of the equivalent circuit shown in Figure 13.2.5(c).
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At Chernobyl in 1986, hydrogen was produced by the reaction of superheated steam with the graphite reactor core: C(s)+H2O(g) yields CO(g)+H2(g) It was not possible to prevent a chemical explosion at Chernobyl
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Describe two potential options for improving the blood compatibility of biomaterials, discuss their potential for ultimately improving blood compatibility and describe any engineering challenges to implementing these to practice.
question 1the effect of a hazardous substance depends on the concentration of the substance that is present in the
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Calculate the ratio of the network over the amount of heat absorbed at the high temperature of the cycle. How does this value compare to the theoretical efficiency of the Carnot cycle that is calculated based on the two operating temperatures?
Discuss the possible individuals and/or organizations that may have been responsible for this injury.
A third-order Butterworth low-pass filter is shown in the following circuit with an ideal operational amplifier. Find the magnitude of the output
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