Use recombinant dna methods to prepare a mutant

Assignment Help Biology
Reference no: EM13526740

A drug company decided to use recombinant DNA methods to prepare a mutant

a1-antiproteinase (also sometimes referred to by its old name, a1-antitrypsin) that would be moreresistant to oxidation than the naturally occurring inhibitor, to administer intravenously to emphysemapatients. What single amino acid substitution would you recommend, and why?

Reference no: EM13526740

Questions Cloud

Amino acid sequence of this polypeptide : The amino acid sequence of this polypeptide was determined in series of mutants listed in parts a. through e. For each mutant, indicate the type of change that occurred in the DNA (single-base substitution, insertion, deletion) and the phenotypic ..
Introduction of electrons to the electron transport chain : With the introduction of electrons to the Electron Transport Chain, the first molecules in the chain present in NADH dehygrogenase complex, would be __________ when the electron as passed to the next molecule?
What is most likely genotypic arrangement of genes in mother : Red-green colorblindness is the most common form of colorblindness and is caused by two closely linked genes located near the tip of the human X chromosome. Red blindness, or protanopia, and green blindness, or deutanopia, are recessive conditions..
Determine the genotypes of the parents : Black coat color in Cocker Spaniels is governed by a dominant allele and red coat by a recessive allele; solid pattern is governed by the dominant allele of another gene and spotted pattern is recessive.
Use recombinant dna methods to prepare a mutant : A drug company decided to use recombinant DNA methods to prepare a mutant a1-antiproteinase (also sometimes referred to by its old name, a1-antitrypsin) that would be moreresistant to oxidation than the naturally occurring inhibitor, to administer..
For the definitions-synarthrotic-amphiarthrotic : for the definitions: synarthrotic, amphiarthrotic, and diarthrotic joints detailing the type of movement and location of each....
The enzyme papain is a thiol protease : The enzyme papain is a thiol protease, using a mechanism similar to the mechanism used by chymotrypsin, except that there is an active site Cys in papain carrying out the catalytic role played by the active site Ser of chymotrypsin.
Calculate the fraction of the total enzyme active sites : Calculate the fraction of the total enzyme active sites in solution that would be in the active form at pH 7.2. Calculate what kcat would be if the activity of the enzyme were measured at pH 7.2.
responsible for the pH effect on kcat : Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step

Reviews

Write a Review

Biology Questions & Answers

  Select the reduced-fat product as part of a healthy diet

In this case, we are tracking an enzyme secreted by pancreatic cells. What is its most possible pathway. Find out how many calories you will have saved by eating this reduced-fat product instead of the high-fat one.

  Solving genetics problem

In rats, gene B produces black coat color if the genotype is B-, but black pigment is not manufactured if the genotype is bb.

  Find the genotype frequency in the second generation

In a population of flowers growing in a meadow, C1and C2 are autosomal codominant alleles that control flower color. The alleles are polymorphic in the population.

  Where are the male and female gametophytes located

Where are the male and female gametophytes located on a Peyote(Lophophora Williamsi)?

  Technique to separates rna and dna

Discuss and explain why is it difficult to separate large amounts of RNA and DNA using gel electrophoresis?

  How does dna encode information

how does DNA replication ensure genetic constancy during cell division.

  Make a table comparing the corresponding chemoautothroph

Note that chemoautotroph bacteria has a an opposite counterpart in heterotroph bacteria that use anaerobic respiration ( have an electron aceptor an inorganic compound different from oxygen).

  List the steps of the complement fixation test

List the steps of the complement fixation test. include diagrams for each step, contrasting the results for a positive sample versus a negative?please help me.

  Name the two general categories of stems cells and

q1. how do stem cells differentiate from other cells?q2. name the 2 general categories of stems cells and identify

  What is the parental generation for the mapping experiment

What is the parental generation for the mapping experiment? What assumptions are you making for the parental generation of the mapping experiment? Give the phenotype and genotype of parental generation.

  Define the directed mutation hypothesis

Explain the directed mutation hypothesis (1). Describe an experiment that has tested this hypothesis (2). What was the outcome of this experiment (1)? What did this experiment teach us about how mutation and natural selection interact during evolu..

  Explain two ways that mutations can affect the phenotypes

Explain two ways that mutations can affect the phenotypes that does not include alteration of the protein coding sequence.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd