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The enzyme papain is a thiol protease, using a mechanism similar to the mechanism used by chymotrypsin, except that there is an active site Cys in papain carrying out the catalytic role played by the active site Ser of chymotrypsin. There is a His residue in papain that plays the same role as the active site His residue in chymotrypsin.1) Name the region of the chymotrypsin active site responsible for tight transition state binding in chymotrypsin’s chemical mechanism.2) Like chymotrypsin, papain should also have a region responsible for binding/stabilizing the transition state in its peptide hydrolysis mechanism. Use your knowledge of the mechanism of chymotrypsin to name the structural element of the transition state that would be tightly bound by papain. (You don’t need to draw a structure.)
A reactio mixture containing 20 mM O-acetly serine, fifty mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine is incubated at 37 C until the O-acetyl serine is completely hydrolyzed.
The structure of biological membranes is estimated primarily by the lipid bilayer but the specific functions of these membranes are carried out through proteins associated with the membrane.
It is estimated that the human genome contains 25,000 genes and the average length of the coding sequence of a gene is 3,000 base pairs. Using these numbers, determine what percentage of the genome is in genes.
The following cross involving three genes was performed: Aa Bb Cc x aa bb cc, and the phenotypes and number of progeny are shown below. Each pair of alleles exhibits simple dominance and recessiveness.
Coat color in cats is a codominant trait and is also located on the X chromosome. Cats can be black, yellow or calico. A calico cat has black and yellow splotches. In order to be calico, the cat must have an allele for the black color and an allel..
What type(s) of reproductivebarriers are most likely at play in reproductive isolation of the two species- Prezygotic or postzygotic
Describe the process of viral replication; discuss both normal and retroviruses, and comment on the implications of this process for genetic engineering.
The normal eye color of Drosophila is red, but strains in which all flies have brown eyes are available. Similarly, wings are normally long, but there are strains with short wings.
q1. thirteen-year-old ryan has begun to challenge many of his parents values and to express his own set of highly
q1. dna fingerprinting identifies individual bears and also allows for determining gender of the bear. what information
Supposethey added the enzyme to a solution of 90%CTP and 10%UTP, and then used a cell-free system of protein synthesis to make peptidesaccording to the sequences in their synthetic RNAs
What do chloroplasts and mitochondria have in common
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