Reference no: EM133932348

Questions

1. In the lab, we usually transfect our gene of interest linked to an antibiotic resistance gene to select out any untransfected cells with antibiotic-treated cell media. However, we can't quite do this with multicellular organisms like mice. Aside from genotyping your mouse, think of a strategy that would allow you to tell whether your transfected mouse truly is transgenic (it expresses the DNA you transfected it with). Hint: consider visual cues.

2. PI is demonstrating lentiviral transfection methods and accidently jabs her finger with a syringe containing lentivirus encoding influenza proteins. She tells you not to worry about it, and the next few days when you see her at lab she seems perfectly fine. How come your PI didn't get the flu or experience flu-like symptoms? Propose two reasons and explain any assumptions you've made.

3. You are differentiating 3T3-L1 cells into adipose-like cells. To induce them, you need to change the cells' media with DMEM (commonly used basal medium for many different mammalian cells)+FBS, in addition to adding dexamethasone, IBMX, and insulin. Your lab has the stock solutions: 10000X dexamethasone, 100X IBMX, and 1000X insulin. How much dexamethasone, IBMX, and insulin (in microliters) do you need to add to 75 ml of DMEM/FBS? You want each of the stock solutions to be at a 1x concentration. Hint: use C1V1=C2V2.