Bind long non-palindromic asymmetric dna sequences

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Question

Options are: MN / megaTAL,ZFN,TALEN,CRISPR/Cas9

1. What endonucleases can bind long non-palindromic asymmetric DNA sequences and create a DSB?

2. Which endonuclease has been successfully used in yeast to perform DSBR via HDR?

3. What disadvantage do these enzymes have over CRISPR/Cas9 in the context of precision genome editing?

Reference no: EM133932347

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