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1. In southern Manitoba, the population would likely have a higher tolerance to low temperatures in October, as they have adapted to the colder climate and overwintering. The curve for this population in October would likely have a minimum tolerable temperature that is lower than the other two populations.
2. In South Dakota, the population would likely have a lower tolerance to low temperatures in October, as they are not adapted to the colder climate and do not overwinter. The curve for this population in October would likely have a minimum tolerable temperature that is higher than the other two populations.
3. The curve for the southern Manitoba population collected in the middle of August would likely lie between the other two curves, as the temperature tolerance for this population would be intermediate between the two populations.
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