### High-pressure liquid chromatography

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##### Reference no: EM131034322

part A )

High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 4.24×108 Pa , what is its running pressure in torr?
Express the pressure numerically in torr.

Part B)

If the volume of the original sample in Part A (P1 = 622 torr , V1 = 34.0 L ) changes to 67.0 L , without a change in the temperature or moles of gas molecules, what is the new pressure, P2?
Express your answer with the appropriate units.

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