General and confocal microscopy and image analysis

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Reference no: EM133999116

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General and Confocal Microscopy and Image Analysis

1. Provide a detailed description of one of the methods in general microscopy (hint: phase, dark-field, bright-field). Describe (i) the name of the method (ii) the experimental arrangement of the method (with a diagram), (iii) the physical basis of the method (what kind of light matter interaction) (iv) what produces contrast in the method ( one pg limit)

2. Consider the following objective lens types (100X, NA 1.2, 60X NA 1 or 40X NA 0.75). Which lens has the best magnification? Which lens has the best resolution? What is the approximate resolution of the 100X NA 1.2 lens (consider 500nm light) ( one pg limit)

Reference no: EM133999116

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