Explain the rabbit brain tissue was homogenized

Reference no: EM13498402

Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column. This is an affinity column in which ATP is covalently linked to a polysaccharide bead. This sample is loaded on top of the column, washed with a low-salt buffer, followed by a wash with a high salt buffer. What is the rationale for this procedure and what would the expected elution profile look like? (Keep in mind PFK-1A Mr = 84000 dal, PFK-1B Mr = 80000dal, PFK-1C Mr=86000 dal)

Reference no: EM13498402

Questions Cloud

Explain what rate of disappearance of no2 would be predicted : The decomposition of NO2 by the following reaction at some temperature proceeds at a rate 5.40 x 10-5 mol NO2/L.s when [NO2] = 0.01144 mol/L.

What will his angular speed be when they are tucked in : An ice skater has a moment of inertia of 2.5 kg*m2 when his arms are outstretched and a moment of inertia of 1.0 kg*m2, what will his angular speed be when they are tucked in

Determine the best possible way to account for inflation : Determine the best possible way to account for inflation when considering where to invest your money. Provide specific examples to support your response

Find the charge on the plates before and after immersion : A parallel-plate capacitor in air has a plate separation of 1.50 cm and area of 25.0 cm^2. find the charge on the plates before and after immersion

Explain the rabbit brain tissue was homogenized : Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column.

Compute the position of the image : An object 0.680cm tall is placed 18.5cm to the left of the vertex of a convex spherical mirror having a radius of curvature of 25.0cm. Calculate the position of the image

Evaluate the average speed of car : Imagine that you took a road trip. Based on the information in the table, what was the average speed of your car

Calculate the assessable - deductible amount : Necktie Pty Ltd (Necktie), an Australian resident company, had the following transactions in the year ended 30 June 2012.

Define taxation law : Calculate Caroline's taxable income for the year ended 30 June 2012. Show workings where relevant and briefly explain all inclusions and exclusions.

User Account

All Pages