Describe how genetic diversity in bacteria develops

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Reference no: EM133999040

Questions

1. Chapter 9

Define all bold-faced terms in the text

Describe:

• the structure of DNA
• the process of DNA replication
• the purpose of DNA replication
• the central dogma of DNA and how information flows in the cell

Describe the processes of transcription and translation and their overall purpose in the cell.

Describe how genes expression is regulated and discuss phenotypic variation (lac and trp operons)

Describe how genetic diversity in bacteria develops.

Describe the different types of mutation and know how they classified based on changes in DNA, and how they are classified based on changes to the protein.

Discuss the causes of mutation, and the effects of mutation.

Describe the 3 types of gene transfer. Discuss the impact gene transfer has on 1) bacterial cells and 2) human health. Discuss why gene transfer and mutation are necessary for evolution of bacteria.

2. Chapter 10

Define all bold-faced terms in the text.

Describe how PCR works and be able to compare/contrast PCR to DNA replication in the cell.

Discuss applications of PCR.

Describe how restriction enzymes work and how they can generate RFLPs.

Describe how DNA fingerprints are generated and how they are visualized.

Describe how gel electrophoresis works.

Describe how molecular probes work.

3. Chapter 11

Define all of the bold faced terms in the text.

Know the 5 targets of antimicrobial drugs and examples of each.

Know the 6 characteristics of an ideal drug. Understand that no drug is "ideal".

Describe the mechanism of action of beta-lactam drugs.

Describe Synergism.

Be able to discuss why M. tuberculosis is difficult to treat.

Discuss the various genetic causes of drug resistance (from Ch. 9).

Describe the mechanisms of drug resistance (p. 421-422)

Discuss methods for slowing the emergence of drug resistance.

Describe the 3 targets of antiviral drugs (as per your text) and know examples of each.

Describe the primary target of antifungal drugs, and discuss why there are so few of these drugs.

Discuss why there are so few antiprotozoal and antihelminthic drugs.

Describe how to carry out MIC and MBC tests.

Describe the E test.

Describe how to achieve and interpret a Kirby-Bauer test.

Reference no: EM133999040

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