Demonstrate one gene-one enzyme hypothesis

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Question

1. How did Beadle and Tatum's experiments on Neurospora crassa demonstrate the one gene-one enzyme hypothesis?

2. Indicate the major classes of RNA transcripts and the role of each of these RNAs.

3. Where do transcription and translation occur in prokaryotes and eukaryotes? What are prokaryotes able to do because they don't have a nucleus (with respect to transcription and translation)?

4. Draw a double stranded DNA molecule with the 5' and 3' ends labeled (line drawing) and then indicate which will be your template strand and your coding (non-template strand) by drawing an RNA molecule that will be made by the DNA. Also label the ends of your RNA molecule with 5' and 3'.

5. What happens at each stage of transcription?

6. How is the pre-mRNA modified in RNA processing?

7. Indicate how the spliceosome can be considered a ribozyme.

8. What is the functional and evolutionary significance of having introns?

9. What does the wobble position in the codon and what is going on here?

10. What are two ways accuracy is maintained in translation?

11. Indicate the major features of the genetic code.

Start codon
Stop codons

What does it mean that it is degenerate?

12. What are the 3 sites of the ribosome involved in translation?

13. Indicate the major events of each stage of translation

Reference no: EM133935283

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